scholarly journals Gas Channels for NH3: Proteins from Hyperthermophiles Complement an Escherichia coli Mutant

2002 ◽  
Vol 184 (12) ◽  
pp. 3396-3400 ◽  
Author(s):  
Eric Soupene ◽  
Tony Chu ◽  
Rebecca W. Corbin ◽  
Donald F. Hunt ◽  
Sydney Kustu
Keyword(s):  
Escherichia Coli ◽  
Affinity Chromatography ◽  
Membrane Transport ◽  
Transport Proteins ◽  
Ammonium Transport ◽  
Aquifex Aeolicus ◽  
Membrane Transport Proteins ◽  
Nickel Chelate ◽  
Mesophilic Bacterium ◽  
Low Expression

ABSTRACT Ammonium transport (Amt) proteins appear to be bidirectional channels for NH3. The amt genes of the hyperthermophiles Aquifex aeolicus and Methanococcus jannaschii complement enteric amtB mutants for growth at 25 nM NH3 at 37°C. To our knowledge, Amt proteins are the first hyperthermophilic membrane transport proteins shown to be active in a mesophilic bacterium. Despite low expression levels, His-tagged Aquifex Amt could be purified by heating and nickel chelate affinity chromatography. It could be studied genetically in Escherichia coli.

scholarly journals An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e76913 ◽  
Author(s):  
Pikyee Ma ◽  
Filipa Varela ◽  
Malgorzata Magoch ◽  
Ana Rita Silva ◽  
Ana Lúcia Rosário ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Transport ◽  
Transport Proteins ◽  
Small Scale ◽  
Efficient Strategy ◽  
Membrane Transport Proteins

Expression of prokaryotic membrane transport proteins in Escherichia coli

1999 ◽  
Vol 27 (6) ◽  
pp. 893-899 ◽  
Author(s):  
A. Ward ◽  
J. O'Reilly ◽  
N. G. Rutherford ◽  
S. M. Ferguson ◽  
C. K. Hoyle ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Transport ◽  
Transport Proteins ◽  
Membrane Transport Proteins
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