Messenger RNA secondary structure and translational coupling in the Escherichia coli operon encoding translation initiation factor IF3 and the ribosomal proteins, L35 and L20

1992 ◽  
Vol 228 (2) ◽  
pp. 366-386 ◽  
Author(s):  
P. Lesage ◽  
C. Chiaruttini ◽  
M. Graffe ◽  
J. Dondon ◽  
M. Milet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Translation Initiation ◽  
Messenger Rna ◽  
Rna Secondary Structure ◽  
Ribosomal Proteins ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Translational Coupling

scholarly journals OseIF3h Regulates Plant Growth and Pollen Development at Translational Level Presumably through Interaction with OsMTA2

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1101
Author(s):  
Yuqing Huang ◽  
Peng Zheng ◽  
Xuejiao Liu ◽  
Hao Chen ◽  
Jumin Tu
Keyword(s):  
Plant Growth ◽  
Translation Initiation ◽  
Pollen Development ◽  
Messenger Rna ◽  
Protein Biosynthesis ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Sequencing Data ◽  
Knock Out ◽  
Translational Level

The initiation stage of protein biosynthesis is a sophisticated process tightly regulated by numerous initiation factors and their associated components. However, the mechanism underlying translation initiation has not been completely understood in rice. Here, we showed knock-out mutation of the rice eukaryotic translation initiation factor 3 subunit h (OseIF3h) resulted in plant growth retardation and seed-setting rate reduction as compared to the wild type. Further investigation demonstrated an interaction between OseIF3h and OsMTA2 (mRNA adenosine methylase 2), a rice homolog of METTL3 (methyltransferase-like 3) in mammals, which provided new insight into how N6-methyladenosine (m6A) modification of messenger RNA (mRNA) is engaged in the translation initiation process in monocot species. Moreover, the RIP-seq (RNA immunoprecipitation sequencing) data suggested that OseIF3h was involved in multiple biological processes, including photosynthesis, cellular metabolic process, precursor metabolites, and energy generation. Therefore, we infer that OseIF3h interacts with OsMTA2 to target a particular subset of genes at translational level, regulating plant growth and pollen development.

Solution Structure of C-Terminal Escherichia coli Translation Initiation Factor IF2 by Small-Angle X-ray Scattering†

Biochemistry ◽  
2008 ◽  
Vol 47 (20) ◽  
pp. 5590-5598 ◽  
Author(s):  
Louise Carøe Vohlander Rasmussen ◽  
Cristiano Luis Pinto Oliveira ◽  
Janni Mosgaard Jensen ◽  
Jan Skov Pedersen ◽  
Hans Uffe Sperling-Petersen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Small Angle ◽  
Solution Structure ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

scholarly journals Essential role for an isoform of Escherichia coli translation initiation factor IF2 in repair of two-ended DNA double-strand breaks

2021 ◽  
Author(s):  
Jillella Mallikarjun ◽  
J Gowrishankar
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Double Strand Breaks ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Dna Double Strand Breaks ◽  
Chromosomal Locus ◽  
Strand Breaks ◽  
Damage Repair ◽  
Replication Restart

In Escherichia coli, three isoforms of the essential translation initiation factor IF2 (IF2-1, IF2-2, and IF2-3) are generated from separate in-frame initiation codons in infB. The isoforms have earlier been suggested to additionally participate in DNA damage repair and replication restart. It is also known that the proteins RecA and RecBCD are needed for repair of DNA double-strand breaks (DSBs) in E. coli. Here we show that strains lacking IF2-1 are profoundly sensitive to two-ended DSBs in DNA generated by radiomimetic agents phleomycin or bleomycin, or by endonuclease I-SceI. However, these strains remained tolerant to other DSB-generating genotoxic agents or perturbations to which recA and recBC mutants remained sensitive, such as to mitomycin C, type-2 DNA topoisomerase inhibitors, or DSB caused by palindrome cleavage behind a replication fork. Data from genome-wide copy number analyses following I-SceI cleavage at a single chromosomal locus suggested that, in a strain lacking IF2-1, the magnitude of break induced replication through replication restart mechanisms is largely preserved but the extent of DNA resection around the DSB site is reduced. We propose that in absence of IF2-1 it is the annealing of a RecA nucleoprotein filament to its homologous target that is weakened, which in turn leads to a specific failure in assembly of Ter-to-oriC directed replisomes needed for consummation of two-ended DSB repair.

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