In Vitro Cellular Muscle Calcium Metabolism. Characterization of Effects of 1,25-Dihydroxy-Vitamin D3 and 25-Hydroxy-Vitamin D3

1985 ◽  
Vol 40 (1-2) ◽  
pp. 102-108 ◽  
Author(s):  
Ana R. de Boland ◽  
Ricardo Boland
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Plasma Membranes ◽  
Selective Inhibition ◽  
Ruthenium Red ◽  
Ca Uptake ◽  
25 Hydroxy Vitamin D ◽  
Muscle Calcium

Cultures of vitamin D-deficient chick soleus muscle and 12 day-old chick embryo myoblasts were used to characterize the effects of 1,25-dihydroxy-vitamin D3 and 25-hydroxy-vitamin D3 on muscle cell Ca metabolism. Physiological amounts of both sterols increased the rate and extent of 45Ca uptake by cultures. However. 1.25(OH)2D3 was significantly more effective than 25 OHD3. The greater potency of 1,25(OH)2D3 to increase Ca uptake could be shown after various treatment intervals of cultures and using a wide concentration range of both derivatives. Information about Ca pools affected by vitamin D3 metabolites was obtained through kinetic analysis of Ca efflux in cultured myoblasts. Cytoplasmic and mitochondria Ca pools were identified on the basis of their half-times of desaturation and by selective inhibition of plasma membrane and mitochondrial Ca transport with LaCl3 and Ruthenium Red, respectively. The data suggests that 1,25(OH)2D3 acts on muscle cellular Ca by increasing Ca efflux and influx through mitochondrial and plasma membranes whereas the predominant effect of 25 OHD3 is to increase Ca influx into mitochondria.

scholarly journals Suboptimal Serum 25-Hydroxy-Vitamin D Is Associated with a History of Recent Disease Exacerbation in Pediatric Patients with Bronchial Asthma or Asthma-Suggestive Recurrent Wheezing

2020 ◽  
Vol 17 (18) ◽  
pp. 6545
Author(s):  
Teodora-Irina Adam-Bonci ◽  
Paraschiva Cherecheș-Panța ◽  
Eduard-Alexandru Bonci ◽  
Sorin Claudiu Man ◽  
Ancuța Cutaș-Benedec ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Pediatric Asthma ◽  
Asthma Severity ◽  
Cross Sectional Study ◽  
Disease Exacerbation ◽  
Recurrent Wheezing ◽  
Cross Sectional ◽  
25 Hydroxy Vitamin D ◽  
History Of

Even though vitamin D is widely acknowledged as having a potential immunomodulatory role in asthma, its exact beneficial mechanisms are yet to be clarified. An optimal serum 25-hydroxy-vitamin D (25-OH-VitD) level in pediatric asthma patients might not rely solely on the effect of dose-dependent vitamin D3 intake, but might also be influenced by factors related to insufficient asthma control. We aimed to survey the prevalence of serum 25-OH-VitD deficiency and analyze whether suboptimal levels were associated with asthma severity factors. The current cross-sectional study enrolled 131 pediatric asthma or asthma-suggestive recurrent wheezing patients, for whom serum 25-OH-VitD, IgE, and eosinophil count were assessed. The prevalence of suboptimal serum 25-OH-VitD was 58.8%. A suboptimal vitamin D status was associated with asthma exacerbation in the previous month (p = 0.02). Even under seasonal oral vitamin D3 supplementation, patients with a positive history of asthma attack in the previous four weeks presented significantly lower serum 25-OH-VitD concentrations, compared to their peers with no disease exacerbation. In conclusion, sequential measurements of serum 25-OH-VitD might prove useful for future studies evaluating the dynamic changes in vitamin D3 status in regard to asthma, especially in symptomatic patients.

Stimulation of Myoblast Membrane Protein Synthesis by 25-Hydroxy-Vitamin D 3

1989 ◽  
Vol 44 (9-10) ◽  
pp. 807-812
Author(s):  
Teresita Bellido ◽  
Ricardo Boland
Keyword(s):  
Vitamin D ◽  
Protein Synthesis ◽  
Plasma Membranes ◽  
De Novo ◽  
Phosphate Uptake ◽  
Leucine Incorporation ◽  
Similar Treatment ◽  
25 Hydroxy Vitamin D ◽  
Alpha Bungarotoxin ◽  
Stimulation Of

Abstract The effects of 25-hydroxy-vitamin D3 (25 OHD3) on myoblast protein synthesis were studied in connection with its role on muscle cell phosphate metabolism . The sterol markedly increased leucine incorporation into total cell proteins in cultured chick embryo myoblasts. This enhance­ment was greater than that produced by 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3) and occurred prior to a significant stimulation of cell phosphate accumulation. Maximum effects of 25 OHD3 (8 h) on myoblast phosphate uptake were suppressed by cycloheximide indicating that they are mediated by de novo protein synthesis. At a similar treatment period, labelling of myoblasts with [3H]leucine (control) and [14C]leucine (+ 25 OHD3) followed by co-electrophoresis of total protein extracts on SDS-PAGE and isoelectrofocusing gels revealed that the sterol selectively affects the synthesis of proteins of 20 kDa and 50 kDa. These macromolecules were recovered in the microsomal fraction after differential centrifugation of homogenates. Further fractionation of myoblast microsomes on sucrose density gradients show ed co-localization of the 50 kDa and 20 kDa proteins with microsomal subfractions which preferentially bind [3H -alpha]bungarotoxin, suggesting that the proteins induced by 25 OHD3 are associated to plasma membranes and may play a role in the effects of the sterol on cell phosphate uptake.

scholarly journals Development and Characterization of an Orodispersible Film for Vitamin D3 Supplementation

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5851
Author(s):  
Irma Elisa Cupone ◽  
Eleonora Dellera ◽  
Fabio Marra ◽  
Andrea Maria Giori
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Dosage Form ◽  
Daily Intake ◽  
Stability Study ◽  
Food Supplements ◽  
Oral Dosage ◽  
Residual Water ◽  
Tract Infections

Vitamin D plays a crucial and very well-known role in regulation of calcium homeostasis and bone metabolism and mineralization. However, a huge and more recent body of evidence supports the positive influence of vitamin D on the regulation of immune response, ranging from protection against respiratory tract infections to prevention and management of asthma. Nevertheless, vitamin D deficiency is a very common condition and there is an increasing need for suitable products for proper supplementation, allowing good compliance also in specific populations. Orally disintegrating tablets (ODT) were first developed to overcome the difficulty experienced by pediatric and geriatric patients of swallowing traditional oral dosage forms and, recently, orodispersible films (ODF) are gaining popularity as novel dosage form for assuming active pharmaceutical ingredients, vitamins, and ingredients for food supplements. This study describes a 2000 IU Vitamin D3 ODF for daily intake, consisting of hydrophilic polymers and suitable excipients, manufactured by film-casting process. Elongation-at-break (E%), Young’s modulus (Y), and tensile strength (TS) were investigated using a dynamometer. Chemical stability was evaluated assaying the vitamin D3 in the films stored at different environmental conditions. In addition, in vitro disintegration and dissolution studies were performed. Correlation existed between the mechanical properties of the film and the residual water, acting as plasticizer. The stability study showed that vitamin D3 assay was ≥90% also after 3 months at 40 °C. The film disintegrated in less than 1 min and the vitamin D3 released was ≥75% after 15 min. An ODF with suitable properties can be manufactured and used as innovative dosage form for vitamin D3 food supplements.

Renal effect of vitamin D metabolites: evidence for the essential role of the 25(OH) group

1983 ◽  
Vol 244 (6) ◽  
pp. F674-F678 ◽  
Author(s):  
M. M. Friedlaender ◽  
Z. Kornberg ◽  
H. Wald ◽  
M. M. Popovtzer
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Essential Role ◽  
Fractional Excretion ◽  
Vitamin D Metabolites ◽  
Group 2 ◽  
Fractional Excretion Of Phosphate ◽  
Group 1

The effects of 1 alpha (OH)vitamin D3 [1 alpha (OH)D3] and 24,25(OH)2vitamin D3 [24,25(OH)2D3] on the phosphaturic action of parathyroid hormone (PTH) were studied in two groups of parathyroidectomized (PTX) rats. In group 1, PTX PTH-infused rats received intravenous 1 alpha (OH)D3, and in group 2, PTX PTH-infused rats received intravenous 24,25(OH)2D3. PTX PTH-infused rats served as controls. The effects of both vitamin D metabolites on renal PTH-activated adenylate cyclase (AC) were studied in vitro. In group 1, PTH increased fractional excretion of phosphate (CP/CIn) from 0.045 +/- 0.012 (+/- SE) to 0.263 +/- 0.011 (P less than 0.005). 1 alpha (OH)D3 failed to influence this response. In group 2, PTH increased CP/CIn from 0.055 +/- 0.008 to 0.289 +/- 0.027 (P less than 0.005). 24,25(OH)2D3 reduced the PTH-induced rise in CP/CIn from 0.289 +/- 0.027 to 0.192 +/- 0.021 (P less than 0.01) and decreased the urinary excretion of adenosine 3',5'-cyclic monophosphate. In vitro, 24,25(OH)2D3 blunted the PTH-activated AC, whereas 1 alpha (OH)D3 had no effect. These results show that 24,25(OH)D3, similar to two other 25(OH) metabolites of vitamin D-25(OH)vitamin D3 and 1,25(OH)2vitamin D3-suppresses the phosphaturic action of PTH, whereas 1 alpha(OH)D3, which is devoid of a 25(OH) group, lacks this effect. This suggests that a 25(OH) group is a prerequisite for the antiphosphaturic effect of vitamin D, whereas the 1 alpha (OH) group is not essential for this action.

scholarly journals Vitamin D enhances responses to interferon-β in MS

2019 ◽  
Vol 6 (6) ◽  
pp. e622 ◽  
Author(s):  
Xuan Feng ◽  
Zhe Wang ◽  
Quentin Howlett-Prieto ◽  
Nathan Einhorn ◽  
Suad Causevic ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Immune Regulation ◽  
Mononuclear Cells ◽  
Serum Proteins ◽  
Multiplex Assay ◽  
Interferon Β ◽  
Western Blots ◽  
Th17 Cytokines

ObjectiveTo determine the effect of vitamin D3 on interferon-β (IFN-β) response and immune regulation in MS mononuclear cells (MNCs).MethodsMNCs from 126 subjects, including therapy-naive patients with different forms of MS, plus patients with MS receiving IFN-β or glatiramer treatment, plus healthy controls were incubated in vitro with IFN-β-1b ± vitamin D3 (calcitriol). Activation of the IFN-β–induced transcription factor, p-Y-STAT1, and antiviral myxovirus A (MxA) protein was measured with flow cytometry and Western blots; serum proteins were measured with a customized 31-protein multiplex assay.ResultsVitamin D enhanced in vitro IFN responses, as measured by induction of p-Y-STAT1 and MxA in MNCs, T cells, and monocytes. Vitamin D augmentation of IFN responses was seen in untreated and in IFN-β-1b–treated MS. The combination of vitamin D plus IFN-β reduced Th1 and Th17 cytokines, and increased Th2 responses, reversing the effect of IFN-β alone. Exacerbations and progression in untreated patients reduced the vitamin D enhancement of IFN responses. Vitamin D had less effect on IFN response in clinically stable glatiramer-treated than in IFN-β–treated patients.ConclusionVitamin D enhances IFN-β induction of multiple proteins and also reverses the Th1/Th2 bias in MS seen with IFN-β alone. The combination of vitamin D and IFN-β has potential benefit in ameliorating MS.

scholarly journals Selective inhibition of the C5a chemotactic cofactor function of the Vitamin D binding protein by 1,25(OH)2 Vitamin D3

2006 ◽  
Vol 43 (8) ◽  
pp. 1109-1115 ◽  
Author(s):  
Anisha B. Shah ◽  
Stephen J. DiMartino ◽  
Glenda Trujillo ◽  
Richard R. Kew
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Binding Protein ◽  
Selective Inhibition ◽  
Vitamin D Binding Protein

scholarly journals The Relationship between Obesity and Serum 1,25-Dihydroxy Vitamin D Concentrations in Healthy Adults

2004 ◽  
Vol 89 (3) ◽  
pp. 1196-1199 ◽  
Author(s):  
Shamik J. Parikh ◽  
Marni Edelman ◽  
Gabriel I. Uwaifo ◽  
Renee J. Freedman ◽  
Mariama Semega-Janneh ◽  
...  
Keyword(s):  
Vitamin D ◽  
African American ◽  
Body Fat ◽  
Fat Mass ◽  
Healthy Adults ◽  
Body Fat Mass ◽  
Intact Pth ◽  
Hormonal Mechanism ◽  
25 Hydroxy Vitamin D

Abstract Several previous reports of small cohorts have found significantly higher serum 1,25-dihydroxy vitamin D (1,25-vit D) in obese compared with nonobese whites. Based on these reports and on recent in vitro studies of adipocytes which suggest that administration of 1,25-vit D can stimulate lipogenesis and inhibit lipolysis, some investigators have proposed that high 1,25-vit D may play a role in promoting or maintaining adipocyte triglyceride stores in obese adults. To test the hypothesis that obesity is commonly associated with increased 1,25-vit D, we examined the relationships between calciotropic hormones and body adiposity in a large cohort of healthy adults. Serum intact PTH, 25-hydroxy vitamin D, and 1,25-vit D were measured in the postabsorptive state in 302 healthy adults who were Caucasian (n = 190; 71% female), African-American (n = 84; 89% female), and of other race/ethnicity (n = 28; 61% female). Results from the 154 obese subjects [body mass index (BMI) 37.3 ± 5.8 kg/m2; range, 30.1–58.2 kg/m2] were compared with those from 148 nonobese (BMI 25.6 ± 2.9 kg/m2; range, 18.0–29.9 kg/m2) age-, race-, and sex-matched participants. Body composition was measured by dual energy x-ray absorptiometry. Serum intact PTH was positively correlated with both BMI (r = 0.42; P < 0.0001) and body fat mass (r = 0.37; P < 0.0001). Serum 25-hydroxy vitamin D was negatively correlated with BMI (r = −0.4; P < 0.0001) and body fat mass (r = −0.41; P < 0.0001). Serum 1,25-vit D was also negatively correlated with BMI (r = −0.26; P < 0.0001) and body fat mass (r = −0.25; P = 0.0001). Serum 1,25-vit D was significantly lower in obese than nonobese subjects (105.7 ± 41.1 vs. 124.8 ± 36.7 pmol/liter; P < 0.0001) in both Caucasian and African-American adults. We conclude that, because 1,25-vit D concentrations fall with increasing adiposity, it appears unlikely that elevation in 1,25-vit D is an important hormonal mechanism causing or maintaining obesity in adults.

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