Enzymatic Manganese(II) Oxidation by a Marine α-Proteobacterium

ABSTRACT A yellow-pigmented marine bacterium, designated strain SD-21, was isolated from surface sediments of San Diego Bay, San Diego, Calif., based on its ability to oxidize soluble Mn(II) to insoluble Mn(III, IV) oxides. 16S rRNA analysis revealed that this organism was most closely related to members of the genus Erythrobacter, aerobic anoxygenic phototrophic bacteria within the α-4 subgroup of theProteobacteria (α-4 Proteobacteria). SD-21, however, has a number of distinguishing phenotypic features relative to Erythrobacter species, including the ability to oxidize Mn(II). During the logarithmic phase of growth, this organism produces Mn(II)-oxidizing factors of ≈250 and 150 kDa that are heat labile and inhibited by both azide ando-phenanthroline, suggesting the involvement of a metalloenzyme. Although the expression of the Mn(II) oxidase was not dependent on the presence of Mn(II), higher overall growth yields were reached in cultures incubated with Mn(II) in the culture medium. In addition, the rate of Mn(II) oxidation appeared to be slower in cultures grown in the light. This is the first report of Mn(II) oxidation within the α-4 Proteobacteria as well as the first Mn(II)-oxidizing proteins identified in a marine gram-negative bacterium.

Download Full-text

Occurrence and distribution of polycyclic aromatic hydrocarbons in surface sediments of San Diego Bay marinas

Marine Pollution Bulletin ◽

10.1016/j.marpolbul.2016.10.009 ◽

2017 ◽

Vol 114 (1) ◽

pp. 466-479 ◽

Cited By ~ 14

Author(s):

Carlos Neira ◽

Jennifer Cossaboon ◽

Guillermo Mendoza ◽

Eunha Hoh ◽

Lisa A. Levin

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Aromatic Hydrocarbons ◽

San Diego ◽

Surface Sediments ◽

San Diego Bay ◽

Polycyclic Aromatic

Download Full-text

TEM study of a biofilm on copper corrosion

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100163563 ◽

1996 ◽

Vol 54 ◽

pp. 220-221

Author(s):

W. A. Chiou ◽

N. Kohyama ◽

B. Little ◽

P. Wagner ◽

M. Meshii

Keyword(s):

Marine Bacterium ◽

Culture Medium ◽

Copper Alloys ◽

Pure Copper ◽

Localized Corrosion ◽

Natural Seawater ◽

Copper Corrosion ◽

New Approach ◽

The Past ◽

Copper Tolerant

The corrosion of copper and copper alloys in a marine environment is of great concern because of their widespread use in heat exchangers and steam condensers in which natural seawater is the coolant. It has become increasingly evident that microorganisms play an important role in the corrosion of a number of metals and alloys under a variety of environments. For the past 15 years the use of SEM has proven to be useful in studying biofilms and spatial relationships between bacteria and localized corrosion of metals. Little information, however, has been obtained using TEM capitalizing on its higher spacial resolution and the transmission observation of interfaces. The research presented herein is the first step of this new approach in studying the corrosion with biological influence in pure copper.Commercially produced copper (Cu, 99%) foils of approximately 120 μm thick exposed to a copper-tolerant marine bacterium, Oceanospirillum, and an abiotic culture medium were subsampled (1 cm × 1 cm) for this study along with unexposed control samples.

Download Full-text

Reproduction and Organochlorine Contaminants in Terns at San Diego Bay

Colonial Waterbirds ◽

10.2307/1521193 ◽

1985 ◽

Vol 8 (1) ◽

pp. 42 ◽

Cited By ~ 17

Author(s):

Harry M. Ohlendorf ◽

Fred C. Schaffner ◽

Thomas W. Custer ◽

Charles J. Stafford

Keyword(s):

San Diego ◽

Organochlorine Contaminants ◽

San Diego Bay

Download Full-text

DNA damage in mussels at sites in San Diego Bay

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/s0027-5107(97)00267-4 ◽

1998 ◽

Vol 399 (1) ◽

pp. 65-85 ◽

Cited By ~ 107

Author(s):

Scott A Steinert ◽

Rebecca Streib-Montee ◽

James M Leather ◽

David B Chadwick

Keyword(s):

Dna Damage ◽

San Diego ◽

San Diego Bay

Download Full-text

DISTRIBUTION AND ABUNDANCE OF AEROBIC ANOXYGENIC PHOTOTROPHIC BACTERIA IN THE TROPICAL COASTAL WATERS OF GUNUNGKIDUL, YOGYAKARTA

KnE Life Sciences ◽

10.18502/kls.v2i1.150 ◽

2015 ◽

Vol 2 (1) ◽

pp. 244

Author(s):

Victor Aprilyanto ◽

Tjut Sugandawaty Djohan ◽

Langkah Sembiring

Keyword(s):

Coastal Waters ◽

Catchment Area ◽

Sampling Site ◽

Ammonium Phosphate ◽

Anoxygenic Phototrophic Bacteria ◽

Top Down ◽

Dissolved Nutrients ◽

Sampling Depth ◽

Coastal Fish ◽

Aerobic Anoxygenic Phototrophic Bacteria

This research was conducted to reveal the distribution and abundance of aerobic anoxygenic phototrophic (AAP) in the tropical coastal waters of Gunungkidul, Yogyakarta. Sampling site was determined at the coastal fish catchment area. We sampled and enumerated total bacterioplankton and AAP bacteria at four sampling depth which are 0, 4, 6, and 20 metre with five replicates each. Several dissolved nutrients such as nitrate, ammonium, phosphate, and sulfate in each respective depths were also measured. Several fluctuations in the nutrient distributions were observed and hypothesized as the regulating factors to the distribution and abundance of AAP bacteria as well as bacterioplankton. The results revealed that AAP bacteria were ranging from 3.83×102 –7.48×102 cell/ml, comprising about 1% to ~2.5% of total bacterioplankton. The abundance of both community were quite low and showed similar distributions with the nutrients. In conclusion, the distribution and abundance of AAP bacteria were regulated by nutrients as the bottom-up factors and also by predators such as nanoplanktons as the top-down factors. Keywords: tropical waters, microbial loop, predation.

Download Full-text