Virulence of a Legionella anisa strain associated with Pontiac fever: an evaluation using protozoan, cell culture, and guinea pig models.

1990 ◽  
Vol 58 (9) ◽  
pp. 3139-3142 ◽  
Author(s):  
B S Fields ◽  
J M Barbaree ◽  
G N Sanden ◽  
W E Morrill
Keyword(s):  
Cell Culture ◽  
Guinea Pig ◽  
Pontiac Fever ◽  
Protozoan Cell

Antigenicity of Guinea Pig Testicular Cells grown in Cell Culture as determined by the Isolated Ileum Technique

Nature ◽  
1961 ◽  
Vol 192 (4804) ◽  
pp. 770-771 ◽  
Author(s):  
SEYMOUR KATSH ◽  
RUSSELL T. JORDAN
Keyword(s):  
Cell Culture ◽  
Guinea Pig ◽  
Testicular Cells

Characterization of Lymphokine Fibronectin from Guinea Pig Lymphoid Cell Culture Supernatants

Immunobiology ◽  
1990 ◽  
Vol 180 (2-3) ◽  
pp. 109-123 ◽  
Author(s):  
Henry P. Godfrey ◽  
Loretto S. Canfield ◽  
Channabasappa V. Angadi ◽  
Luba M. Zagachin ◽  
Grace G. Kielpinsk ◽  
...  
Keyword(s):  
Cell Culture ◽  
Guinea Pig ◽  
Lymphoid Cell ◽  
Culture Supernatants ◽  
Lymphoid Cell Culture

scholarly journals A primary culture of guinea pig gallbladder epithelial cells that is responsive to secretagogues

2000 ◽  
Vol 279 (5) ◽  
pp. G866-G874 ◽  
Author(s):  
Pamela J. Gunter-Smith ◽  
Oluwakemi Abdulkadir ◽  
Latanya Hammonds-Odie ◽  
Mary Scanlon ◽  
Raquel Terrell
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Guinea Pig ◽  
Ion Transport ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Intestinal Epithelia ◽  
Intracellular Ca ◽  
A Cell ◽  
Transport Inhibitors

We have developed a cell culture of guinea pig gallbladder epithelial cells with which to study ion transport. When grown on permeable supports, the cultured epithelia developed a transepithelial resistance ( R t) of ∼500 Ω · cm2. The epithelial cell origin of the cell culture was further confirmed by immunocytochemical localization of cytokeratin. Ionomycin and forskolin increased transepithelial voltage and short-circuit current ( I sc) and decreased R t. The response to ionomycin was transient, whereas that to forskolin was sustained. Both were attenuated by replacement of Cl− and/or HCO3 −. Mucosal addition of the anion transport inhibitors DIDS or diphenylamine-2-carboxylic acid (DPC) blocked the response to ionomycin. The response to forskolin was blocked by DPC but not by DIDS. Ionomycin, but not forskolin, increased intracellular Ca2+ concentration in fura 2-loaded cells. PGE2, histamine, vasoactive intestinal polypeptide, and secretin elicited a sustained increase in I sc. Responses to ATP and CCK were transient. Thus cultured guinea pig gallbladder epithelia display the range of responses observed in the native tissue and are an appropriate model for studies of ion transport in gallbladder and intestinal epithelia.

scholarly journals THE SMOOTH MUSCLE CELL

1971 ◽  
Vol 50 (1) ◽  
pp. 172-186 ◽  
Author(s):  
Russell Ross
Keyword(s):  
Cell Culture ◽  
Smooth Muscle ◽  
Amino Acid ◽  
Connective Tissue ◽  
Basement Membrane ◽  
Guinea Pig ◽  
Elastic Fiber ◽  
Extracellular Proteins ◽  
Aortic Smooth Muscle ◽  
Synthetic Cell

Smooth muscle derived from the inner media and intima of immature guinea pig aorta were grown for up to 8 wk in cell culture. The cells maintained the morphology of smooth muscle at all phases of their growth in culture. After growing to confluency, they grew in multiple overlapping layers. By 4 wk in culture, microfibrils (110 A) appeared within the spaces between the layers of cells. Basement membrane-like material also appeared adjacent to the cells. Analysis of the microfibrils showed that they have an amino acid composition similar to that of the microfibrillar protein of the intact elastic fiber. These investigations coupled with the radioautographic observations of the ability of aortic smooth muscle to synthesize and secrete extracellular proteins demonstrate that this cell is a connective tissue synthetic cell.

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