PII T-Loop Mutations Affecting Signal Transduction to NtrB Also Abolish Yeast Two-Hybrid Interactions
2002 ◽
Vol 184
(13)
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pp. 3746-3748
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ABSTRACT Mutations A49P and Δ47-53 at the T loop of the Escherichia coli GlnB (PII) protein impair regulatory interactions with the two-component sensor regulator NtrB (P. Jiang, P. Zucker, M. R. Atkinson, E. S. Kamberov, W. Tirasophon, P. Chandran, B. R. Schepke, and A. J. Ninfa, J. Bacteriol. 179: 4342-4353, 1997). We show here that these mutations also impair interactions between PII and NtrB in the yeast two-hybrid system, indicating that defects in NtrB regulation closely reflect binding impairment. The reported results underline the strength of two-hybrid assays for analysis of interactions involving the T loop of PII proteins.
2003 ◽
Vol 185
(8)
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pp. 2393-2401
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Interactions of the F1-ATPase subunits from Escherichia coli detected by the yeast two-hybrid system
1996 ◽
Vol 1274
(1-2)
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pp. 67-72
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Keyword(s):
2003 ◽
Vol 269
(4)
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pp. 574-581
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Keyword(s):
Keyword(s):