scholarly journals The L-Type Calcium Ion Channel Cch1 Affects Ascospore Discharge and Mycelial Growth in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum)

2007 ◽  
Vol 7 (2) ◽  
pp. 415-424 ◽  
Author(s):  
Heather E. Hallen ◽  
Frances Trail
Keyword(s):  
Ion Channel ◽  
Calcium Ion ◽  
Mycelial Growth ◽  
Transcript Abundance ◽  
Gene Replacement ◽  
Gibberella Zeae ◽  
Transcriptional Analysis ◽  
Saprobic Fungi ◽  
Ascospore Discharge ◽  
Ascospore Development

ABSTRACT Cch1, a putative voltage-gated calcium ion channel, was investigated for its role in ascus development in Gibberella zeae. Gene replacement mutants of CCH1 were generated and found to have asci which did not forcibly discharge spores, although morphologically ascus and ascospore development in the majority of asci appeared normal. Additionally, mycelial growth was significantly slower, and sexual development was slightly delayed in the mutant; mutant mycelia showed a distinctive fluffy morphology, and no cirrhi were produced. Wheat infected with Δcch1 mutants developed symptoms comparable to wheat infected with the wild type; however, the mutants showed a reduced ability to protect the infected stalk from colonization by saprobic fungi. Transcriptional analysis of gene expression in mutants using the Affymetrix Fusarium microarray showed 2,449 genes with significant, twofold or greater, changes in transcript abundance across a developmental series. This work extends the role of CCH1 to forcible spore discharge in G. zeae and suggests that this channel has subtle effects on growth and development.

scholarly journals Mid1, a Mechanosensitive Calcium Ion Channel, Affects Growth, Development, and Ascospore Discharge in the Filamentous Fungus Gibberella zeae

2011 ◽  
Vol 10 (6) ◽  
pp. 832-841 ◽  
Author(s):  
Brad Cavinder ◽  
Ahmed Hamam ◽  
Roger R. Lew ◽  
Frances Trail
Keyword(s):  
Ion Channel ◽  
Calcium Ion ◽  
Vegetative Growth ◽  
Gibberella Zeae ◽  
Calcium Flux ◽  
Wild Type ◽  
Content Type ◽  
Ascospore Discharge ◽  
Ascospore Development

ABSTRACT The role of Mid1, a stretch-activated ion channel capable of being permeated by calcium, in ascospore development and forcible discharge from asci was examined in the pathogenic fungus Gibberella zeae (anamorph Fusarium graminearum ). The Δ mid1 mutants exhibited a >12-fold reduction in ascospore discharge activity and produced predominately abnormal two-celled ascospores with constricted and fragile septae. The vegetative growth rate of the mutants was ∼50% of the wild-type rate, and production of macroconidia was >10-fold lower than in the wild type. To better understand the role of calcium flux, Δ mid1 Δ cch1 double mutants were also examined, as Cch1, an L-type calcium ion channel, is associated with Mid1 in Saccharomyces cerevisiae . The phenotype of the Δ mid1 Δ cch1 double mutants was similar to but more severe than the phenotype of the Δ mid1 mutants for all categories. Potential and current-voltage measurements were taken in the vegetative hyphae of the Δ mid1 and Δ cch1 mutants and the wild type, and the measurements for all three strains were remarkably similar, indicating that neither protein contributes significantly to the overall electrical properties of the plasma membrane. Pathogenicity of the Δ mid1 and Δ mid1 Δ cch1 mutants on the host (wheat) was not affected by the mutations. Exogenous calcium supplementation partially restored the ascospore discharge and vegetative growth defects for all mutants, but abnormal ascospores were still produced. These results extend the known roles of Mid1 to ascospore development and forcible discharge. However, Neurospora crassa Δ mid1 mutants were also examined and did not exhibit defects in ascospore development or in ascospore discharge. In comparison to ion channels in other ascomycetes, Mid1 shows remarkable adaptability of roles, particularly with regard to niche-specific adaptation.

Screening of Novel Inhibitors Against Leishmania donovani Calcium ion Channel to Fight Leishmaniasis

2017 ◽  
Vol 17 (2) ◽  
Author(s):  
Mohammad Kashif ◽  
Partha P. Manna ◽  
Yusuf Akhter ◽  
Mohammed Alaidarous ◽  
Abdur Rub
Keyword(s):  
Ion Channel ◽  
Calcium Ion ◽  
Leishmania Donovani

Gibberella zeae. [Descriptions of Fungi and Bacteria].

1973 ◽  
Author(s):  
C. Booth
Keyword(s):  
Geographical Distribution ◽  
Secondary Infection ◽  
Brown Rot ◽  
Gibberella Zeae ◽  
Infested Soil ◽  
Foot Rot ◽  
Seedling Blight ◽  
Water Droplets ◽  
Ascospore Discharge ◽  
The Tropics

Abstract A description is provided for Gibberella zeae. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Wheat, maize, barley, carnations and other ornamentals; also reported infecting Lycopersicon, Pisum, Trifolium and Solanum DISEASE: Seedling blight, pre-emergence and post-emergence blight, root and foot rot, brown rot, culm decay, head or kernel blight (scab or ear scab) of wheat, maize, barley and other cereals. Leaf and flower rot of carnations and other ornamentals. Also reported infecting species of Lycopersicon, Pisum, Trifolium and Solanum. GEOGRAPHICAL DISTRIBUTION: Worldwide on maize and rice in the tropics. Wheat, oats, barley and rye in temperate regions. TRANSMISSION: By planting infected or infested seeds or by planting in infested soil. Secondary infection occurs widely by water droplets under moist conditions or by ascospore discharge.

The neuronal calcium ion channel activity of constrained analogues of MONIRO-1

2020 ◽  
Vol 28 (18) ◽  
pp. 115655
Author(s):  
Fernanda C. Cardoso ◽  
Marie-Adeline Marliac ◽  
Chloe Geoffroy ◽  
Matthieu Schmit ◽  
Anjie Bispat ◽  
...  
Keyword(s):  
Ion Channel ◽  
Calcium Ion ◽  
Channel Activity

Sporulation of Gibberella Zeae. VI. Sporulation and Maximal Mycelial Growth Occur Simultaneously

Mycologia ◽  
1980 ◽  
Vol 72 (6) ◽  
pp. 1231-1235
Author(s):  
Bor-Fuei Huang ◽  
Raymond A. Cappellini
Keyword(s):  
Mycelial Growth ◽  
Gibberella Zeae

scholarly journals Hybridization-based capture of pathogen mRNA enables paired host-pathogen transcriptional analysis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Viktoria Betin ◽  
Cristina Penaranda ◽  
Nirmalya Bandyopadhyay ◽  
Rui Yang ◽  
Angela Abitua ◽  
...  
Keyword(s):  
Transcriptional Profiling ◽  
Transcript Abundance ◽  
Abundant Species ◽  
Single Step ◽  
Sequencing Depth ◽  
Transcriptional Analysis ◽  
Rna Seq ◽  
Low Input ◽  
Temporal Gene Expression ◽  
Host Pathogen

AbstractDual transcriptional profiling of host and bacteria during infection is challenging due to the low abundance of bacterial mRNA. We report Pathogen Hybrid Capture (PatH-Cap), a method to enrich for bacterial mRNA and deplete bacterial rRNA simultaneously from dual RNA-seq libraries using transcriptome-specific probes. By addressing both the differential RNA content of the host relative to the infecting bacterium and the overwhelming abundance of uninformative structural RNAs (rRNA, tRNA) of both species in a single step, this approach enables analysis of very low-input RNA samples. By sequencing libraries before (pre-PatH-Cap) and after (post-PatH-Cap) enrichment, we achieve dual transcriptional profiling of host and bacteria, respectively, from the same sample. Importantly, enrichment preserves relative transcript abundance and increases the number of unique bacterial transcripts per gene in post-PatH-Cap libraries compared to pre-PatH-Cap libraries at the same sequencing depth, thereby decreasing the sequencing depth required to fully capture the transcriptional profile of the infecting bacteria. We demonstrate that PatH-Cap enables the study of low-input samples including single eukaryotic cells infected by 1–3 Pseudomonas aeruginosa bacteria and paired host-pathogen temporal gene expression analysis of Mycobacterium tuberculosis infecting macrophages. PatH-Cap can be applied to the study of a range of pathogens and microbial species, and more generally, to lowly-abundant species in mixed populations.

Physiological and Environmental Aspects of Ascospore Discharge in Gibberella zeae (Anamorph Fusarium graminearum)

Mycologia ◽  
2002 ◽  
Vol 94 (2) ◽  
pp. 181 ◽  
Author(s):  
Frances Trail ◽  
Haixin Xu ◽  
Rachel Loranger ◽  
David Gadoury
Keyword(s):  
Fusarium Graminearum ◽  
Gibberella Zeae ◽  
Environmental Aspects ◽  
Ascospore Discharge

Ischemic brain damage: reduction by sodium-calcium ion channel modulator RS-87476.

Radiology ◽  
1991 ◽  
Vol 179 (1) ◽  
pp. 221-227 ◽  
Author(s):  
J Kucharczyk ◽  
J Mintorovitch ◽  
M E Moseley ◽  
H S Asgari ◽  
R J Sevick ◽  
...  
Keyword(s):  
Ion Channel ◽  
Brain Damage ◽  
Calcium Ion ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Damage Reduction ◽  
Sodium Calcium ◽  
Ion Channel Modulator ◽  
Channel Modulator

scholarly journals A Comparison of Methods Used to Estimate the Maturity and Release of Ascospores of Venturia inaequalis

Plant Disease ◽  
2004 ◽  
Vol 88 (8) ◽  
pp. 869-874 ◽  
Author(s):  
David M. Gadoury ◽  
Robert C. Seem ◽  
William E. MacHardy ◽  
Wayne F. Wilcox ◽  
David A. Rosenberger ◽  
...  
Keyword(s):  
Venturia Inaequalis ◽  
Infected Leaf ◽  
Degree Days ◽  
Site Specific ◽  
Degree Day ◽  
Ascospore Discharge ◽  
Ascospore Development ◽  
Ascospore Release ◽  
The Mean ◽  
Highly Correlated

Maturation and release of ascospores of Venturia inaequalis were assessed at Geneva and Highland, NY, and at Durham, NH, by microscopic examination of crushed pseudothecia excised from infected apple leaves that were collected weekly from orchards (squash mounts) in 14 siteyear combinations. Airborne ascospore dose was monitored at each location in each year of the study by volumetric spore traps. Additional laboratory assessments were made at Geneva to quantify release from infected leaf segments upon wetting (discharge tests). Finally, ascospore maturity was estimated for each location using a degree-day model developed in an earlier study. Ascospore maturation and release determined by squash mounts and discharge tests lagged significantly behind cumulative ascospore release as measured by volumetric spore traps in the field. The mean date of 98% ascospore discharge as determined by squash mounts or discharge tests occurred from 23 to 28 days after the mean date on which 98% cumulative ascospore release had been detected by volumetric traps. In contrast, cumulative ascospore maturity estimated by the degree-day model was highly correlated (r2 = 0.82) with observed cumulative ascospore release as monitored by the volumetric traps. Although large differences between predicted maturity and observed discharge were common during the exponential phase of ascospore development, the date of 98% cumulative ascospore maturity predicted by the model was generally within 1 to 9 calendar days of the date of 98% cumulative ascospore recovery in the volumetric traps. Cumulative ascospore discharge as monitored by the volumetric traps always exceeded 98% at 600 degree days (base = 0°C) after green tip. Estimating the relative quantity of primary inoculum indirectly by means of a degree-day model was more closely aligned with observed ascospore release, as measured by volumetric traps, than actual assessments of ascospore maturity and discharge obtained through squash mounts and discharge tests. The degree-day model, therefore, may be a more accurate predictor of ascospore depletion than squash mounts or discharged tests, and has the added advantage that it can be widely applied to generate site-specific estimates of ascospore maturity for any location where daily temperature data are available.

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