scholarly journals Immunogenicity and antigenicity in rabbits of a repeated sequence of Plasmodium falciparum antigen Pf155/RESA fused to two immunoglobulin G-binding domains of staphylococcal protein A.

1990 ◽  
Vol 58 (4) ◽  
pp. 854-859 ◽  
Author(s):  
A Sjölander ◽  
S Ståhl ◽  
P A Nygren ◽  
L Aslund ◽  
N Ahlborg ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunoglobulin G ◽  
Protein A ◽  
Repeated Sequence ◽  
Staphylococcal Protein A ◽  
Staphylococcal Protein ◽  
Binding Domains

NMR Study of the Interaction between the B Domain of Staphylococcal Protein A and the Fc Portion of Immunoglobulin G

Biochemistry ◽  
1998 ◽  
Vol 37 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Hiroaki Gouda ◽  
Miki Shiraishi ◽  
Hideo Takahashi ◽  
Koichi Kato ◽  
Hidetaka Torigoe ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Staphylococcal Protein ◽  
Nmr Study

scholarly journals 13 C NMR study of the mode of interaction in solution of the B fragment of staphylococcal protein A and the Fc fragments of mouse immunoglobulin G

FEBS Letters ◽  
1993 ◽  
Vol 328 (1-2) ◽  
pp. 49-54 ◽  
Author(s):  
Koichi Kato ◽  
Hiroaki Gouda ◽  
Wakana Takaha ◽  
Atsuko Yoshino ◽  
Chigusa Matsunaga ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Staphylococcal Protein ◽  
Nmr Study ◽  
Mouse Immunoglobulin ◽  
C Nmr

scholarly journals Use of fluorescence flow cytometry to study the binding of various ligands to platelets.

1985 ◽  
Vol 33 (11) ◽  
pp. 1176-1179 ◽  
Author(s):  
R A Dunstan
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Intensity ◽  
Immunoglobulin G ◽  
Protein A ◽  
Fluorescein Isothiocyanate ◽  
Staphylococcal Protein A ◽  
Careful Choice ◽  
Fluorescence Studies ◽  
Staphylococcal Protein

Fluorescence flow cytometry can be used to analyze the binding of different ligands to platelets. However careful choice of volume gates is essential in selecting the population of platelets for analysis. The use of fluorescein isothiocyanate conjugated to staphylococcal protein A or F(ab')2 fragments of immunoglobulin G anti-immunoglobulin offers no advantage in sensitivity or specificity in fluorescence studies of platelets and prefixation of washed platelets with paraformaldehyde has no effect on nonspecific fluorescence. The application of this technology to platelets facilitates quantitation of fluorescence intensity and may yield additional useful information.

scholarly journals Effect of immunoglobulin G on the responsiveness of human neutrophils to soluble staphylococcal protein A-induced neutrophil migration inhibition factor from T-lymphocytes

1980 ◽  
Vol 30 (3) ◽  
pp. 674-677
Author(s):  
K K Colburn ◽  
L G Wong ◽  
R F Ashman ◽  
R Wistar ◽  
R H Weisbart
Keyword(s):  
T Lymphocytes ◽  
Immunoglobulin G ◽  
Protein A ◽  
Neutrophil Migration ◽  
Migration Inhibition Factor ◽  
Human Neutrophils ◽  
Staphylococcal Protein A ◽  
Migration Inhibition ◽  
Staphylococcal Protein ◽  
Inhibition Factor

Staphylococcal protein A is a bacterial cell wall product that binds human immunoglobulin G and thereby interferes with opsonization and phagocytosis of Staphylococcus aureus by neutrophils. Phagocytic cells are also responsive to various non-immunoglobulin lymphocyte mediators. We utilized the detection of a newly recognized mediator, a neutrophil migration inhibition factor from T-lymphocytes (NIF-T), to show that aggregates of staphylococcal protein A and immunoglobulins G could inhibit the responsiveness of neutrophils to NIF-T. That such aggregates may alter the responsiveness of neutrophils to lymphocyte mediators that amplify or modulate phagocytic functions may have important pathogenetic implications in staphylococcal infection.

Construction and characterization of M13 bacteriophages displaying functional IgG-binding domains of Staphylococcal protein A

Gene ◽  
1994 ◽  
Vol 151 (1-2) ◽  
pp. 45-51 ◽  
Author(s):  
Ashima Kushwaha ◽  
Partha Sarathi Chowdhury ◽  
Kajal Arora ◽  
Smita Abrol ◽  
Vijay K. Chaudhary
Keyword(s):  
Protein A ◽  
Staphylococcal Protein A ◽  
Staphylococcal Protein ◽  
Binding Domains ◽  
Igg Binding
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