scholarly journals Immunoglobulin G (IgG) Subclass and IgE Responses in Human Paragonimiases Caused by Three Different Species

1998 ◽  
Vol 5 (4) ◽  
pp. 474-478 ◽  
Author(s):  
Yoon Kong ◽  
Akira Ito ◽  
Hyun-Jong Yang ◽  
Young-Bae Chung ◽  
Shiro Kasuya ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Positive Reaction ◽  
Immunoglobulin G ◽  
Igg Subclasses ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Healthy Controls ◽  
Paragonimus Westermani ◽  
Egg Extracts ◽  
Specific Reactions

ABSTRACT In 40 cases of human paragonimiases caused by Paragonimus westermani (20 cases), P. miyazakii (10 cases), andP. skrjabini (10 cases), responses of serum immunoglobulin G (IgG), IgG subclasses, and IgE were analyzed by immunoblotting with crude antigens prepared from egg, 4-week-old juvenile, and adult forms of P. westermani. The 32- and 35-kDa proteins in the adult extracts showed specific reactions regardless of the causative species (39 of 40 cases; 98%). Sera of patients infected with P. westermani and P. miyazakii reacted strongly with the 28-, 46-, and 94-kDa proteins of egg extracts, while those from patients infected with P. skrjabini reacted faintly. No sera from patients with other trematodiases (0 of 15 cases), cestodiases (0 of 20 cases), or lung cancer (0 of 5 cases) or from healthy controls (0 of 10 individuals) showed positive reactions. Analysis by IgG subclass revealed that IgG4 (33 of 40 cases; 83%) and IgG1 (29 of 40 cases; 73%) antibodies in the patient sera recognized the 32- and 35-kDa proteins predominantly. IgG3 reaction was found in 50% (10 of 20 cases) and 30% (3 of 10 cases) of the sera of patients infected with P. westermani and P. miyazakii, respectively. In an IgE immunoblot, 83% (33 of 40 cases) of the sera from paragonimiasis patients reacted with the 32- and 35-kDa proteins while no sera from patients with heterologous diseases and healthy controls showed a positive reaction. Both 32- and 35-kDa proteins in adult extracts of P. westermani were highly reliable for serodiagnosis of human paragonimiases.

scholarly journals Biased Immunoglobulin G (IgG) Subclass Production in a Case of Hyper-IgM Syndrome

2004 ◽  
Vol 11 (6) ◽  
pp. 1192-1193 ◽  
Author(s):  
G. R. McLean ◽  
K. K. Miller ◽  
J. W. Schrader ◽  
A. K. Junker
Keyword(s):  
Immune Deficiency ◽  
Immunoglobulin G ◽  
Somatic Hypermutation ◽  
Igg Subclasses ◽  
Immunoglobulin M ◽  
Primary Immune Deficiency ◽  
Igg Subclass ◽  
Hyper Igm Syndrome ◽  
Hyper Igm ◽  
V Region

ABSTRACT Hyper-immunoglobulin M (IgM) syndrome (HIGM) is a rare heterogeneous primary immune deficiency. We describe a patient with HIGM characterized by skewed production of serum IgG subclasses and normal somatic hypermutation. This case may represent a subgroup of HIGM type 4 that is characterized by a biased switching to the V-region proximal constant regions.

scholarly journals Development of a Fluorescent-Bead-Based Multiplex Immunoassay To Determine Immunoglobulin G Subclass Responses to Neisseria meningitidis Serogroup A and C Polysaccharides

2008 ◽  
Vol 15 (8) ◽  
pp. 1188-1193 ◽  
Author(s):  
Richarda M. de Voer ◽  
Fiona R. M. van der Klis ◽  
Carla W. A. M. Engels ◽  
Ger T. Rijkers ◽  
Elisabeth A. Sanders ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Fluorescent Particle ◽  
Multiplex Immunoassay ◽  
Specific Assay ◽  
Flow Cytometric ◽  
Immunoglobulin G Subclass

ABSTRACT A fluorescent-particle-based multiplex flow cytometric immunoassay (MIA) for the detection of serum immunoglobulin G (IgG) and two IgG subclasses, IgG1 and IgG2, specific for Neisseria meningitidis serogroup A (MenA) and C (MenC) polysaccharides (PS) was developed. The assay comprised three separate duplex assays, one for the detection of the IgG response to MenA and MenC PS, another for the detection of the IgG1 response to MenA and MenC PS, and a third for the detection of the IgG2 response to MenA and MenC PS. Next, the three separate duplex assays were combined and analyzed as a hexaplex assay. No interference between monoplex, duplex, and hexaplex assays was observed, and the assay was found to have low intra- and interassay variation (<9.0% and <27%, respectively). Comparison of the meningococcal subclass MIA to the in-house enzyme-linked inmmunosorbent assays showed a good correlation (R ≥ 0.85) for each of the subclasses. We conclude that the hexaplex meningococcal subclass MIA is an easy and specific assay for the determination of anti-MenA and anti-MenC PS subclass IgG, requiring minimal amounts of serum to study IgG subclass responses to vaccines.

Immunoglobulin G (IgG) and IgG subclass reference intervals in children, using Optilite® reagents

2018 ◽  
Vol 56 (8) ◽  
pp. 1319-1327 ◽  
Author(s):  
Olivier Grunewald ◽  
Benjamin Lopez ◽  
Séverine Brabant ◽  
Stéphanie Rogeau ◽  
Antoine Deschildre ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Age Groups ◽  
Reference Intervals ◽  
Igg Subclasses ◽  
Specific Reference ◽  
Igg Subclass ◽  
Serum Samples ◽  
Gender Specific Differences ◽  
Specific Igg ◽  
Changes Over Time

Abstract Background: Immunoglobulin G (IgG) and IgG subclass assays are indicated in patients with suspected primary immunodeficiency (PID). Commercially available assays for IgG subclass determination are calibrated against various preparations, and so specific reference values are required for each of them. Using Optilite® reagents from The Binding Site Group Ltd., we sought to determine the pediatric IgG and IgG subclass reference intervals with respect to the ERM-DA470k certified reference material. Methods: Levels of IgG and IgG subclasses were analyzed in serum samples collected from a large cohort of PID-free children and adolescents. Reference intervals were calculated for previously published age groups (6–12 months, 12–18 months, 18 months–2 years, 2–3 years, 3–4 years, 4–6 years, 6–9 years, 9–12 years and 12–18 years), according to the Clinical and Laboratory Standards Institute’s C28-A3c protocol. Results: A total of 456 serum samples were analyzed. The correlation between the total IgG and the sum of the IgG subclasses was good (r2=0.96). No statistically significant gender-specific differences were observed. Our results for the changes over time in IgG and IgG subclass levels are consistent with previous reports. The differences between our lower/upper reference limits and those in the literature are probably due to variations in calibration. Conclusions: Our present results provide a reliable basis for the diagnosis of PIDs in childhood and for the accreditation of laboratories using Optilite® immunoturbidimetric reagents for IgG subclass measurement. Laboratory scientists and clinicians should be aware of the need for manufacturer-specific IgG subclass reference intervals.

Distribution of IgG subclass anti-nuclear antibodies (ANAs) in systemic lupus erythematosus

Lupus ◽  
2021 ◽  
Vol 30 (6) ◽  
pp. 901-912 ◽  
Author(s):  
Yanli Zeng ◽  
Yan Zhang ◽  
Qinggui Chen ◽  
Qinghe Huang ◽  
Yiqiang Lin ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Serum Level ◽  
Lupus Erythematosus ◽  
Inflammatory Process ◽  
Igg Subclasses ◽  
Diagnostic Tools ◽  
High Avidity ◽  
Igg Subclass ◽  
Healthy Controls ◽  
Systemic Lupus

Objectives Our study purpose was to detect the distribution of anti-nuclear antibody (ANA) IgG subclasses in patients with systemic lupus erythematosus (SLE) and to evaluate their influence on the inflammatory process in SLE. Methods We determined the serum levels of ANA IgG subclasses from 70 SLE patients, 25 patients with other autoimmune diseases (OAD), and 25 healthy controls using ELISA. The serum level of total ANA IgG and the avidity of ANA IgG, dsDNA IgG, and dsDNA IgG subclasses were analysed by ELISA. Results The results indicated that levels of four ANA IgG subclasses (IgG1, IgG2, IgG3 and IgG4) and total IgG were significantly higher in SLE patients than in OAD patients and healthy controls ( p < 0.001). Moreover, the level of each ANA IgG subclass and the prevalence of high-avidity IgG ANAs (HA IgG ANAs) were significantly higher in the active cases than in the inactive cases of SLE and LN. Furthermore, level of ANA IgG subclasses decreased as level of dsDNA IgG subclasses decreased in 30 patients with SLE. In comparison, ANA IgG3 was significantly effective in high-dose prednisone combined with hydroxychloroquine ( p = 0.025). Additionally, it revealed that level of dsDNA IgG had a significant influence on four ANA IgG subclasses, especially on ANA IgG3 (β coefficient = 0.649, p < 0.001). Level of ANA IgG3 was also positively related to the serum level of dsDNA IgG (r = 0.729, p < 0.001) and RAI of HA IgG ANAs (r = 0.504, p < 0.001). However, the level of ANA IgG4 was positively related to the serum level of albumin (r = 0.572, p < 0.001) and RAI of HA IgG ANAs (r = 0.549, p < 0.001). Moreover, the results revealed that cutaneous and renal involvement were mainly associated with the ANA IgG1 and IgG4 subclasses. Although, arthritic involvement was mainly associated with ANA IgG3. Conclusions First, we demonstrated that the ANA IgG subclasses were diagnostic tools in SLE patients. Furthermore, HA IgG ANAs might affect the distribution of ANA IgG3 and IgG4. Moreover, ANA IgG3 might play a particular role in the activity of SLE disease and therapy. Therefore, an altered ANA IgG subclass distribution might be a risk factor influencing the inflammatory process in SLE.

scholarly journals Immunoglobulin G (IgG) Subclass Distribution and IgG1 Avidity of Antibodies in Human Immunodeficiency Virus-Infected Individuals after Revaccination with Tetanus Toxoid

1999 ◽  
Vol 6 (3) ◽  
pp. 352-355 ◽  
Author(s):  
F. P. Kroon ◽  
M. J. D. van Tol ◽  
C. M. Jol-van der Zijde ◽  
R. van Furth ◽  
J. T. van Dissel
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Lymphocyte ◽  
Tetanus Toxoid ◽  
Immunoglobulin G ◽  
Igg Subclass ◽  
Healthy Controls ◽  
Group I ◽  
Immunodeficiency Virus ◽  
I 11 ◽  
Group Ii

ABSTRACT In human immunodeficiency virus (HIV)-infected individuals the amount of antibodies formed after vaccination with T-cell-dependent recall antigens such as tetanus toxoid is proportional to the peripheral blood CD4+ T-lymphocyte counts. To investigate whether the immunoglobulin G (IgG) subclass distribution and avidity of the antibodies produced after vaccination are affected as well, we gave 13 HIV-infected adults with low CD4+ T-lymphocyte counts (<200 × 106/liter; group I), 11 HIV-infected adults with intermediate CD4+ T-lymphocyte counts (≥200 × 106/liter; group II), and 5 healthy controls booster immunizations with tetanus toxoid. The prevaccination antibody concentrations against tetanus toxoid were similar in the HIV-infected and healthy adults. After vaccination the total IgG and the IgG1 anti-tetanus toxoid antibody concentrations were significantly lower in group I than in group II and the controls. The avidity of the IgG1 anti-tetanus toxoid antibodies formed by HIV-infected adults was within the range for healthy controls, irrespective of their CD4+T-lymphocyte counts.

scholarly journals Characterization of Immunoglobulin G and Its Subclass Response to Indian Kala-Azar Infection before and after Chemotherapy

2004 ◽  
Vol 72 (2) ◽  
pp. 863-870 ◽  
Author(s):  
Rajesh Ravindran ◽  
Khairul Anam ◽  
Bibhas C. Bairagi ◽  
Bibhuti Saha ◽  
Netai Pramanik ◽  
...  
Keyword(s):  
Western Blot ◽  
Immunoglobulin G ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Igg Subclasses ◽  
Cross Reaction ◽  
Future Application ◽  
Healthy Controls ◽  
Serum Samples ◽  
Kala Azar

ABSTRACT Serologic parameters of kala-azar were evaluated by Western blot analysis. Sera from kala-azar patients with confirmed diagnoses were screened for immunoglobulin G (IgG) and IgG subclass-specific reactivity against Leishmania donovani membrane antigen (LAg). Heterogenous LAg-specific IgG reactivity with numerous proteins with molecular masses ranging from 18 to 190 kDa was observed. Though the individual band patterns were varied, seven polypeptides of approximately 31, 34, 51, 63, 72, 91, and 120 kDa were immunoreactive with all the sera tested from kala-azar patients. The band patterns of the immunoblots of sera from patients after treatment and clinical cure with sodium antimony gluconate revealed a decrease in the frequency of the bands. Still, recognition of the 63- and 120-kDa bands was 100%, and the 55- and 91-kDa fractions were recognized in 93% of the sera from cured individuals. Among the IgG subclasses, IgG1 reacted with the greatest number of polypeptides. The 63-kDa protein was again detected by all of the IgG subclasses of all the sera tested. Other fractions recognized by the subclasses of more than 70% of the serum samples included those of 47, 51, 55, and 78 kDa. Following treatment, 63- and 51-kDa bands were the most reactive with the IgG subclasses. LAg-associated cross-reaction with other reference human antisera revealed a mild reactivity of the 63-kDa polypeptide with some of the serum samples from leprosy, malaria, typhoid, tuberculosis, and healthy controls. Western blot analysis of LAg entrapped in liposomes, strong vaccine candidates against experimental visceral leishmaniasis, revealed a more restricted band pattern. The 63-kDa fraction revealed by all pre- and posttreatment sera showed almost negligible levels of cross-reaction with sera from patients with other diseases or from healthy controls. These observations provide insight into induced immunity during kala-azar infection for future application.

scholarly journals Differential Decline in Leishmania Membrane Antigen-Specific Immunoglobulin G (IgG), IgM, IgE, and IgG Subclass Antibodies in Indian Kala-Azar Patients after Chemotherapy

1999 ◽  
Vol 67 (12) ◽  
pp. 6663-6669 ◽  
Author(s):  
Khairul Anam ◽  
Farhat Afrin ◽  
Dwijadas Banerjee ◽  
Netai Pramanik ◽  
Subhasis K. Guha ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Immunoglobulin G ◽  
Fold Increase ◽  
Igg Subclasses ◽  
Igg Subclass ◽  
Kala Azar ◽  
Specific Immunoglobulin ◽  
Strong Stimulation ◽  
Specific Igg ◽  
Stimulation Of

ABSTRACT Pathogenesis in kala-azar is associated with depressed cellular immunity and significant elevation of antileishmanial antibodies. Since these antibodies are present even after cure, analysis of the parasite-specific isotypes and immunoglobulin G (IgG) subclasses in kala-azar patients may shed new light on the immune responses during progression and resolution of infection. Using leishmanial membrane antigenic extracts, we investigated the relative levels of specific IgG, IgM, IgA, IgE, and IgG subclasses in Indian kala-azar patient sera during disease, drug resistance, and cure. Acute-phase sera showed strong stimulation of IgG, followed by IgE and IgM and lastly by IgA antibodies. IgG subclass analysis revealed expression of all of the subclasses, with a predominance of IgG1 during disease. Following sodium stibogluconate (SAG) resistance, the levels of IgG, IgM, IgE, and IgG4 remained constant, while there was a decrease in the titers of IgG2 and IgG3. In contrast, a significant (2.2-fold) increase in IgG1 was observed in these individuals. Cure, in both SAG-responsive and unresponsive patients, correlated with a decline in the levels of IgG, IgM, IgE, and all of the IgG subclasses. The stimulation of IgG1 and the persistence, most importantly, of IgE and IgG4 following drug resistance, along with a decline in IgE, IgG4, and IgG1 with cure, demonstrate the potential of these isotypes as possible markers for monitoring effective treatment in kala-azar.

Papillon-Lefèvre Syndrome: Serum Immunoglobulin G (IgG) Subclass Antibody Response to Periodontopathic Bacteria. A Case Report

2001 ◽  
Vol 72 (12) ◽  
pp. 1747-1754 ◽  
Author(s):  
Nawarat Wara-aswapati ◽  
Jinda Lertsirivorakul ◽  
Toshiyuki Nagasawa ◽  
Yoko Kawashima ◽  
Isao Ishikawa
Keyword(s):  
Case Report ◽  
Antibody Response ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Periodontopathic Bacteria
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