scholarly journals Expression of Escherichia coli F-18 type 1 fimbriae in the streptomycin-treated mouse large intestine.

1991 ◽  
Vol 59 (4) ◽  
pp. 1567-1568 ◽  
Author(s):  
K A Krogfelt ◽  
B A McCormick ◽  
R L Burghoff ◽  
D C Laux ◽  
P S Cohen
1993 ◽  
Vol 14 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Beth A. McCormick ◽  
Per Klemm ◽  
Karen A. Krogfelt ◽  
Robert L. Burghoff ◽  
Lars Pallesen ◽  
...  

Author(s):  
K. A. Krogfelt ◽  
B. A. McCormick ◽  
R. L. Burgoff ◽  
D. C. Laux ◽  
P. S. Cohen

1996 ◽  
Vol 183 (3) ◽  
pp. 1037-1044 ◽  
Author(s):  
M Hedlund ◽  
M Svensson ◽  
A Nilsson ◽  
R D Duan ◽  
C Svanborg

Escherichia coli express fimbriae-associated adhesins through which they attach to mucosal cells and activate a cytokine response. The receptors for E. coli P fimbriae are the globoseries of glycosphingolipids; Gal alpha 1-->4Gal beta-containing oligosaccharides bound to ceramide in the outer leaflet of the lipid bilayer. The receptors for type 1 fimbriae are mannosylated glycoproteins rather than glycolipids. This study tested the hypothesis that P-fimbriated E. coli elicit a cytokine response through the release of ceramide in the receptor-bearing cell. We used the A498 human kidney cell line, which expressed functional receptors for P and type 1 fimbriae and secreted higher levels of interleukin (IL)-6 when exposed to the fimbriated strains than to isogenic nonfimbriated controls. P-fimbriated E. coli caused the release of ceramide and increased the phosphorylation of ceramide to ceramide 1-phosphate. The IL-6 response to P-fimbriated E. coli was reduced by inhibitors of serine/threonine kinases but not by other protein kinase inhibitors. In contrast, ceramide levels were not influenced by type 1-fimbriated E. coli, and the IL-6 response was insensitive to the serine/threonine kinase inhibitors. These results demonstrate that the ceramide-signaling pathway is activated by P-fimbriated E. coli, and that the receptor specificity of the P fimbriae influences this process. We propose that this activation pathway contributes to the cytokine induction by P-fimbriated E. coli in epithelial cells.


Fimbriae ◽  
2020 ◽  
pp. 9-26
Author(s):  
Per Klemm ◽  
Karen Angeliki Krogfelt

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