scholarly journals Naturally Occurring Anti-Escherichia coli Protein Antibodies in the Sera of Healthy Humans Cause Analytical Interference in a Recombinant Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Severe Acute Respiratory Syndrome

2006 ◽  
Vol 14 (1) ◽  
pp. 99-101 ◽  
Author(s):  
Chi Wai Yip ◽  
Chung Chau Hon ◽  
Fanya Zeng ◽  
Ken Y. C. Chow ◽  
Kwok Hung Chan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Severe Acute Respiratory Syndrome ◽  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
False Positives ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Humans ◽  
Analytical Interference ◽  
Human Sera ◽  
Recombinant Nucleocapsid Protein

ABSTRACT We reported the analytical interference of anti-Escherichia coli protein (EP) antibodies in human sera and residual EP in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay as a possible source of false positives in severe acute respiratory syndrome serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.

scholarly journals Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS

2005 ◽  
Vol 125 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Masayuki Saijo ◽  
Toshio Ogino ◽  
Fumihiro Taguchi ◽  
Shuetsu Fukushi ◽  
Tetsuya Mizutani ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Nucleocapsid Protein

Evaluation of an Enzyme-Linked Immunosorbent Assay Based on Araraquara Virus Recombinant Nucleocapsid Protein

2009 ◽  
Vol 81 (2) ◽  
pp. 273-276 ◽  
Author(s):  
Luiz Tadeu Moraes Figueiredo ◽  
Glauciane Garcia de Figueiredo ◽  
Ivani Bisordi ◽  
Alessandra Abel Borges ◽  
Paula Padula ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Recombinant ◽  
Recombinant Nucleocapsid Protein ◽  
Araraquara Virus

scholarly journals Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus

2005 ◽  
Vol 12 (3) ◽  
pp. 474-476 ◽  
Author(s):  
Maofeng Qiu ◽  
Jin Wang ◽  
Hongxia Wang ◽  
Zeliang Chen ◽  
Erhei Dai ◽  
...  
Keyword(s):  
Amino Acids ◽  
Severe Acute Respiratory Syndrome ◽  
Epidemiological Study ◽  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sars Coronavirus ◽  
Specific Test ◽  
N Protein

ABSTRACT Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.

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