scholarly journals Use of Multiple-Displacement Amplification and Checkerboard DNA-DNA Hybridization To Examine the Microbiota of Endodontic Infections

2007 ◽  
Vol 45 (9) ◽  
pp. 3039-3049 ◽  
Author(s):  
L. C. N. Brito ◽  
F. R. Teles ◽  
R. P. Teles ◽  
E. C. Franca ◽  
A. P. Ribeiro-Sobrinho ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Multiple Displacement Amplification ◽  
Endodontic Infections

Evaluation of the microbiota of primary endodontic infections using checkerboard DNA–DNA hybridization

2007 ◽  
Vol 22 (6) ◽  
pp. 390-397 ◽  
Author(s):  
L. Sassone ◽  
R. Fidel ◽  
L. Figueiredo ◽  
S. Fidel ◽  
M. Faveri ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Endodontic Infections

A Microbiological Profile of Unexposed and Exposed Pulp Space of Primary Endodontic Infections by Checkerboard DNA-DNA Hybridization

2012 ◽  
Vol 38 (7) ◽  
pp. 889-893 ◽  
Author(s):  
Luciana M. Sassone ◽  
Rivail A. Fidel ◽  
Marcelo Faveri ◽  
Luciene Figueiredo ◽  
Sandra R. Fidel ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Microbiological Profile ◽  
Endodontic Infections

scholarly journals Microbiota of deciduous endodontic infections analysed by MDA and Checkerboard DNA-DNA hybridization

2010 ◽  
Vol 44 (3) ◽  
pp. 225-235 ◽  
Author(s):  
W. L. F. Tavares ◽  
L. C. Neves de Brito ◽  
R. P. Teles ◽  
M. L. A. Massara ◽  
A. P. Ribeiro Sobrinho ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Endodontic Infections

scholarly journals Searching for Helicobacter pylori and Chlamydia pneumoniae in primary endodontic infections

2012 ◽  
Vol 06 (02) ◽  
pp. 158-162 ◽  
Author(s):  
Isabela N Rôças ◽  
José F Siqueira
Keyword(s):  
Helicobacter Pylori ◽  
Chlamydia Pneumoniae ◽  
Multiple Displacement Amplification ◽  
Clinical Sample ◽  
Clinical Samples ◽  
Human Pathogens ◽  
Root Canals ◽  
Endodontic Infections ◽  
H Pylori ◽  
Species Specific

ABSTRACTObjectives: The purpose of this study was to search samples from primary endodontic infections for the presence of two common human bacterial pathogens -Helicobacter pylori and Chlamydia pneumoniaeMethods: Genomic DNA isolated from samples taken from 25 root canals of teeth with asymptomatic (chronic) apical periodontitis and 25 aspirates from acute apical abscess was initially amplified by the multiple displacement amplification approach and then used as template in species-specific polymerase chain reaction (PCR) for detection of H. pylori and C. pneumoniae. Results: All clinical samples were positive for the presence of bacterial DNA. However, no clinical sample was positive for either H. pylori or C. pneumoniaeConclusions: Neither H. pylori nor C. pneumoniae were found in samples from primary endodontic infections. These findings suggest that these species are not candidate endodontic pathogens and that the necrotic root canal does not serve as a reservoir for these human pathogens in healthy patients. (Eur J Dent 2012;6:158-162)

Checkerboard DNA-DNA hybridization analysis of endodontic infections

2000 ◽  
Vol 89 (6) ◽  
pp. 744-748 ◽  
Author(s):  
José F. Siqueira ◽  
Isabela N. Rôças ◽  
Renata Souto ◽  
Milton de Uzeda ◽  
Ana Paula Colombo
Keyword(s):  
Dna Hybridization ◽  
Hybridization Analysis ◽  
Endodontic Infections
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