scholarly journals Comparison of Three Antigenic Extracts of Eurotium amstelodami in Serological Diagnosis of Farmer's Lung Disease

2009 ◽  
Vol 17 (1) ◽  
pp. 160-167 ◽  
Author(s):  
Sandrine Roussel ◽  
Gabriel Reboux ◽  
Bénédicte Rognon ◽  
Michel Monod ◽  
Frédéric Grenouillet ◽  
...  
Keyword(s):  
Lung Disease ◽  
Culture Media ◽  
Area Under The Curve ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Characteristic Analysis ◽  
Farmer’S Lung ◽  
Farmer's Lung ◽  
Fungal Particles

ABSTRACT In France and Finland, farmer's lung disease (FLD), a hypersensitivity pneumonitis common in agricultural areas, is mainly caused by Eurotium species. The presence of antibodies in patients' serum is an important criterion for diagnosis. Our study aimed to improve the serological diagnosis of FLD by using common fungal particles that pollute the farm environment as antigens. Fungal particles of the Eurotium species were observed in handled hay. A strain of Eurotium amstelodami was grown in vitro using selected culture media; and antigen extracts from sexual (ascospores), asexual (conidia), and vegetative (hyphae) forms were made. Antigens were tested by enzyme-linked immunosorbent assay (ELISA), which was used to test for immunoglobulin G antibodies from the sera of 17 FLD patients, 40 healthy exposed farmers, and 20 nonexposed controls. The antigens were compared by receiver operating characteristic analysis, and a threshold was then established. The ascospores contained in asci enclosed within cleistothecia were present in 38% of the hay blades observed; conidial heads of aspergillus were less prevalent. The same protocol was followed to make the three antigen extracts. A comparison of the results for FLD patients and exposed controls showed the area under the curve to be 0.850 for the ascospore antigen, 0.731 for the conidia, and 0.690 for the hyphae. The cutoffs that we determined, with the standard deviation for measures being taken into account, showed 67% for sensitivity and 92% for specificity with the ascospore antigen. In conclusion, the serological diagnosis of FLD by ELISA was improved by the adjunction of ascospore antigen.

scholarly journals Anti-UBE2T antibody: a novel biomarker of progressive-fibrosing interstitial lung disease

2021 ◽  
Author(s):  
Mari Hikichi ◽  
Yasuhiro Gon ◽  
Kenji Mizumura ◽  
Shu Hashimoto ◽  
M.D. M Shuichiro Ph.D.
Keyword(s):  
Interstitial Lung Disease ◽  
Lung Disease ◽  
Protein Microarray ◽  
Area Under The Curve ◽  
Enzyme Linked Immunosorbent Assay ◽  
Characteristic Analysis ◽  
Honeycomb Structures ◽  
Antifibrotic Therapy ◽  
Circulating Autoantibodies ◽  
Sensitivity Specificity

Abstract Background Antifibrotic therapy has demonstrated efficacy against progressive-fibrosing interstitial lung disease (PF-ILD); therefore, it has become a priority to identify disease behavior before disease presentation. As autoimmunity is implicated in the pathogenesis of various ILDs, we explored the possibility of a circulating biomarker that can predict the chronic progressive behavior of ILDs. Methods A single-center retrospective cohort study was conducted to investigate a biomarker of PF-ILD. Circulating autoantibodies against 9,483 purified full-length human recombinant proteins of patients with interstitial pneumonia were screened by microarray analysis. The candidate auto-antibodies were verified their existence by multiples solution assay. In addition, enzyme-linked immunosorbent assay (ELISA) was performed in larger sample sets to evaluate accurate sensitivity, specificity and clinical significance in ILDs. Results In total, 61 healthy subjects and 87 patients with various ILDs enrolled in this study. Anti-UBE2T antibody was discovered by performing protein microarray and multiplex solution assay as a candidate biomarker of ILDs. By measuring its concentration by ELISA, anti-ubiquitin-conjugating enzyme E2T (UBE2T) antibody levels were significantly higher in patients with idiopathic interstitial pneumonias (IIPs), especially in those with PF-ILDs, than in healthy participants. The receiver operating characteristic analysis of anti-UBE2T antibody in diagnosing PF-ILD was calculated. The area under the curve was 0.85 and yielded a cut-off value of 238.1 ng/mL. Anti-UBE2T antibody-positive IIP patients demonstrated significantly higher ILD-gender age physiology scores, PF-ILD diagnosis rates and were more likely to develop honeycomb structures than anti-UBE2T-negative IIP patients after two years of follow up. The anti-UBE2T antibody positivity did not correlate with other commercial biomarkers such as KL-6 and commercial autoantibodies, suggesting the presence of anti-UBE2T antibody was independent of the others. Immunohistochemical staining of UBE2T in normal lungs was observed sparsely in the bronchiole epithelium and macrophages. Controversially, idiopathic pulmonary fibrosis lung tissue showed robust expression of the UBE2T protein in the lining epithelium of honeycomb structures. Conclusion This is the first report to describe anti-UBE2T antibody, a new biomarker that is significantly elevated in idiopathic PF-ILDs. This new antibody may constitute a sensitive biomarker to detect cases of PF-ILDs that are not currently detected by commercially available biomarkers.

scholarly journals Antibody responses in patients with farmer's lung disease to antigens fromThermoactinomyces vulgaris

1975 ◽  
Vol 74 (1) ◽  
pp. 35-41 ◽  
Author(s):  
M. R. Hollingdale
Keyword(s):  
Lung Disease ◽  
Sodium Periodate ◽  
Latex Agglutination ◽  
Antibody Responses ◽  
Muramic Acid ◽  
Farmer’S Lung ◽  
Farmer's Lung ◽  
Human Sera ◽  
Thermoactinomyces Vulgaris ◽  
Positive Results

SUMMARYA serological analysis of mycelial antigens ofThermoactinomyces vulgarisin immunodiffusion with human sera revealed five individual antigens. Three antigens were proteins, sensitive to pronase and soluble in phenol. Two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid.Latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies toT. vulgaris;the number of positive results detected by each test differed slightly, and a combination of the two tests detected the highest number. Counterimmunoelectrophoresis (CIE) was shown to be a very sensitive method for detecting precipitins, but not for their measurement. A prospective evaluation of immunodiffusion, latex agglutination and CIE as potential serodiagnostic techniques for farmer's lung disease is suggested.

IgG and IgA subclass antibodies against Aspergillus umbrosus in farmer's lung disease

1993 ◽  
Vol 23 (10) ◽  
pp. 851-856 ◽  
Author(s):  
K. KAUKONEN ◽  
J. SAVOLAINEN ◽  
M. VIANDER ◽  
M. KOTIMAA ◽  
E. O. TERHO
Keyword(s):  
Lung Disease ◽  
Farmer’S Lung ◽  
Farmer's Lung

New Proof Of The Involvement Of Absidia Corymbifera In Farmer’s Lung Disease

2010 ◽  
Author(s):  
Anne-Pauline Bellanger ◽  
Gabriel Reboux ◽  
Françoise Botterel ◽  
charline Candido ◽  
Sandrine Roussel ◽  
...  
Keyword(s):  
Lung Disease ◽  
Absidia Corymbifera ◽  
Farmer’S Lung ◽  
Farmer's Lung

scholarly journals Sensitive Blood-Based Detection of Asbestos-Associated Diseases Using Cysteine-Rich Angiogenic Inducer 61 as Circulating Protein Biomarker

2020 ◽  
Author(s):  
Kai Bartkowiak ◽  
Swaantje Casjens ◽  
Antje Andreas ◽  
Lucija Ačkar ◽  
Simon A Joosse ◽  
...  
Keyword(s):  
Large Scale ◽  
Mononuclear Cells ◽  
Area Under The Curve ◽  
Immunofluorescent Staining ◽  
Youden Index ◽  
Enzyme Linked Immunosorbent Assay ◽  
Characteristic Analysis ◽  
Clinical Sensitivity ◽  
Associated Diseases ◽  
Healthy Control

Abstract Background Detection of asbestos-associated diseases like asbestosis or mesothelioma is still challenging. We sought to improve the diagnosis of benign asbestos-associated disease (BAAD) by detection of the protein cysteine-rich angiogenic inducer 61 (Cyr61) in human plasma. Methods Plasma Cyr61 was quantified using an enzyme-linked immunosorbent assay. Plasma samples from males diagnosed with BAAD, but without a malignant disease (n = 101), and malignant mesothelioma (n = 21; 15 males, 6 females), as well as nonasbestos-exposed healthy control participants (n = 150; 58 males, 92 females) were analyzed. Clinical sensitivity and specificity of Cyr61 were determined by receiver operating characteristic analysis. Results The median plasma Cyr61 concentration for healthy control participants was 0.27 ng/mL. Cytoplasmic Cyr61 in peripheral blood mononuclear cells from healthy control participants was evenly distributed, as detected by immunofluorescent staining. The increase in plasma Cyr61 concentrations in the BAAD study group was statistically significant compared to the healthy control participants (P < 0.0001). For the detection of BAAD vs male healthy control participants, clinical sensitivity was 88% and clinical specificity 95% with an area under the curve of 0.924 at maximal Youden Index. For a predefined clinical specificity of 100%, the clinical sensitivity was 76%. For male mesothelioma patients vs male healthy control participants, the clinical sensitivity at maximal Youden Index was 95% with a clinical specificity of 100% (area under the curve, 0.997) and for a predefined clinical specificity of 100%, the clinical sensitivity was 93%. Conclusions In our study, plasma Cyr61 protein concentrations showed to be a new biomarker for asbestos-associated diseases like BAAD and mesothelioma in men, which deserves further investigation in large-scale cohort studies.

ULTRASTRUCTURAL STUDY OF BRONCHOPULMONARY LAVAGE LIQUID IN FARMER'S LUNG DISEASE

The Lancet ◽  
1980 ◽  
Vol 315 (8171) ◽  
pp. 777 ◽  
Author(s):  
J.L. Romet-Lemonne ◽  
E. Lemarie ◽  
P. Choutet
Keyword(s):  
Lung Disease ◽  
Ultrastructural Study ◽  
Farmer’S Lung ◽  
Farmer's Lung
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