scholarly journals Complete Genome Sequence of a Reference Stock of Simian Immunodeficiency Virus RNA (SIVmac251/32H/L28) Determined by Deep Sequencing

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Adrian Jenkins ◽  
Claire Ham ◽  
Neil Almond ◽  
Neil Berry
Keyword(s):  
Simian Immunodeficiency Virus ◽  
Deep Sequencing ◽  
Complete Genome ◽  
Long Terminal Repeats ◽  
Rna Quantification ◽  
Virus Rna ◽  
Immunodeficiency Virus ◽  
Minority Species ◽  
Reference Preparation ◽  
Nucleic Acid Assays

A reference preparation for simian immunodeficiency virus (SIV) RNA nucleic acid assays was characterized by complete genome deep sequencing. The entire coding sequence and flanking long terminal repeats, including minority species, were determined. This information will inform SIV research investigations and aid evaluation and development of amplification assays for SIV RNA quantification.

scholarly journals Complete Genome Sequence of the WHO International Standard for HIV-2 RNA Determined by Deep Sequencing

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Claire Ham ◽  
Clare Morris ◽  
Neil Berry
Keyword(s):  
Deep Sequencing ◽  
Complete Genome ◽  
World Health ◽  
Design Development ◽  
Long Terminal Repeats ◽  
International Standard ◽  
Minority Species ◽  
Health Organization ◽  
Subtype A ◽  
Nucleic Acid Assays

The World Health Organization (WHO) International Standard for HIV-2 RNA nucleic acid assays was characterized by complete genome deep sequencing. The entire coding sequence and flanking long terminal repeats (LTRs), including minority species, were assigned subtype A. This information will aid design, development, and evaluation of HIV-2 RNA amplification assays.

scholarly journals Comparative Evaluation of Two Branched-DNA Human Immunodeficiency Virus Type 1 RNA Quantification Assays with Lower Detection Limits of 50 and 500 Copies per Milliliter

2000 ◽  
Vol 38 (2) ◽  
pp. 914-917 ◽  
Author(s):  
Christoph Manegold ◽  
Catharina Krempe ◽  
Helmut Jablonowski ◽  
Lasse Kajala ◽  
Manfred Dietrich ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Comparative Evaluation ◽  
Plasma Samples ◽  
Rna Quantification ◽  
Branched Dna ◽  
Virus Rna ◽  
Immunodeficiency Virus ◽  
Lower Detection ◽  
Version 2.0

We have comparatively evaluated Quantiplex version 3.0 and version 2.0 on 133 plasma samples and a repetitive dilution series. Version 3.0 yielded higher human immunodeficiency virus RNA values, and the ratio of version 3.0 results to version 2.0 results decreased from 3.47 below 1,000 copies/ml to 1.97 above 50,000 copies/ml [linear regression, log (version 3.0) = 0.915 + 0.871 × log (version 2.0);r 2 = 0.952].

Real-Time TaqMan PCR as a Specific and More Sensitive Alternative to the Branched-Chain DNA Assay for Quantitation of Simian Immunodeficiency Virus RNA

2001 ◽  
Vol 17 (3) ◽  
pp. 243-251 ◽  
Author(s):  
Christian M. Leutenegger ◽  
Joanne Higgins ◽  
Timothy B. Matthews ◽  
Alice F. Tarantal ◽  
Paul A. Luciw ◽  
...  
Keyword(s):  
Real Time ◽  
Simian Immunodeficiency Virus ◽  
Dna Assay ◽  
Virus Rna ◽  
Immunodeficiency Virus ◽  
Taqman Pcr ◽  
Branched Chain

Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction

1992 ◽  
Vol 37 (2) ◽  
pp. 109-117 ◽  
Author(s):  
David J. Blackbourn ◽  
Linda F. Chuang ◽  
Suganto Sutjipto ◽  
Keith F. Killam ◽  
Paula M. McCready ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Simian Immunodeficiency Virus ◽  
Rhesus Macaques ◽  
Chain Reaction ◽  
Virus Rna ◽  
Immunodeficiency Virus ◽  
Polymerase Chain
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