Coproantibodies in hepatitis A: detection by enzyme-linked immunosorbent assay and immune electron microscopy.

1980 ◽  
Vol 11 (6) ◽  
pp. 710-716 ◽  
Author(s):  
S A Locarnini ◽  
A G Coulepis ◽  
J Kaldor ◽  
I D Gust
Keyword(s):  
Electron Microscopy ◽  
Immunosorbent Assay ◽  
Hepatitis A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Electron Microscopy

An Outbreak of Hepatitis A Investigated by Immune Electron Microscopy and Enzyme-Linked Immunosorbent Assay

1979 ◽  
Vol 11 (1) ◽  
pp. 97-98 ◽  
Author(s):  
Bertil Tufvesson ◽  
Joke Van Der Veen ◽  
Willem Duermeyer ◽  
Per-Magnus Niklasson ◽  
Torsten Johnsson
Keyword(s):  
Electron Microscopy ◽  
Immunosorbent Assay ◽  
Hepatitis A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Electron Microscopy

scholarly journals Enzyme-Linked Immunosorbent Assay for Detection of Hepatitis A Antigen in Stool and Antibody to Hepatitis A Antigen in Sera: Comparison with Solid-Phase Radioimmunoassay, Immune Electron Microscopy, and Immune Adherence Hemagglutination Assay

1978 ◽  
Vol 7 (2) ◽  
pp. 184-193
Author(s):  
Lars R. Mathiesen ◽  
Stephen M. Feinstone ◽  
Doris C. Wong ◽  
Peter Skinhoej ◽  
Robert H. Purcell
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Electron Microscopy ◽  
Specific Detection ◽  
Immune Adherence ◽  
Ag Particles ◽  
Solid Phase Radioimmunoassay ◽  
Human Stool

Previously described techniques for detection of hepatitis A antigen (HA Ag) and antibody (anti-HA) have required purified HA Ag and expensive equipment. Herein is described an enzyme-linked immunosorbent assay (ELISA) for specific detection of HA Ag in human stool filtrates and of anti-HA in sera by using selected HA Ag-containing human stool filtrates as the antigen source. Because human stools often react nonspecifically in serological tests for HA Ag, blocking with preexposure and hyperimmune anti-HA sera from a chimpanzee inoculated with hepatitis A virus was used to confirm specific detection of HA Ag. The sensitivity of ELISA was found to be comparable to that of solid-phase radioimmunoassay (SPRIA) and immune electron microscopy (IEM). Of 37 acute-phase stools collected from nine patients, 16 were positive for HA Ag by ELISA. In 13 of these, HA Ag particles were found by IEM, and an additional 3 stools negative by ELISA contained HA Ag particles by IEM. Eight control stools were negative by both ELISA and IEM. Anti-HA was measured in sera by demonstrating its ability to block binding of the enzyme conjugate to HA Ag in a stool without detectable nonspecificity. This test (blocking ELISA) was as sensitive and specific as blocking SPIRA, IEM, and immune adherence hemagglutination and, like SPRIA and IEM, detected early-developing antibody. The ELISA is simple to perform and requires only a minimum of equipment. It is useful for screening stools for HA Ag and for monitoring HA Ag during purification, as well as for detecting early and late anti-HA in sera.

scholarly journals Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A virus

1978 ◽  
Vol 8 (3) ◽  
pp. 277-282 ◽  
Author(s):  
S A Locarnini ◽  
S M Garland ◽  
N I Lehmann ◽  
R C Pringle ◽  
I D Gust
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Field Work ◽  
Enzyme Linked Immunosorbent Assay ◽  
Technical Equipment ◽  
Immune Electron Microscopy ◽  
Naked Eye ◽  
Household Contacts

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of hepatitis A virus in human fecal specimens. Investigations with 88 fecal specimens from 77 patients with suspected viral hepatitis and 8 of their household contacts showed that ELISA was as specific and sensitive as radioimmunoassay and almost as sensitive as immune electron microscopy. The ELISA is quick and simple to perform, does not require sophisticated technical equipment, and can be read with the naked eye, making it suitable for field work and rapid diagnosis.

Sign in / Sign up

Export Citation Format

Share Document