scholarly journals Nucleotide sequence analysis reveals linked N-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of Streptomyces hygroscopicus.

1991 ◽  
Vol 173 (14) ◽  
pp. 4454-4463 ◽  
Author(s):  
A Raibaud ◽  
M Zalacain ◽  
T G Holt ◽  
R Tizard ◽  
C J Thompson
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Regulatory Genes ◽  
Streptomyces Hygroscopicus ◽  
Nucleotide Sequence Analysis ◽  
Biosynthetic Gene

scholarly journals Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant

2017 ◽  
Vol 30 (1) ◽  
pp. 172-174 ◽  
Author(s):  
Hiroya Ito ◽  
Sayaka Takahashi ◽  
Tetsuo Asai ◽  
Yutaka Tamura ◽  
Koshi Yamamoto
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Gene Cluster ◽  
Molecular Characterization ◽  
Dna Sequence ◽  
Actinobacillus Pleuropneumoniae ◽  
Nucleotide Sequence Analysis ◽  
Urease Gene ◽  
Negative Mutant

An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.

scholarly journals The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tü22: sequence analysis and expression in a heterologous host

1998 ◽  
Vol 5 (11) ◽  
pp. 647-659 ◽  
Author(s):  
Koji Ichinose ◽  
David J. Bedford ◽  
Diethild Tornus ◽  
Andreas Bechthold ◽  
Maureen J. Bibb ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Heterologous Host ◽  
Streptomyces Violaceoruber

scholarly journals In Vivo Analysis of the Regulatory Genes in the Nystatin Biosynthetic Gene Cluster of Streptomyces noursei ATCC 11455 Reveals Their Differential Control Over Antibiotic Biosynthesis

2004 ◽  
Vol 186 (5) ◽  
pp. 1345-1354 ◽  
Author(s):  
Olga N. Sekurova ◽  
Trygve Brautaset ◽  
Håvard Sletta ◽  
Sven E. F. Borgos ◽  
Øyvind M. Jakobsen ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Regulatory Genes ◽  
Antibiotic Biosynthesis ◽  
Biosynthetic Gene ◽  
Promoter Regions ◽  
Streptomyces Noursei ◽  
In Vivo Analysis ◽  
Differential Control

ABSTRACT Six putative regulatory genes are located at the flank of the nystatin biosynthetic gene cluster in Streptomyces noursei ATCC 11455. Gene inactivation and complementation experiments revealed that nysRI, nysRII, nysRIII, and nysRIV are necessary for efficient nystatin production, whereas no significant roles could be demonstrated for the other two regulatory genes. To determine the in vivo targets for the NysR regulators, chromosomal integration vectors with the xylE reporter gene under the control of seven putative promoter regions upstream of the nystatin structural and regulatory genes were constructed. Expression analyses of the resulting vectors in the S. noursei wild-type strain and regulatory mutants revealed that the four regulators differentially affect certain promoters. According to these analyses, genes responsible for initiation of nystatin biosynthesis and antibiotic transport were the major targets for regulation. Data from cross-complementation experiments showed that nysR genes could in some cases substitute for each other, suggesting a functional hierarchy of the regulators and implying a cascade-like mechanism of regulation of nystatin biosynthesis.

Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: Analysis of the enzymatic domains in the modular polyketide synthase

Gene ◽  
1996 ◽  
Vol 169 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Jesús F. Aparicio ◽  
István Molnár ◽  
Torsten Schwecke ◽  
Ariane König ◽  
Stephen F. Haydock ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Polyketide Synthase ◽  
Biosynthetic Gene Cluster ◽  
Streptomyces Hygroscopicus ◽  
Biosynthetic Gene ◽  
Modular Polyketide Synthase

scholarly journals Molecular cloning and sequence analysis of the complestatin biosynthetic gene cluster

2001 ◽  
Vol 98 (15) ◽  
pp. 8548-8553 ◽  
Author(s):  
H.-T. Chiu ◽  
B. K. Hubbard ◽  
A. N. Shah ◽  
J. Eide ◽  
R. A. Fredenburg ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Molecular Cloning ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene
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