Large-scale purification of hepatitis B surface antigen

Journal of Clinical Microbiology ◽

10.1128/jcm.3.6.626-631.1976 ◽

1976 ◽

Vol 3 (6) ◽

pp. 626-631

Author(s):

J Vnek ◽

A M Prince

Keyword(s):

Polyethylene Glycol ◽

Hepatitis B ◽

Surface Antigen ◽

Phosphate Buffer ◽

Sodium Phosphate ◽

Large Scale ◽

Hepatitis B Surface Antigen ◽

Sodium Phosphate Buffer ◽

Subsequent Elution ◽

Polyethylene Glycol Precipitation

Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.

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Large-scale purification of recombinant hepatitis B surface antigen from Pichia pastoris with non-affinity chromatographic methods as a substitute to immunoaffinity chromatography

Preparative Biochemistry & Biotechnology ◽

10.1080/10826068.2018.1487854 ◽

2018 ◽

Vol 48 (8) ◽

pp. 683-692 ◽

Author(s):

Seyed Nezamedin Hosseini ◽

Amin Javidanbardan ◽

Behnaz Sadat Alizadeh Salim ◽

Maryam Khatami

Keyword(s):

Pichia Pastoris ◽

Hepatitis B ◽

Surface Antigen ◽

Large Scale ◽

Hepatitis B Surface Antigen ◽

Immunoaffinity Chromatography ◽

Recombinant Hepatitis ◽

Chromatographic Methods

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Hepatitis B-surface antigen (HBsAg) vaccine fabricated chitosan-polyethylene glycol nanocomposite (HBsAg-CS-PEG- NC) preparation, immunogenicity, controlled release pattern, biocompatibility or non-target toxicity

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2019.09.175 ◽

2020 ◽

Vol 144 ◽

pp. 978-994 ◽

Author(s):

Karthick Raja Namasivayam S ◽

AN Nishanth ◽

Arvind Bharani R S ◽

Kiran Nivedh ◽

Nawaz Hussain Syed ◽

...

Keyword(s):

Polyethylene Glycol ◽

Controlled Release ◽

Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Release Pattern

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Purification of Hepatitis B Surface Antigen Using Polyethylene Glycol, Pepsin and Tween 80

Vox Sanguinis ◽

10.1159/000467370 ◽

1976 ◽

Vol 31 (1) ◽

pp. 84-95

Author(s):

Nrapendra Nath ◽

Scott Mazzur ◽

Robert Ledman ◽

Chyang T. Fang

Keyword(s):

Polyethylene Glycol ◽

Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

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Large-scale isolation of the two major polypeptides of the hepatitis B surface antigen (HBsAg) by gel filtration in 6 M guanidinium chloride

Journal of Virological Methods ◽

10.1016/0166-0934(82)90039-8 ◽

1982 ◽

Vol 3 (6) ◽

pp. 349-354

Author(s):

Michel Hubert ◽

Francis Barin ◽

Alain Goudeau

Keyword(s):

Hepatitis B ◽

Surface Antigen ◽

Large Scale ◽

Hepatitis B Surface Antigen ◽

Gel Filtration ◽

Guanidinium Chloride

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Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2003.08.149 ◽

2003 ◽

Vol 310 (3) ◽

pp. 742-747 ◽

Author(s):

Rodolfo Valdés ◽

Biunayki Reyes ◽

Tatiana Alvarez ◽

Janet Garcı́a ◽

José A Montero ◽

...

Keyword(s):

Hepatitis B ◽

Surface Antigen ◽

Specific Antibody ◽

Large Scale ◽

Hepatitis B Surface Antigen

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Purification of Hepatitis B Surface Antigen Using Polyethylene Glycol, Pepsin and Tween 80

Vox Sanguinis ◽

10.1111/j.1423-0410.1976.tb02226.x ◽

1976 ◽

Vol 31 ◽

pp. 84-95 ◽

Author(s):

Nrapendra Nath ◽

Scorn Mazzur ◽

Robert Ledman ◽

Chyang T. Fang

Keyword(s):

Polyethylene Glycol ◽

Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

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Assessing virus like particles formation and r-HBsAg aggregation during large scale production of recombinant hepatitis B surface antigen from Pichia pastoris

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2019.08.019 ◽

2019 ◽

Vol 139 ◽

pp. 697-711

Author(s):

Seyed Nezamedin Hosseini ◽

Taravat Sarvari ◽

Ghazal Bashiri ◽

Maryam Khatami ◽

Seyed Abbas Shojaosadati

Keyword(s):

Pichia Pastoris ◽

Hepatitis B ◽

Surface Antigen ◽

Large Scale ◽

Hepatitis B Surface Antigen ◽

Scale Production ◽

Recombinant Hepatitis ◽

Virus Like Particles ◽

Large Scale Production

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Improving the recovery of clarification process of recombinant hepatitis B surface antigen in large-scale by optimizing adsorption-desorption parameters on Aerosil-380

Preparative Biochemistry & Biotechnology ◽

10.1080/10826068.2018.1466153 ◽

2018 ◽

Vol 48 (6) ◽

pp. 490-497 ◽

Author(s):

Seyed Nezamedin Hosseini ◽

Parisa Ghaisari ◽

Shahram Sharifnia ◽

Maryam Khatami ◽

Amin Javidanbardan

Keyword(s):

Hepatitis B ◽

Surface Antigen ◽

Large Scale ◽

Hepatitis B Surface Antigen ◽

Recombinant Hepatitis ◽

Adsorption Desorption

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Enhancing recovery of recombinant hepatitis B surface antigen in lab-scale and large-scale anion-exchange chromatography by optimizing the conductivity of buffers

Protein Expression and Purification ◽

10.1016/j.pep.2017.08.011 ◽

2018 ◽

Vol 141 ◽

pp. 25-31 ◽

Author(s):

Gol Mohammad Mojarrad Moghanloo ◽

Maryam Khatami ◽

Amin Javidanbardan ◽

Seyed Nezamedin Hosseini

Keyword(s):

Hepatitis B ◽

Anion Exchange ◽

Surface Antigen ◽

Large Scale ◽

Hepatitis B Surface Antigen ◽

Anion Exchange Chromatography ◽

Exchange Chromatography ◽

Recombinant Hepatitis

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Large-scale production of recombinant hepatitis B surface antigen from Pichia pastoris

Journal of Biotechnology ◽

10.1016/s0168-1656(99)00201-1 ◽

2000 ◽

Vol 77 (2-3) ◽

pp. 157-167 ◽

Author(s):

Eugenio Hardy ◽

Eduardo Martı́nez ◽

David Diago ◽

Raúl Dı́az ◽

Daniel González ◽

...

Keyword(s):

Pichia Pastoris ◽

Hepatitis B ◽

Surface Antigen ◽

Large Scale ◽

Hepatitis B Surface Antigen ◽

Scale Production ◽

Recombinant Hepatitis ◽

Large Scale Production

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