Whole-Genome Sequencing of Streptomycin-Resistant Mycobacterium tuberculosis Strain SBH145 from Sabah, Malaysia

This paper reports on the whole-genome sequencing of a streptomycin-resistant Mycobacterium tuberculosis strain that was isolated from a patient with pulmonary tuberculosis in Sabah state of Malaysian Borneo. The strain belongs to the EAI2-Manila family of lineage 1 and is clustered with M. tuberculosis strains from the Philippines, India, and Taiwan.

Effect of Thymoquinone on Th1 and Th2 Balance in Rats Infected with Mycobacterium tuberculosis

Thymoquinone is an active compound in Nigella sativa which has potential immunomodulatory effect. Mycobacterium tuberculosis could alter the Th1 and Th2 balance by stimulating phagocyte IL-1β production, and subsequent Th2 differentiation. We aim to evaluate the effect of thymoquinone (TQ) in restore the Th1 and Th2 balance in Mycobacterium tuberculosis infection. Four groups of rats were infected with virulent Mycobacterium tuberculosis strain H37Rv. Thymoquinone at different doses was given to three groups, and one group left without treatment. Additional one group was either infected or treated with TQ. We measure IL-1β, IL-4 and IFN-γ levels using ELISA 14 days after TQ treatment. We found there were increased IL-1β, IL-4 and IFN-γ level after Mycobacterium tuberculosis infection, but we observe no significant effect of TQ treatment to Th1 and Th2 balance. We conclude that TQ could not restore Th1 and Th2 balance in rats infected with Mycobacterium tuberculosis.

AUTOMATED RAPID IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS STRAIN

—TB is curable and preventable. TB prevalence among men was over 2 times as high as among women. Actual number of TB infected people is much greater than notified and this problem is due sheer lack of accessibility to test facilities. we aim to design a setup that can be carried to remote areas basically slums where TB care facilities are inaccessible which will identify the presence of a Mycobacterium tuberculosis strain from a swab sample. The result will lead to into urgent actions to be taken for the treatment of the patient. The whole process will be automated and fast enough to give results in just a couple of minutes.

Synthesis of 2-Hydroxynaphthyl Pyrazolines Containing Isoniazid Moiety: A Potential Antitubercular Agents

: The new series of pyrazolines derivatives containing isoniazid moiety were synthesized from 2-hydroxynaphthyl functionalized chalcones and isoniazid using sodium hydroxide as a base in 2-ethoxy ethanol.We are evaluated for their antitubercular activity against Mycobacterium tuberculosis strain (H37Rv) by Microplate Alamar Blue Assay (MABA) some of the tested compounds 3a, 3b, and 3c, were found to have higher antitubercular activity than the selected standard drugs, where as the compounds 3d, 3e, 3i and 3j found to have higher antitubercular activity than Streptomycin and same as that of drug Pyrazinamide and Ciprofloxacin. While remaining compound showed moderate activity. Where as it is found that the disubstituted halogen compound and electron withdrawing group on the phenyl ring are important substitution for increase in antitubercular activity.