Simultaneous detection of different Rhizobium strains marked with either the Escherichia coli gusA gene or the Pyrococcus furiosus celB gene.

Enterotoxigenic Escherichia coli (ETEC) strains which produce heat labile and/or heat stable toxins (LT and ST) may cause diarrhea in humans and farm animals. Using PCR primers specific for the LT I and ST II genes, a multiplex PCR system which allows detection of LT I- and ST II-producing ETEC strains was developed. When skim milk was used for a PCR assay, it was found that if target cells in the sample were precultured in MacConkey broth for 8 h prior to PCR as few as 100 cells per ml of the sample could be detected. Without the preculture step, 104 CFU of target cells per 0.2 g of porcine stool specimen were required to generate visible PCR products. The multiplex PCR System can be used for rapid testing of fecal specimens, food and possibly environmental samples for the presence of ETEC strains.

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Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli

Gene ◽

10.1016/0378-1119(95)00128-s ◽

1995 ◽

Vol 160 (1) ◽

pp. 101-103 ◽

Cited By ~ 2

Author(s):

Carol E. Jones ◽

Toni M. Fleming ◽

Peter W. Piper ◽

Jennifer A. Littlechild ◽

Don A. Cowan

Keyword(s):

Escherichia Coli ◽

Pyrococcus Furiosus ◽

High Homology ◽

Cloning And Sequencing ◽

Gene Encoding

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Analyzing the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus

JBIC Journal of Biological Inorganic Chemistry ◽

10.1007/s00775-009-0471-2 ◽

2009 ◽

Vol 14 (4) ◽

pp. 573-585 ◽

Cited By ~ 6

Author(s):

Sanghamitra Mitra ◽

George Sheppard ◽

Jieyi Wang ◽

Brian Bennett ◽

Richard C. Holz

Keyword(s):

Escherichia Coli ◽

Pyrococcus Furiosus ◽

Specific Inhibitors

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New Medium for the Simultaneous Detection of Total Coliforms and Escherichia coli in Water

Applied and Environmental Microbiology ◽

10.1128/aem.59.12.4378-4378c.1993 ◽

1993 ◽

Vol 59 (12) ◽

pp. 4378-4378

Keyword(s):

Escherichia Coli ◽

Simultaneous Detection ◽

Total Coliforms

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Use of a multiplex PCR system for the simultaneous detection of heat labile toxin I and heat stable toxin II Escherichia coli cells in environmental waters near Taichung City

Journal of Food and Drug Analysis ◽

10.38212/2224-6614.2907 ◽

2020 ◽

Vol 6 (2) ◽

Author(s):

S.-W. Wang ◽

C.-C. Tsai ◽

H.-Y. Tsen

Keyword(s):

Escherichia Coli ◽

Multiplex Pcr ◽

Simultaneous Detection ◽

Environmental Waters ◽

Heat Stable ◽

Escherichia Coli Cells ◽

Heat Labile ◽

Taichung City

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Rapid, Specific, Defined Substrate Technology Colilert System for the Simultaneous Detection of Total Coliforms and Escherichia coli from Water

Rapid Methods and Automation in Microbiology and Immunology ◽

10.1007/978-3-642-76603-9_53 ◽

1991 ◽

pp. 436-443

Author(s):

S. C. Edberg ◽

M. J. Allen ◽

D. B. Smith

Keyword(s):

Escherichia Coli ◽

Simultaneous Detection ◽

Total Coliforms

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Analysis of Bottled Water for Escherichia coli and Total Coliforms

Journal of Food Protection ◽

10.4315/0362-028x-61.3.334 ◽

1998 ◽

Vol 61 (3) ◽

pp. 334-338 ◽

Cited By ~ 9

Author(s):

M. A. GRANT

Keyword(s):

Escherichia Coli ◽

Simultaneous Detection ◽

Microbiological Quality ◽

Bottled Water ◽

Plate Count ◽

Heterotrophic Plate Count ◽

Positive Error ◽

False Positive Error ◽

Total Coliforms ◽

Quality Guidelines

U.S. Food and Drug Administration regulations governing bottled water include microbiological quality guidelines based on coliform counts. Recently, a new MF medium for simultaneous detection of total coliforms and Escherichia coli was developed. This medium, m-ColiBlue24 (m-CB) was compared to m-Endo medium and an International Organization for Standardization standard coliform medium, lactose agar with Tergitol 7. Coliform analysis was conducted on 104 brands of bottled water from 10 countries. Some samples were additionally analyzed for heterotrophic plate count and Pseudomonas sp. populations, including P. aeruginosa. Presumptive coliform colonies were found in 5.8% of the samples with m-CB, 1.9% with m-Endo and 11.5% with lactose agar with Tergitol 7. None of the presumptive coliforms from any of the three media were verified as true coliforms in subsequent analysis. Consequently, the presumptive recovery rates actually represented false-positive error (FPE) rates. The FPE for m-CB and m-Endo were not statistically different (P < 0.05) but the FPE for lactose agar with Tergitol 7 was significantly larger.

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A Novel DNA Polymerase Family Found inArchaea

Journal of Bacteriology ◽

10.1128/jb.180.8.2232-2236.1998 ◽

1998 ◽

Vol 180 (8) ◽

pp. 2232-2236 ◽

Cited By ~ 69

Author(s):

Yoshizumi Ishino ◽

Kayoko Komori ◽

Isaac K. O. Cann ◽

Yosuke Koga

Keyword(s):

Escherichia Coli ◽

Dna Polymerase ◽

Pyrococcus Furiosus ◽

Dna Polymerases ◽

Open Reading Frames ◽

Gene Products ◽

Polymerase Gene ◽

Hyperthermophilic Archaeon ◽

Dna Polymerase Ii ◽

Reading Frames

ABSTRACT One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeonPyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499–512, 1997). The genes in M. jannaschiiencoding the proteins that are homologous to the DNA polymerase II ofP. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia colihad both DNA polymerizing and 3′→5′ exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

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