scholarly journals Development of a Fluorescent-Bead-Based Multiplex Immunoassay To Determine Immunoglobulin G Subclass Responses to Neisseria meningitidis Serogroup A and C Polysaccharides

2008 ◽  
Vol 15 (8) ◽  
pp. 1188-1193 ◽  
Author(s):  
Richarda M. de Voer ◽  
Fiona R. M. van der Klis ◽  
Carla W. A. M. Engels ◽  
Ger T. Rijkers ◽  
Elisabeth A. Sanders ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Fluorescent Particle ◽  
Multiplex Immunoassay ◽  
Specific Assay ◽  
Flow Cytometric ◽  
Immunoglobulin G Subclass

ABSTRACT A fluorescent-particle-based multiplex flow cytometric immunoassay (MIA) for the detection of serum immunoglobulin G (IgG) and two IgG subclasses, IgG1 and IgG2, specific for Neisseria meningitidis serogroup A (MenA) and C (MenC) polysaccharides (PS) was developed. The assay comprised three separate duplex assays, one for the detection of the IgG response to MenA and MenC PS, another for the detection of the IgG1 response to MenA and MenC PS, and a third for the detection of the IgG2 response to MenA and MenC PS. Next, the three separate duplex assays were combined and analyzed as a hexaplex assay. No interference between monoplex, duplex, and hexaplex assays was observed, and the assay was found to have low intra- and interassay variation (<9.0% and <27%, respectively). Comparison of the meningococcal subclass MIA to the in-house enzyme-linked inmmunosorbent assays showed a good correlation (R ≥ 0.85) for each of the subclasses. We conclude that the hexaplex meningococcal subclass MIA is an easy and specific assay for the determination of anti-MenA and anti-MenC PS subclass IgG, requiring minimal amounts of serum to study IgG subclass responses to vaccines.

scholarly journals Differential Patterns of Human Immunoglobulin G Subclass Responses to Distinct Regions of a Single Protein, the Merozoite Surface Protein 1 of Plasmodium falciparum

2001 ◽  
Vol 69 (2) ◽  
pp. 1207-1211 ◽  
Author(s):  
David R. Cavanagh ◽  
Carlota Dobaño ◽  
Ibrahim M. Elhassan ◽  
Kevin Marsh ◽  
Ahmed Elhassan ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunoglobulin G ◽  
Malaria Infection ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Igg Subclass ◽  
Polymorphic Region ◽  
Merozoite Surface Protein 1 ◽  
Single Protein ◽  
Immunoglobulin G Subclass

ABSTRACT Comparisons of immunoglobulin G (IgG) subclass responses to the major polymorphic region and to a conserved region of MSP-1 in three cohorts of African villagers exposed to Plasmodium falciparum revealed that responses to Block 2 are predominantly IgG3 whereas antibodies to MSP-119 are mainly IgG1. The striking dominance of IgG3 to Block 2 may explain the short duration of this response and also the requirement for continuous stimulation by malaria infection to maintain clinical immunity.

Serum immunoglobulin G subclass responses in different phases of hepatitis E virus infection

2013 ◽  
Vol 85 (5) ◽  
pp. 828-832 ◽  
Author(s):  
Tejaswini M. Deshmukh ◽  
Rachita R. Shah ◽  
Yogesh K. Gurav ◽  
Vidya A. Arankalle
Keyword(s):  
Virus Infection ◽  
Immunoglobulin G ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Serum Immunoglobulin ◽  
Immunoglobulin G Subclass

Evaluation of a Test Kit for Determination of Serum Immunoglobulin G Concentration in Foals

1988 ◽  
Vol 2 (4) ◽  
pp. 181-183 ◽  
Author(s):  
Joseph J. Bertone ◽  
Robert L. Jones ◽  
Charles R. Curtis
Keyword(s):  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Test Kit

Serum immunoglobulin G subclass levels and estimated clinical severity caused by possible influenza A (H1N1) pdm 2009 infection

2013 ◽  
Vol 19 (5) ◽  
pp. 833-842 ◽  
Author(s):  
Takehisa Yamamoto ◽  
Yoshimi Mizoguchi ◽  
Hiroshi Kaneno ◽  
Katsusuke Yamamoto ◽  
Tsunesuke Shimotsuji ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Influenza A ◽  
Clinical Severity ◽  
Serum Immunoglobulin ◽  
Influenza A H1n1 ◽  
Immunoglobulin G Subclass

scholarly journals Immunoglobulin G (IgG) Subclass and IgE Responses in Human Paragonimiases Caused by Three Different Species

1998 ◽  
Vol 5 (4) ◽  
pp. 474-478 ◽  
Author(s):  
Yoon Kong ◽  
Akira Ito ◽  
Hyun-Jong Yang ◽  
Young-Bae Chung ◽  
Shiro Kasuya ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Positive Reaction ◽  
Immunoglobulin G ◽  
Igg Subclasses ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Healthy Controls ◽  
Paragonimus Westermani ◽  
Egg Extracts ◽  
Specific Reactions

ABSTRACT In 40 cases of human paragonimiases caused by Paragonimus westermani (20 cases), P. miyazakii (10 cases), andP. skrjabini (10 cases), responses of serum immunoglobulin G (IgG), IgG subclasses, and IgE were analyzed by immunoblotting with crude antigens prepared from egg, 4-week-old juvenile, and adult forms of P. westermani. The 32- and 35-kDa proteins in the adult extracts showed specific reactions regardless of the causative species (39 of 40 cases; 98%). Sera of patients infected with P. westermani and P. miyazakii reacted strongly with the 28-, 46-, and 94-kDa proteins of egg extracts, while those from patients infected with P. skrjabini reacted faintly. No sera from patients with other trematodiases (0 of 15 cases), cestodiases (0 of 20 cases), or lung cancer (0 of 5 cases) or from healthy controls (0 of 10 individuals) showed positive reactions. Analysis by IgG subclass revealed that IgG4 (33 of 40 cases; 83%) and IgG1 (29 of 40 cases; 73%) antibodies in the patient sera recognized the 32- and 35-kDa proteins predominantly. IgG3 reaction was found in 50% (10 of 20 cases) and 30% (3 of 10 cases) of the sera of patients infected with P. westermani and P. miyazakii, respectively. In an IgE immunoblot, 83% (33 of 40 cases) of the sera from paragonimiasis patients reacted with the 32- and 35-kDa proteins while no sera from patients with heterologous diseases and healthy controls showed a positive reaction. Both 32- and 35-kDa proteins in adult extracts of P. westermani were highly reliable for serodiagnosis of human paragonimiases.

Papillon-Lefèvre Syndrome: Serum Immunoglobulin G (IgG) Subclass Antibody Response to Periodontopathic Bacteria. A Case Report

2001 ◽  
Vol 72 (12) ◽  
pp. 1747-1754 ◽  
Author(s):  
Nawarat Wara-aswapati ◽  
Jinda Lertsirivorakul ◽  
Toshiyuki Nagasawa ◽  
Yoko Kawashima ◽  
Isao Ishikawa
Keyword(s):  
Case Report ◽  
Antibody Response ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Periodontopathic Bacteria
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