scholarly journals Cloning and characterization of the Escherichia coli lit gene, which blocks bacteriophage T4 late gene expression.

1987 ◽  
Vol 169 (3) ◽  
pp. 1232-1238 ◽  
Author(s):  
C Kao ◽  
E Gumbs ◽  
L Snyder
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Bacteriophage T4 ◽  
Late Gene ◽  
Late Gene Expression

scholarly journals Characterization of late gene expression factors lef-9 and lef-8 from Bombyx mori nucleopolyhedrovirus

2002 ◽  
Vol 83 (8) ◽  
pp. 2015-2023 ◽  
Author(s):  
Asha Acharya ◽  
Karumathil P. Gopinathan
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Late Gene ◽  
Late Gene Expression ◽  
Reading Frame ◽  
Early Transcription ◽  
A Site ◽  
Translational Termination

Late gene expression factors, LEF-4, LEF-8, LEF-9 and P47 constitute the primary components of the Autographa californica multinucleocapsid polyhedrovirus (AcMNPV)-encoded RNA polymerase, which initiates transcription from late and very late promoters. Here, characterization of lef-9 and lef-8, which encode their corresponding counterparts, from Bombyx mori NPV is reported. Transcription of lef-9 initiated at two independent sites: from a GCACT sequence located at −38 nt and a CTCTT sequence located at −50 nt, with respect to the +1 ATG of the open reading frame. The 3′ end of the transcript was mapped to a site 17 nt downstream of a canonical polyadenylation signal located 7 nt downstream of the first of the two tandem translational termination codons. Maximum synthesis of LEF-9 was seen from 36 h post-infection (p.i.). The transcription of lef-8 initiated early in infection from a GTGCAAT sequence that differed in the corresponding region from its AcMNPV counterpart (GCGCAGT), with consequent elimination of the consensus early transcription start site motif (underlined). Peak levels of lef-8 transcripts were attained by 24 h p.i. Immunocopurification analyses suggested that there was an association between LEF-8 and LEF-9 in vivo.

Characterization of late gene expression factor LEF-10 from Bombyx mori nucleopolyhedrovirus

2013 ◽  
Vol 175 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Wei Yu ◽  
Chao-Yi Du ◽  
Yan-Ping Quan ◽  
Zuo-Ming Nie ◽  
Jian Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Late Gene ◽  
Late Gene Expression ◽  
Bombyx Mori Nucleopolyhedrovirus

Bacteriophage T4 late gene expression: Overlapping promoters direct divergent transcription of the base plate gene cluster

Virology ◽  
1989 ◽  
Vol 171 (2) ◽  
pp. 475-483 ◽  
Author(s):  
Vincezo Scarlato ◽  
Aurora Storlazzi ◽  
Silvana Gargano ◽  
Antonino Cascino
Keyword(s):  
Gene Expression ◽  
Gene Cluster ◽  
Bacteriophage T4 ◽  
Base Plate ◽  
Late Gene ◽  
Late Gene Expression ◽  
Divergent Transcription
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