scholarly journals Characterization of the selective inhibition of growth of virulent Legionella pneumophila by supplemented Mueller-Hinton medium.

1989 ◽  
Vol 57 (6) ◽  
pp. 1862-1864 ◽  
Author(s):  
C E Catrenich ◽  
W Johnson
Keyword(s):  
Legionella Pneumophila ◽  
Selective Inhibition ◽  
Inhibition Of Growth ◽  
Mueller Hinton

Characterization of class II fumarase from Schistosoma mansoni provides the molecular basis for selective inhibition

2021 ◽  
Vol 175 ◽  
pp. 406-421
Author(s):  
Iara Aimê Cardoso ◽  
Aline Kusumota Luiz de Souza ◽  
Adam Muslem George Burgess ◽  
Iain Wyllie Chalmers ◽  
Karl Francis Hoffmann ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Molecular Basis ◽  
Selective Inhibition ◽  
Class Ii

scholarly journals Molecular characterization of a virulence-associated epitope on the lipopolysaccharide ofLegionella pneumophilaserogroup 1

1995 ◽  
Vol 115 (1) ◽  
pp. 71-78 ◽  
Author(s):  
J. H. Helbig ◽  
P. C. Lück ◽  
Y. A. Knirel ◽  
W. Witzleb ◽  
U. Zähringer
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Legionella Pneumophila ◽  
Reactivity Pattern ◽  
Virulence Marker ◽  
Nonulosonic Acid ◽  
Acetyl Group

SummaryFor identification of lipopolysaccharide (LPS)-associated epitopes ofLegionella pneumophilaserogroup 1, LPS of strain Philadelphia 1 was investigated using monoclonal antibodies (MAbs). The O-specific chain of LPS is a homopolymer of 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-D-glycero-L-galacto-nonulosonic acid. At least four immunoaccessible epitopes were recognized by different MAbs on the intact LPS. AfterO-deacetylation of LPS, the reactivity of one of the MAbs (MAb 3/1) was lost, indicating thus that the corresponding epitope is associated with the 8-O-acetyl group. Since the reactivity pattern of the MAb 3/1 is identical with those of the MAb 2 which was considered as a virulence marker for serogroup 1, this epitope may be involved in mediating virulence inL. pneumophila. Four MAbs specific to strains of serogroup 1 other than the monoclonal subtype Philadelphia recognized epitopes on theO-deacetylated LPS of strain Philadelphia 1 and, therefore, the virulence-associated epitope blocks recognition of the immunodeterminants that are accessible on the intact LPS of the strains lacking this epitope.

scholarly journals Characterization of Histone Deacetylase 8 (HDAC8) Selective Inhibition Reveals Specific Active Site Structural and Functional Determinants

2018 ◽  
Vol 61 (22) ◽  
pp. 10000-10016 ◽  
Author(s):  
Martin Marek ◽  
Tajith B. Shaik ◽  
Tino Heimburg ◽  
Alokta Chakrabarti ◽  
Julien Lancelot ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Active Site ◽  
Selective Inhibition ◽  
Histone Deacetylase 8

scholarly journals Characterization of a Sinorhizobium melilotiATP-Binding Cassette Histidine Transporter Also Involved in Betaine and Proline Uptake

2000 ◽  
Vol 182 (13) ◽  
pp. 3717-3725 ◽  
Author(s):  
Eric Boncompagni ◽  
Laurence Dupont ◽  
Tam Mignot ◽  
Magne Østeräs ◽  
Annie Lambert ◽  
...  
Keyword(s):  
Glycine Betaine ◽  
Sinorhizobium Meliloti ◽  
Binding Protein ◽  
Compatible Solutes ◽  
Site Directed Mutagenesis ◽  
Uptake System ◽  
Periplasmic Binding Protein ◽  
Gene Encoding ◽  
Inhibition Of Growth

ABSTRACT The symbiotic soil bacterium Sinorhizobium melilotiuses the compatible solutes glycine betaine and proline betaine for both protection against osmotic stress and, at low osmolarities, as an energy source. A PCR strategy based on conserved domains in components of the glycine betaine uptake systems from Escherichia coli(ProU) and Bacillus subtilis (OpuA and OpuC) allowed us to identify a highly homologous ATP-binding cassette (ABC) binding protein-dependent transporter in S. meliloti. This system was encoded by three genes (hutXWV) of an operon which also contained a fourth gene (hutH2) encoding a putative histidase, which is an enzyme involved in the first step of histidine catabolism. Site-directed mutagenesis of the gene encoding the periplasmic binding protein (hutX) and of the gene encoding the cytoplasmic ATPase (hutV) was done to study the substrate specificity of this transporter and its contribution in betaine uptake. These mutants showed a 50% reduction in high-affinity uptake of histidine, proline, and proline betaine and about a 30% reduction in low-affinity glycine betaine transport. When histidine was used as a nitrogen source, a 30% inhibition of growth was observed inhut mutants (hutX and hutH2). Expression analysis of the hut operon determined using ahutX-lacZ fusion revealed induction by histidine, but not by salt stress, suggesting this uptake system has a catabolic role rather than being involved in osmoprotection. To our knowledge, Hut is the first characterized histidine ABC transporter also involved in proline and betaine uptake.

Isolation and characterization of a seventh serogroup of Legionella pneumophila.

1983 ◽  
Vol 17 (2) ◽  
pp. 346-348 ◽  
Author(s):  
W F Bibb ◽  
P M Arnow ◽  
D L Dellinger ◽  
S R Perryman
Keyword(s):  
Legionella Pneumophila ◽  
Isolation And Characterization

In Vitro Cellular Muscle Calcium Metabolism. Characterization of Effects of 1,25-Dihydroxy-Vitamin D3 and 25-Hydroxy-Vitamin D3

1985 ◽  
Vol 40 (1-2) ◽  
pp. 102-108 ◽  
Author(s):  
Ana R. de Boland ◽  
Ricardo Boland
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Plasma Membranes ◽  
Selective Inhibition ◽  
Ruthenium Red ◽  
Ca Uptake ◽  
25 Hydroxy Vitamin D ◽  
Muscle Calcium

Cultures of vitamin D-deficient chick soleus muscle and 12 day-old chick embryo myoblasts were used to characterize the effects of 1,25-dihydroxy-vitamin D3 and 25-hydroxy-vitamin D3 on muscle cell Ca metabolism. Physiological amounts of both sterols increased the rate and extent of 45Ca uptake by cultures. However. 1.25(OH)2D3 was significantly more effective than 25 OHD3. The greater potency of 1,25(OH)2D3 to increase Ca uptake could be shown after various treatment intervals of cultures and using a wide concentration range of both derivatives. Information about Ca pools affected by vitamin D3 metabolites was obtained through kinetic analysis of Ca efflux in cultured myoblasts. Cytoplasmic and mitochondria Ca pools were identified on the basis of their half-times of desaturation and by selective inhibition of plasma membrane and mitochondrial Ca transport with LaCl3 and Ruthenium Red, respectively. The data suggests that 1,25(OH)2D3 acts on muscle cellular Ca by increasing Ca efflux and influx through mitochondrial and plasma membranes whereas the predominant effect of 25 OHD3 is to increase Ca influx into mitochondria.

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