Large-scale isolation of the two major polypeptides of the hepatitis B surface antigen (HBsAg) by gel filtration in 6 M guanidinium chloride

1982 ◽  
Vol 3 (6) ◽  
pp. 349-354
Author(s):  
Michel Hubert ◽  
Francis Barin ◽  
Alain Goudeau
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Large Scale ◽  
Hepatitis B Surface Antigen ◽  
Gel Filtration ◽  
Guanidinium Chloride

Synthesis of hepatitis B surface antigen pre-S2 region fragments and studies on their immunogenicity

1988 ◽  
Vol 53 (11) ◽  
pp. 2952-2956 ◽  
Author(s):  
Bernard Lammek ◽  
Zbigniew Maćkiewicz ◽  
Izabela Derdowska ◽  
Hanna Świderska ◽  
Adam Nowosławski ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Antigenic Determinants ◽  
Phase Method ◽  
Peptide Fragments ◽  
Humoral Immune

Two peptide fragments of hepatitis B surface antigen pre-S2 region were synthesized by the solid phase method. The peptides were purified by gel filtration or ion-exchange chromatography on Sephadex SP-C-25. Both peptides induced a cellular and humoral immune response in rabbits. The results showed that fragment 14-22 of pre-S2 region contains one of the antigenic determinants.

scholarly journals Large-scale purification of hepatitis B surface antigen

1976 ◽  
Vol 3 (6) ◽  
pp. 626-631
Author(s):  
J Vnek ◽  
A M Prince
Keyword(s):  
Polyethylene Glycol ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Phosphate Buffer ◽  
Sodium Phosphate ◽  
Large Scale ◽  
Hepatitis B Surface Antigen ◽  
Sodium Phosphate Buffer ◽  
Subsequent Elution ◽  
Polyethylene Glycol Precipitation

Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.

Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale

2003 ◽  
Vol 310 (3) ◽  
pp. 742-747 ◽  
Author(s):  
Rodolfo Valdés ◽  
Biunayki Reyes ◽  
Tatiana Alvarez ◽  
Janet Garcı́a ◽  
José A Montero ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Specific Antibody ◽  
Large Scale ◽  
Hepatitis B Surface Antigen

Large-scale production of recombinant hepatitis B surface antigen from Pichia pastoris

2000 ◽  
Vol 77 (2-3) ◽  
pp. 157-167 ◽  
Author(s):  
Eugenio Hardy ◽  
Eduardo Martı́nez ◽  
David Diago ◽  
Raúl Dı́az ◽  
Daniel González ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Large Scale ◽  
Hepatitis B Surface Antigen ◽  
Scale Production ◽  
Recombinant Hepatitis ◽  
Large Scale Production

scholarly journals Evaluation of Four Rapid Tests for Detection of Hepatitis B Surface Antigen in Ivory Coast

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Bamory Dembele ◽  
Roseline Affi-Aboli ◽  
Mathieu Kabran ◽  
Daouda Sevede ◽  
Vanessa Goha ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Whole Blood ◽  
Ivory Coast ◽  
Large Scale ◽  
Hepatitis B Surface Antigen ◽  
False Negative ◽  
Evaluation Study ◽  
Hbv Infection ◽  
Cross Sectional

Background. Hepatitis B virus (HBV) infection is a leading cause of liver disease worldwide. Hepatitis B surface antigen (HBsAg) rapid diagnostic tests (RDTs) could be an ideal tool for a large-scale HBV screening in settings with high endemicity but limited infrastructure. The aim of this study was to evaluate the diagnosis performance of such RDTs for screening HBV infection in Ivory Coast. Methods. From September 2018 to January 2019, a cross-sectional phase I evaluation study of RDTs was conducted in three laboratories of Abidjan (CeDReS, CNTS and IPCI), on a panel of 405 whole blood samples and 699 plasmas. Four HBsAg RDTs (Determine™ HBsAg, SD Bioline HBsAg WB®, Standard Q HBsAg® and Vikia HBsAg®) were evaluated. The diagnostic performance (sensitivity and specificity) was calculated in comparison to the reference sequential algorithms of two EIA tests (Dia.Pro HBsAg® one version ULTRA and Monolisa™ HBsAg ULTRA). Results. The Determine™ HBsAg and Vikia HBsAg® tests performed well, with 100% of sensitivity, specificity both on plasma and on whole blood. For SD Bioline HBsAg WB® and Standard Q HBsAg®, the specificities were 99.8% and the sensitivities 99.3% and 97.1% respectively. Finally, there were a total of 19 false negative results: 3 with SD Bioline HBsAg WB® and 16 with Standard Q HBsAg®. Conclusion. Determine HBsAg® from Alere and Vikia HBsAg® from Biomérieux are the most suitable RDTs for screening for HBV in Ivory Coast. A phase II evaluation must be initiated.

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