T-Cell Epitopes in Variable Segments ofChlamydia trachomatis Major Outer Membrane Protein Elicit Serovar-Specific Immune Responses in Infected Humans

ABSTRACT We previously identified 18 stimulatory Chlamydia trachomatis major outer membrane protein (MOMP) peptides containing at least 23 epitopes presented with various HLA class II allotypes. Only one peptide contained an epitope localized in a variable segment (VS2). Continued studies reported here identified a total of five VS peptides containing T-cell epitopes that are distributed among MOMPs VS1, VS2, and VS4. Only MOMP-primed T-cell cultures from subjects infected with serovar E responded to the serovar E VS peptides, while the response of such cultures to constant-segment peptides was independent of the infecting serovar. Furthermore, MOMP-primed T cells proliferated in response only to the VS peptides encoded in serovar E but not to the corresponding peptides derived from serovar F, I, or J, confirming that these responses were serovar specific.

Download Full-text

The effect of Chlamydophila pneumoniae Major Outer Membrane Protein (MOMP) on macrophage and T cell-mediated immune responses

Immunobiology ◽

10.1016/j.imbio.2010.06.004 ◽

2011 ◽

Vol 216 (1-2) ◽

pp. 152-163 ◽

Cited By ~ 9

Author(s):

Alexandra Bermudez-Fajardo ◽

Anne-Katrien Stark ◽

Rehab El-Kadri ◽

Manuel L. Penichet ◽

Katharina Hölzle ◽

...

Keyword(s):

T Cell ◽

Membrane Protein ◽

Immune Responses ◽

Outer Membrane ◽

Outer Membrane Protein ◽

Chlamydophila Pneumoniae ◽

Major Outer Membrane Protein

Download Full-text

Mapping of the immunodominant B- and T-cell epitopes of the outer membrane protein P6 of H. influenzae type b using overlapping synthetic peptides

Peptides ◽

10.1007/978-94-011-0683-2_241 ◽

1994 ◽

pp. 730-731

Author(s):

P. Chong ◽

O. James ◽

Y.-P. Yang ◽

C. Sia ◽

B. Tripet ◽

...

Keyword(s):

T Cell ◽

Membrane Protein ◽

Outer Membrane ◽

Outer Membrane Protein ◽

Synthetic Peptides ◽

T Cell Epitopes ◽

Type B

Download Full-text

Priming with Chlamydia trachomatis Major Outer Membrane Protein (MOMP) DNA followed by MOMP ISCOM Boosting Enhances Protection and Is Associated with Increased Immunoglobulin A and Th1 Cellular Immune Responses

Infection and Immunity ◽

10.1128/iai.68.6.3074-3078.2000 ◽

2000 ◽

Vol 68 (6) ◽

pp. 3074-3078 ◽

Cited By ~ 66

Author(s):

Zhang Dong-Ji ◽

Xi Yang ◽

Caixia Shen ◽

Hong Lu ◽

Andrew Murdin ◽

...

Keyword(s):

Chlamydia Trachomatis ◽

Membrane Protein ◽

Immune Responses ◽

Outer Membrane ◽

Outer Membrane Protein ◽

Immunoglobulin A ◽

Major Outer Membrane Protein ◽

Delayed Type Hypersensitivity ◽

Cellular Immune Responses ◽

Cellular Immune

ABSTRACT We previously reported that DNA vaccination was able to elicit cellular immune responses and partial protection againstChlamydia trachomatis infection. However, DNA immunization alone did not generate immune responses or protection as great as that induced by using live organisms. In this study, we evaluated the immunologic effects of a combinational vaccination approach usingC. trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP) DNA priming followed by boosting with immune-stimulating complexes (ISCOM) of MOMP protein (MOMP ISCOM) for protection of BALB/c mice against MoPn lung infection. Substantially better protection to challenge infection was observed in mice given combinational vaccination compared with mice given MOMP ISCOM immunization alone, and the protection approximated that induced by live organisms. Enhanced protection was correlated with stronger delayed-type hypersensitivity, higher levels of gamma interferon production, and increased immunoglobulin A antibody responses in lung homogenates. The results indicate that DNA priming followed by ISCOM protein boosting may be useful in designing a fully protective chlamydial vaccine.

Download Full-text