Spectrophotometric and voltage clamp characterization of monolayers of bacterial photosynthetic reaction centers

1982 ◽  
Vol 681 (2) ◽  
pp. 191-201 ◽  
Author(s):  
David M. Tiede ◽  
Paul Mueller ◽  
P.Leslie Dutton
1993 ◽  
Author(s):  
George D. Chumanov ◽  
Therese M. Cotton ◽  
Chengli Zhou ◽  
Dale Gaul ◽  
Rafael Picorel ◽  
...  

Biochemistry ◽  
1997 ◽  
Vol 36 (24) ◽  
pp. 7513-7519 ◽  
Author(s):  
Tatjana A. Egorova-Zachernyuk ◽  
Barth van Rossum ◽  
Gert-Jan Boender ◽  
Eric Franken ◽  
Jennifer Ashurst ◽  
...  

1998 ◽  
Vol 180 (12) ◽  
pp. 3227-3232 ◽  
Author(s):  
I-Peng Chen ◽  
Hartmut Michel

ABSTRACT A recombination-deficient strain of the phototrophic bacteriumRhodopseudomonas viridis was constructed for the homologous expression of modified photosynthetic reaction center genes. TheR. viridis recA gene was cloned and subsequently deleted from the R. viridis genome. The clonedR. viridis recA gene shows high identity to knownrecA genes and was able to complement the Rec−phenotype of a Rhizobium meliloti recA strain. The constructed R. viridis recA strain showed the general Rec− phenotype, i.e., increased sensitivity to DNA damage and severely impaired recombination ability. The latter property of this strain will be of advantage in particular for expression of modified, nonfunctional photosynthetic reaction centers which are not as yet available.


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