scholarly journals Diversity of Microorganisms within Rock Varnish in the Whipple Mountains, California

2006 ◽  
Vol 72 (2) ◽  
pp. 1708-1715 ◽  
Author(s):  
K. R. Kuhlman ◽  
W. G. Fusco ◽  
M. T. La Duc ◽  
L. B. Allenbach ◽  
C. L. Ball ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Microbial Community ◽  
Phospholipid Fatty Acids ◽  
Microbial Colonization ◽  
Rrna Gene ◽  
Rock Varnish ◽  
Gene Libraries ◽  
Whipple Mountains

ABSTRACT Rock varnish from Arizona's Whipple Mountains harbors a microbial community containing about 108 microorganisms g−1 of varnish. Analyses of varnish phospholipid fatty acids and rRNA gene libraries reveal a community comprised of mostly Proteobacteria but also including Actinobacteria, eukaryota, and a few members of the Archaea. Rock varnish represents a significant niche for microbial colonization.

scholarly journals The Microbial Community Structure in Petroleum-Contaminated Sediments Corresponds to Geophysical Signatures

2007 ◽  
Vol 73 (9) ◽  
pp. 2860-2870 ◽  
Author(s):  
Jonathan P. Allen ◽  
Estella A. Atekwana ◽  
Eliot A. Atekwana ◽  
Joseph W. Duris ◽  
D. Dale Werkema ◽  
...  
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Contaminated Sediments ◽  
Microbial Populations ◽  
Rrna Gene ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Gene Libraries ◽  
Cost Efficient

ABSTRACT The interdependence between geoelectrical signatures at underground petroleum plumes and the structures of subsurface microbial communities was investigated. For sediments contaminated with light non-aqueous-phase liquids, anomalous high conductivity values have been observed. Vertical changes in the geoelectrical properties of the sediments were concomitant with significant changes in the microbial community structures as determined by the construction and evaluation of 16S rRNA gene libraries. DNA sequencing of clones from four 16S rRNA gene libraries from different depths of a contaminated field site and two libraries from an uncontaminated background site revealed spatial heterogeneity in the microbial community structures. Correspondence analysis showed that the presence of distinct microbial populations, including the various hydrocarbon-degrading, syntrophic, sulfate-reducing, and dissimilatory-iron-reducing populations, was a contributing factor to the elevated geoelectrical measurements. Thus, through their growth and metabolic activities, microbial populations that have adapted to the use of petroleum as a carbon source can strongly influence their geophysical surroundings. Since changes in the geophysical properties of contaminated sediments parallel changes in the microbial community compositions, it is suggested that geoelectrical measurements can be a cost-efficient tool to guide microbiological sampling for microbial ecology studies during the monitoring of natural or engineered bioremediation processes.

Characterization of the rhizosphere microbial community from different Arabidopsis thaliana genotypes using phospholipid fatty acids (PLFA) analysis

2009 ◽  
Vol 329 (1-2) ◽  
pp. 315-325 ◽  
Author(s):  
Ana García-Villaraco Velasco ◽  
Agustin Probanza ◽  
F. Javier Gutierrez Mañero ◽  
Beatriz Ramos Solano ◽  
Jose Antonio Lucas
Keyword(s):  
Fatty Acids ◽  
Arabidopsis Thaliana ◽  
Microbial Community ◽  
Phospholipid Fatty Acids ◽  
Plfa Analysis ◽  
Rhizosphere Microbial Community

scholarly journals Gastrointestinal Segments Influenced Fermentation End-Products, Microbiota and Microbial Abundances in Goats

2021 ◽  
Author(s):  
Tsegay Gebremariam ◽  
Zhiliang Tan
Keyword(s):  
Fatty Acids ◽  
Microbial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Gene Copies ◽  
End Products ◽  
The Impact

Abstract Purpose: Carbohydrate diets altered fermentation end-products and microbial community in the gastrointestinal tracts (GIT) of goats. Gastrointestinal contents used to determine the impact of carbohydrate feeds on fermentation end-products and microbial community in goats.Methodology: in the study goats were assigned to one of the two treatments corn meal (CM) or Corn gluten (CG) in a randomized block design (400 g/kg DM each). Goats were slaughtered, GIT liquids were used to determine dissolved gasses, fatty acids and microbial community.Results: Goats fed CG increased molar acetate (P < 0.05), lowered butyrate and propionate in the fore and hindgut comparing to those goats received CM. Goats received CM had higher (P < 0.05) dH2 while lowered dH2S in the fore and hindgut than those goats fed with CG treatment. The fore and hindgut had higher (P < 0.01) 16S rRNA gene copies of bacteria, protozoa, methanogens and 18S rRNA gene copies fungi than in the ileum and cecum. Goats fed CG diet had higher (P < 0.05)16S rRNA gene copies of bacteria, protozoa, methanogens, and 18S rRNA gene copies of fungi than those goats fed with CM diet. Conclusion fore and hindguts improved dissolved gasses, fatty acids and microbial community comparing with in the ileum and cecum. Goats fed CM had improved the Methanobacterials order and Methanobrevibacter genus as compared with those goats fed CG. The study suggested that hindgut segments have a reasonable contribution as foregut to methane emissions from goats.

Microbial Diversity in a Pilot Plant for Producing Iron Hydroxysulfates

2007 ◽  
Vol 20-21 ◽  
pp. 527-530 ◽  
Author(s):  
Elke Heinzel ◽  
Sabrina Hedrich ◽  
Jana Seifert ◽  
Michael Schlömann
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Pilot Plant ◽  
Ferrous Iron ◽  
Sequence Type ◽  
Molecular Techniques ◽  
Rrna Gene ◽  
Mine Waters ◽  
Gene Libraries ◽  
The 16S Rrna Gene

The microbial community of a pilot plant for the production of iron hydroxysulfates by biological oxidation of ferrous iron was studied using molecular techniques. The 16S rRNA gene libraries were dominated by one sequence type, which can be classified phylogenetically to the class of Betaproteobacteria. These bacteria have no close cultivated relatives and were also proved as the dominating species in other mine waters. The microbial community was observed over a period of 18 months using TRFLP to investigate the influence of different process parameters on the composition of the microbial diversity. These studies verified the dominance of the Betaproteobacteria in the pilot plant.

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