Oxaluric Acid: a Non-Metabolizable Inducer of the Allantoin Degradative Enzymes in Saccharomyces cerevisiae

The URA3 gene from Saccharomyces cerevisiae is localized on a 1.1-kilobase (kb) DNA fragment. By using this fragment as a hybridization probe, we found that oxalurate, a gratuitous inducer of the allantoin degradative system, also serves to induce URA3 specific RNA. This response is restricted to oxalurate; other conditions which bring about high-level synthesis of the allantoin degradative enzymes did not produce the effect. Two classes of RNA (1.0 and 1.5 kb) were found to be oxalurate induced. Both classes are encoded by the URA3 gene, overlap, and probably do not significantly differ at their 5' termini. Northern blot mapping of the transcripts indicated that the 1.5-kb transcript was likely encoded by sequences extending up to 0.5 kb downstream from the 3' terminus of the 1.0-kb transcript. Analysis of the endpoints of the major 1.0-kb URA3 transcript by S1 nuclease mapping revealed the existence of two 5' termini, separated by 5 to 10 nucleotides, and seven 3' termini, separated by 5 to 20 nucleotides each, over a range of about 70 bases.

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Possible failure of NADP-glutamate dehydrogenase to participate directly in nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(75)90723-8 ◽

1975 ◽

Vol 66 (3) ◽

pp. 889-892 ◽

Cited By ~ 11

Author(s):

June Bossinger ◽

Terrance Cooper

Keyword(s):

Saccharomyces Cerevisiae ◽

Glutamate Dehydrogenase ◽

Degradative Enzymes ◽

Nitrogen Repression

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Nitrogen Repression of the Allantoin Degradative Enzymes in Saccharomyces cerevisiae

Journal of Bacteriology ◽

10.1128/jb.118.3.821-829.1974 ◽

1974 ◽

Vol 118 (3) ◽

pp. 821-829 ◽

Cited By ~ 14

Author(s):

June Bossinger ◽

Robert P. Lawther ◽

Terrance G. Cooper

Keyword(s):

Saccharomyces Cerevisiae ◽

Degradative Enzymes ◽

Nitrogen Repression

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Oxalurate induction of multiple URA3 transcripts in Saccharomyces cerevisiae.

Molecular and Cellular Biology ◽

10.1128/mcb.3.11.1889 ◽

1983 ◽

Vol 3 (11) ◽

pp. 1889-1897 ◽

Cited By ~ 6

Author(s):

R G Buckholz ◽

T G Cooper

Keyword(s):

Saccharomyces Cerevisiae ◽

Hybridization Probe ◽

S1 Nuclease ◽

Ura3 Gene ◽

Degradative Enzymes ◽

S1 Nuclease Mapping ◽

Dna Fragment ◽

High Level ◽

Nuclease Mapping ◽

Gene Overlap

The URA3 gene from Saccharomyces cerevisiae is localized on a 1.1-kilobase (kb) DNA fragment. By using this fragment as a hybridization probe, we found that oxalurate, a gratuitous inducer of the allantoin degradative system, also serves to induce URA3 specific RNA. This response is restricted to oxalurate; other conditions which bring about high-level synthesis of the allantoin degradative enzymes did not produce the effect. Two classes of RNA (1.0 and 1.5 kb) were found to be oxalurate induced. Both classes are encoded by the URA3 gene, overlap, and probably do not significantly differ at their 5' termini. Northern blot mapping of the transcripts indicated that the 1.5-kb transcript was likely encoded by sequences extending up to 0.5 kb downstream from the 3' terminus of the 1.0-kb transcript. Analysis of the endpoints of the major 1.0-kb URA3 transcript by S1 nuclease mapping revealed the existence of two 5' termini, separated by 5 to 10 nucleotides, and seven 3' termini, separated by 5 to 20 nucleotides each, over a range of about 70 bases.

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