Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction.

1992 ◽  
Vol 30 (5) ◽  
pp. 1189-1193 ◽  
Author(s):  
C Le Bouguenec ◽  
M Archambaud ◽  
A Labigne
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Uropathogenic Escherichia Coli ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

2019 ◽  
Vol 19 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Hassan Valadbeigi ◽  
Elham Esmaeeli ◽  
Sobhan Ghafourian ◽  
Abbas Maleki ◽  
Nourkhoda Sadeghifard
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multiplex Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Uropathogenic Escherichia Coli ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

Expression and detection of pap-, sfa-, and afa-encoded fimbrial adhesin systems among uropathogenic Escherichia coli

1994 ◽  
Vol 40 (4) ◽  
pp. 286-291 ◽  
Author(s):  
France Daigle ◽  
Josée Harel ◽  
John M. Fairbrother ◽  
Pierre Lebel
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Asymptomatic Bacteriuria ◽  
Uropathogenic Escherichia Coli ◽  
Class Iii ◽  
Chain Reaction ◽  
Hemolysin Gene ◽  
Polymerase Chain ◽  
Positive Results ◽  
Fimbrial Adhesin

Thirty-five Escherichia coli isolates from young children and women with pyelonephritis, cystitis, or asymptomatic bacteriuria were characterized genotypically and phenotypically. The isolates were examined genotypically by using DNA probes specific for the hemolysin gene and for the pap, sfa, and afa adhesin systems. Genes for the adhesin systems were also detected by polymerase chain reaction, using multigene amplification. The isolates were serotyped, tested for hemolysin production, and classified for their adhesion specificity by hemagglutination and by binding specificity assays. Twenty-seven of the 35 isolates were pap positive. Results showed that pap-positive isolates expressing class I or class II G adhesins were more frequent in cases of pyelonephritis than in cases of cystitis and asymptomatic bacteriuria. Expression of the class III G adhesins was more frequent in isolates from cystitis and asymptomatic bacteriuria than in isolates from pyelonephritis. Multiple adhesin systems and hemolysin were more frequently found in isolates from cases of pyelonephritis than in isolates from cases of cystitis and asymptomatic bacteriuria. There was perfect correlation between the results obtained by polymerase chain reaction and and those obtained by hybridization.Key words: Escherichia coli, UTI, fimbriae, PCR, virulence factors.

Polymerase chain reaction for the specific detection of Escherichia coli/Shigella

1993 ◽  
Vol 144 (7) ◽  
pp. 557-564 ◽  
Author(s):  
G Spierings ◽  
C Ockhuijsen ◽  
H Hofstra ◽  
J Tommassen
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain
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