scholarly journals Cloning, Sequencing, and Characterization of the recAGene from Rhodopseudomonas viridis and Construction of arecA Strain

1998 ◽  
Vol 180 (12) ◽  
pp. 3227-3232 ◽  
Author(s):  
I-Peng Chen ◽  
Hartmut Michel
Keyword(s):  
Dna Damage ◽  
Rhizobium Meliloti ◽  
Photosynthetic Reaction Center ◽  
Reaction Centers ◽  
Photosynthetic Reaction Centers ◽  
Rhodopseudomonas Viridis ◽  
High Identity ◽  
Homologous Expression ◽  
Increased Sensitivity

ABSTRACT A recombination-deficient strain of the phototrophic bacteriumRhodopseudomonas viridis was constructed for the homologous expression of modified photosynthetic reaction center genes. TheR. viridis recA gene was cloned and subsequently deleted from the R. viridis genome. The clonedR. viridis recA gene shows high identity to knownrecA genes and was able to complement the Rec−phenotype of a Rhizobium meliloti recA strain. The constructed R. viridis recA strain showed the general Rec− phenotype, i.e., increased sensitivity to DNA damage and severely impaired recombination ability. The latter property of this strain will be of advantage in particular for expression of modified, nonfunctional photosynthetic reaction centers which are not as yet available.

Characterization of photosynthetic reaction centers by surface-enhanced resonance Raman scattering

1993 ◽  
Author(s):  
George D. Chumanov ◽  
Therese M. Cotton ◽  
Chengli Zhou ◽  
Dale Gaul ◽  
Rafael Picorel ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Resonance Raman ◽  
Reaction Centers ◽  
Photosynthetic Reaction Centers ◽  
Resonance Raman Scattering ◽  
Surface Enhanced

Reaction of cytochrome c2 with photosynthetic reaction centers from Rhodopseudomonas viridis

Biochemistry ◽  
1991 ◽  
Vol 30 (5) ◽  
pp. 1303-1310 ◽  
Author(s):  
David B. Knaff ◽  
Anne Willie ◽  
Joan E. Long ◽  
Aidas Kriauciunas ◽  
Bill Durham ◽  
...  
Keyword(s):  
Reaction Centers ◽  
Photosynthetic Reaction Centers ◽  
Rhodopseudomonas Viridis ◽  
Cytochrome C2

scholarly journals Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells

2017 ◽  
Vol 114 (15) ◽  
pp. 3837-3842 ◽  
Author(s):  
Emiliano Altamura ◽  
Francesco Milano ◽  
Roberto R. Tangorra ◽  
Massimo Trotta ◽  
Omar Hassan Omar ◽  
...  
Keyword(s):  
Reaction Center ◽  
Lipid Membrane ◽  
Transmembrane Protein ◽  
Photosynthetic Reaction Center ◽  
Lipid Vesicles ◽  
Reaction Centers ◽  
Proton Gradient ◽  
Continuous Illumination ◽  
Photosynthetic Reaction Centers ◽  
Transfer Method

Photosynthesis is responsible for the photochemical conversion of light into the chemical energy that fuels the planet Earth. The photochemical core of this process in all photosynthetic organisms is a transmembrane protein called the reaction center. In purple photosynthetic bacteria a simple version of this photoenzyme catalyzes the reduction of a quinone molecule, accompanied by the uptake of two protons from the cytoplasm. This results in the establishment of a proton concentration gradient across the lipid membrane, which can be ultimately harnessed to synthesize ATP. Herein we show that synthetic protocells, based on giant lipid vesicles embedding an oriented population of reaction centers, are capable of generating a photoinduced proton gradient across the membrane. Under continuous illumination, the protocells generate a gradient of 0.061 pH units per min, equivalent to a proton motive force of 3.6 mV⋅min−1. Remarkably, the facile reconstitution of the photosynthetic reaction center in the artificial lipid membrane, obtained by the droplet transfer method, paves the way for the construction of novel and more functional protocells for synthetic biology.

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