scholarly journals Identification of Escherichia coli YgaF as an l-2-Hydroxyglutarate Oxidase

2008 ◽  
Vol 190 (11) ◽  
pp. 3793-3798 ◽  
Author(s):  
Efthalia Kalliri ◽  
Scott B. Mulrooney ◽  
Robert P. Hausinger
Keyword(s):  
Escherichia Coli ◽  
Unknown Function ◽  
Oxidase Activity ◽  
Flavin Adenine Dinucleotide ◽  
Reduction Potential ◽  
High Reduction ◽  
Very High

ABSTRACT YgaF, a protein of previously unknown function in Escherichia coli, was shown to possess noncovalently bound flavin adenine dinucleotide and to exhibit l-2-hydroxyglutarate oxidase activity. The inability of anaerobic, reduced enzyme to reverse the reaction by reducing the product α-ketoglutaric acid is explained by the very high reduction potential (+19 mV) of the bound cofactor. The likely role of this enzyme in the cell is to recover α-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate. On the basis of the identified function, we propose that this gene be renamed lhgO.

scholarly journals Characterization of a Tricationic Trigonal Bipyramidal Iron(IV) Cyanide Complex, with a Very High Reduction Potential, and Its Iron(II) and Iron(III) Congeners

2011 ◽  
Vol 50 (7) ◽  
pp. 2885-2896 ◽  
Author(s):  
Jason England ◽  
Erik R. Farquhar ◽  
Yisong Guo ◽  
Matthew A. Cranswick ◽  
Kallol Ray ◽  
...  
Keyword(s):  
Reduction Potential ◽  
Cyanide Complex ◽  
Trigonal Bipyramidal ◽  
High Reduction ◽  
Very High

scholarly journals Role of D-aminoacyl-tRNA deacylase beyond chiral proofreading as a cellular defense against glycine mischarging by AlaRS

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Komal Ishwar Pawar ◽  
Katta Suma ◽  
Ayshwarya Seenivasan ◽  
Santosh Kumar Kuncha ◽  
Satya Brata Routh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Active Site ◽  
Unknown Function ◽  
Physiological Importance ◽  
Cellular Defense

Strict L-chiral rejection through Gly-cisPro motif during chiral proofreading underlies the inability of D-aminoacyl-tRNA deacylase (DTD) to discriminate between D-amino acids and achiral glycine. The consequent Gly-tRNAGly ‘misediting paradox’ is resolved by EF-Tu in the cell. Here, we show that DTD’s active site architecture can efficiently edit mischarged Gly-tRNAAla species four orders of magnitude more efficiently than even AlaRS, the only ubiquitous cellular checkpoint known for clearing the error. Also, DTD knockout in AlaRS editing-defective background causes pronounced toxicity in Escherichia coli even at low-glycine levels which is alleviated by alanine supplementation. We further demonstrate that DTD positively selects the universally invariant tRNAAla-specific G3•U70. Moreover, DTD’s activity on non-cognate Gly-tRNAAla is conserved across all bacteria and eukaryotes, suggesting DTD’s key cellular role as a glycine deacylator. Our study thus reveals a hitherto unknown function of DTD in cracking the universal mechanistic dilemma encountered by AlaRS, and its physiological importance.

The role of backbone stability near Ala44 in the high reduction potential class of rubredoxins

2005 ◽  
Vol 62 (3) ◽  
pp. 708-714 ◽  
Author(s):  
Ming-Liang Tan ◽  
ChulHee Kang ◽  
Toshiko Ichiye
Keyword(s):  
Reduction Potential ◽  
High Reduction

THE ROLE OF GRASSLAND IN MANAGEMENT

1968 ◽  
pp. 44-49
Author(s):  
B.K. Cameron
Keyword(s):  
Soil Temperature ◽  
Annual Rainfall ◽  
Silt Loam ◽  
Average Annual Rainfall ◽  
Very High ◽  
Wilting Point

THE PROPERTY to be discussed is a mixed sheep and cropping unit, situated ei ht a miles east of Ashburton and midway between the Ra aia and the Ashburton rivers. Average annual rainfall is 27 in., evenly spread, but there is very high summer evaporation and therefore frequent droughts. On average, the soil is below wilting point for 40 to 50 days each summer. Winters are cold with the soil temperature being below 48°F for about four months each year. The soil is a Lismore stony silt loam averaging 9 in. in depth over gravel.

Role of Vasavaleha in the management of Covid19

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 259-261
Author(s):  
Aamir Khan ◽  
Rajni K. Gurmule
Keyword(s):  
Immune System ◽  
Human Body ◽  
Respiratory Diseases ◽  
Spreading Rate ◽  
Host Immune System ◽  
Shortness Of Breath ◽  
Antitussive Effect ◽  
Corona Viruses ◽  
Very High

Vasavaleha is one of the best medicine given for respiratory diseases. Corona viruses typically affect the respiratory system, causing symptoms such as coughing, fever and shortness of breath. It also affects host immune system of human body. Spreading rate of this disease is very high. Whole world is seeking for the treatment which can uproots this diseases. There in no vaccine available till date against this pandemic disease. Ayurveda mainly focuses on prevention of diseases alongwith its total cure. Rajyakshma Vyadhi is MadhyamMarga Roga as per Ayurveda. It shows many symptoms such as Kasa, Shwasa etc. By overall view of Covid 19, shows its resemblance with Rajyakshma Vyadhi described in Ayurveda. Vasavaleha is a Kalpa which is described in Rogadhikara of Rajyakshma. It shows Kasahara, Shwashara properties. It consists of Vasa, Pipalli, Madhu and Goghrita. These components shows actions like bronchodilation, antitussive effect and many more other actions. Pipalli shows important Rasayana effect. So in present review, we have tried to focus on role of Vasavaleha in the management of Covid 19. This can be used as preventive as well as adjuvant medication in treating Covid 19. There is need of further clinical research to rule of exact action of Vasavaleha against Covid 19.

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