scholarly journals Cloning and characterization of Kluyveromyces lactis SEC14, a gene whose product stimulates Golgi secretory function in Saccharomyces cerevisiae.

1990 ◽  
Vol 172 (8) ◽  
pp. 4510-4521 ◽  
Author(s):  
S R Salama ◽  
A E Cleves ◽  
D E Malehorn ◽  
E A Whitters ◽  
V A Bankaitis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Kluyveromyces Lactis ◽  
Secretory Function ◽  
Golgi Secretory Function

scholarly journals Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: structural and functional relationships to GAL4 of Saccharomyces cerevisiae.

1987 ◽  
Vol 7 (3) ◽  
pp. 1111-1121 ◽  
Author(s):  
L V Wray ◽  
M M Witte ◽  
R C Dickson ◽  
M I Riley
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metal Binding ◽  
Glucose Repression ◽  
Kluyveromyces Lactis ◽  
Regulatory Gene ◽  
Regulatory Protein ◽  
Constitutive Expression ◽  
Functional Relationships ◽  
Functional Implications

Lactose or galactose induces the expression of the lactose-galactose regulon in Kluyveromyces lactis. We show here that the regulon is not induced in strains defective in LAC9. We demonstrate that this gene codes for a regulatory protein that acts in a positive manner to induce transcription. The LAC9 gene was isolated by complementation of a lac9 defective strain. DNA sequence analysis of the gene gave a deduced protein of 865 amino acids. Comparison of this sequence with that of the GAL4 protein of Saccharomyces cerevisiae revealed three regions of homology. One region of about 90 amino acid occurs at the amino terminus, which is known to mediate binding of GAL4 protein to upstream activator sequences. We speculate that a portion of this region, adjacent to the "metal-binding finger," specifies DNA binding. We discuss possible functions of the two other regions of homology. The functional implications of these structural similarities were examined. When LAC9 was introduced into a gal4 defective strain of S. cerevisiae it complemented the mutation and activated the galactose-melibiose regulon. However, LAC9 did not simply mimic GAL4. Unlike normal S. cerevisiae carrying GAL4, the strain carrying LAC9 gave constitutive expression of GAL1 and MEL1, two genes in the regulon. The strain did show glucose repression of the regulon, but repression was less severe with LAC9 than with GAL4. We discuss the implications of these results and how they may facilitate our understanding of the LAC9 and GAL4 regulatory proteins.

scholarly journals Characterization of the transcription factor encoding gene, KlADR1: metabolic role in Kluyveromyces lactis and expression in Saccharomyces cerevisiae

Microbiology ◽  
2016 ◽  
Vol 162 (11) ◽  
pp. 1933-1944 ◽  
Author(s):  
Silvia Cardarelli ◽  
Sirio D'Amici ◽  
Paola Tassone ◽  
Angela Tramonti ◽  
Daniela Uccelletti ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Kluyveromyces Lactis ◽  
Metabolic Role ◽  
Encoding Gene

Isolation and characterization of mutants of Saccharomyces cerevisiae Resistant to Killer Toxin of Kluyveromyces lactis

1990 ◽  
Vol 70 (4) ◽  
pp. 222-227 ◽  
Author(s):  
Shinichi Kawamoto ◽  
Naomi Arai ◽  
Masaaki Kobayashi ◽  
Kazumitsu Kawahara ◽  
Hitoshi Iwahashi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Kluyveromyces Lactis ◽  
Killer Toxin ◽  
Isolation And Characterization

Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: structural and functional relationships to GAL4 of Saccharomyces cerevisiae

1987 ◽  
Vol 7 (3) ◽  
pp. 1111-1121
Author(s):  
L V Wray ◽  
M M Witte ◽  
R C Dickson ◽  
M I Riley
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metal Binding ◽  
Glucose Repression ◽  
Kluyveromyces Lactis ◽  
Regulatory Gene ◽  
Regulatory Protein ◽  
Constitutive Expression ◽  
Functional Relationships ◽  
Functional Implications

Lactose or galactose induces the expression of the lactose-galactose regulon in Kluyveromyces lactis. We show here that the regulon is not induced in strains defective in LAC9. We demonstrate that this gene codes for a regulatory protein that acts in a positive manner to induce transcription. The LAC9 gene was isolated by complementation of a lac9 defective strain. DNA sequence analysis of the gene gave a deduced protein of 865 amino acids. Comparison of this sequence with that of the GAL4 protein of Saccharomyces cerevisiae revealed three regions of homology. One region of about 90 amino acid occurs at the amino terminus, which is known to mediate binding of GAL4 protein to upstream activator sequences. We speculate that a portion of this region, adjacent to the "metal-binding finger," specifies DNA binding. We discuss possible functions of the two other regions of homology. The functional implications of these structural similarities were examined. When LAC9 was introduced into a gal4 defective strain of S. cerevisiae it complemented the mutation and activated the galactose-melibiose regulon. However, LAC9 did not simply mimic GAL4. Unlike normal S. cerevisiae carrying GAL4, the strain carrying LAC9 gave constitutive expression of GAL1 and MEL1, two genes in the regulon. The strain did show glucose repression of the regulon, but repression was less severe with LAC9 than with GAL4. We discuss the implications of these results and how they may facilitate our understanding of the LAC9 and GAL4 regulatory proteins.

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae

1978 ◽  
Vol 525 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Shigeru Taketani ◽  
Takashi Osumi ◽  
Hirohiko Katsuki
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sterol Ester ◽  
Ester Hydrolase
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