scholarly journals Rapid differentiation of Mycobacterium avium and M. paratuberculosis by PCR and restriction enzyme analysis.

1996 ◽  
Vol 34 (3) ◽  
pp. 734-737 ◽  
Author(s):  
I S Eriks ◽  
K T Munck ◽  
T E Besser ◽  
G H Cantor ◽  
V Kapur
Keyword(s):  
Mycobacterium Avium ◽  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis

scholarly journals Identificação de micobactérias não tuberculosas isoladas de sítios estéreis em pacientes em um hospital universitário na cidade do Rio de Janeiro

2011 ◽  
Vol 37 (4) ◽  
pp. 521-526 ◽  
Author(s):  
Simone Gonçalves Senna ◽  
Ana Grazia Marsico ◽  
Gisele Betzler de Oliveira Vieira ◽  
Luciana Fonseca Sobral ◽  
Philip Noel Suffys ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Restriction Enzyme ◽  
Rio De Janeiro ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis

OBJETIVO: Identificar micobactérias não tuberculosas (MNT) isoladas de sítios estéreis em pacientes internados no Hospital Universitário Clementino Fraga Filho, Rio de Janeiro (RJ) entre 2001 e 2006. MÉTODOS: Durante o período do estudo, 34 isolados de MNT de sítios estéreis de 14 pacientes, a maioria HIV positivos, foram submetidos a identificação fenotípica e hsp65 PCR-restriction enzyme analysis (PRA, análise por enzimas de restrição por PCR do gene hsp65). RESULTADOS: A maioria dos isolados foi identificada como Mycobacterium avium, seguida por M. monacense, M. kansasii e M. abscessus em menores proporções. CONCLUSÕES: A combinação de PRA, um método relativamente simples e de baixo custo, com algumas características fenotípicas pode fornecer a identificação correta de MNT na rotina de laboratórios clínicos.

scholarly journals PCR-Restriction Enzyme Analysis of a Bone Marrow Isolate from a Human Immunodeficiency Virus-Positive Patient Discloses Polyclonal Infection with Two Mycobacterium avium Strains

2000 ◽  
Vol 38 (12) ◽  
pp. 4643-4645 ◽  
Author(s):  
R. S. Oliveira ◽  
M. P. Sircili ◽  
S. Y. M. Ueki ◽  
M. A. S. Telles ◽  
B. Schnabel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mycobacterium Avium ◽  
Restriction Enzyme ◽  
Fragment Length Polymorphism ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Insertion Element ◽  
Immunodeficiency Virus ◽  
Digestion Pattern ◽  
Polyclonal Infection

Polyclonal infection by Mycobacterium avium was detected by hsp65 PCR-restriction enzyme analysis (PRA) in a bone marrow isolate from an AIDS patient. Two M. aviumstrains, differing in colony morphology, PRA HaeIII digestion pattern, insertion element (IS) 1245amplification, and restriction fragment length polymorphism fingerprints with IS1245 and IS1311 probes, were isolated.

scholarly journals Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR-Restriction Enzyme Analysis

1999 ◽  
Vol 37 (8) ◽  
pp. 2592-2597 ◽  
Author(s):  
Sylvia Cardoso Leão ◽  
Marcelo R. S. Briones ◽  
Marcelo Palma Sircili ◽  
Simone Carvalho Balian ◽  
Nelson Mores ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Mycobacterium Avium ◽  
Restriction Enzyme ◽  
Clinical Laboratory ◽  
Epidemiological Studies ◽  
Nucleic Acid Hybridization ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Biochemical Tests ◽  
Diagnostic Significance

Mycobacterium avium complex (MAC) is composed of environmental mycobacteria found widely in soil, water, and aerosols that can cause disease in animals and humans, especially disseminated infections in AIDS patients. MAC consists of two closely related species, M. avium and M. intracellulare, and may also include other, less-defined groups. The precise differentiation of MAC species is a fundamental step in epidemiological studies and for the evaluation of possible reservoirs for MAC infection in humans and animals. In this study, which included 111 pig and 26 clinical MAC isolates, two novel allelic M. aviumPCR-restriction enzyme analysis (PRA) variants were identified, differing from the M. avium PRA prototype in theHaeIII digestion pattern. Mutations in HaeIII sites were confirmed by DNA sequencing. Identification of these isolates as M. avium was confirmed by PCR with DT1-DT6 and IS1245 primers, nucleic acid hybridization with the AccuProbe system, 16S ribosomal DNA sequencing, and biochemical tests. The characterization of M. avium PRA variants can be useful in the elucidation of factors involved in mycobacterial virulence and routes of infection and also has diagnostic significance, since they can be misidentified as M. simiae II and M. kansasii I if the PRA method is used in the clinical laboratory for identification of mycobacteria.

scholarly journals Isolamento de micobactérias não-tuberculosas em São José do Rio Preto entre 1996 e 2005

2008 ◽  
Vol 34 (11) ◽  
pp. 950-955 ◽  
Author(s):  
Heloisa da Silveira Paro Pedro ◽  
Maria Izabel Ferreira Pereira ◽  
Maria do Rosário Assad Goloni ◽  
Suely Yoko Mizuka Ueki ◽  
Erica Chimara
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Avium ◽  
Restriction Enzyme ◽  
American Thoracic Society ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

OBJETIVO: Estudar a ocorrência de micobactérias não-tuberculosas e a variabilidade das espécies isoladas na região atendida pelo Instituto Adolfo Lutz-Regional de São José do Rio Preto-no período entre 1996 e 2005, assim como mostrar a importância do diagnóstico laboratorial. MÉTODOS: A partir de amostras pulmonares e extrapulmonares, foi realizado o isolamento de micobactérias, e estas foram identificadas por métodos fenotípicos e pelo método molecular polymerase chain reaction-restriction enzyme analysis. RESULTADOS: Foram isoladas 317 cepas de micobactérias não-tuberculosas: complexo Mycobacterium avium, 182 (57,4%); M. gordonae, 33 (10,4%); M. fortuitum, 25(7,9%); M. chelonae, 8 (2,5%); complexo M. terrae, 8 (2,5%); M. kansasii, 7 (2,2%); e espécies menos freqüentes, 54 (17%). No período, foram caracterizados 72 casos (33,3%) de micobacterioses, de acordo com os critérios bacteriológicos estabelecidos pela American Thoracic Society (2007).Desses, complexo M. avium foi responsável por 56 casos, sendo que 29 (51,8%) foram caracterizados como doença disseminada. M. fortuitum foi responsável por 6 casos; M. gordonae, 3; M. chelonae, 2; M. abscessus, 1; M. kansasii, 1; M. intracellulare, 1; M. malmoense, 1; e Mycobacterium ssp., 1. CONCLUSÕES: Os resultados obtidos mostraram a importância do diagnóstico bacteriológico das micobacterioses, pois a identificação das espécies possibilita a introdução de um tratamento adequado precocemente.

Long interspersed repeated DNA (LINE) causes polymorphism at the rat insulin 1 locus

1985 ◽  
Vol 5 (9) ◽  
pp. 2197-2203
Author(s):  
M S Lakshmikumaran ◽  
E D'Ambrosio ◽  
L A Laimins ◽  
D T Lin ◽  
A V Furano
Keyword(s):  
Dna Sequence ◽  
Restriction Enzyme ◽  
Allelic Variation ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Repeated Dna ◽  
Coding Region ◽  
Sequence Determination ◽  
Polymorphic Region ◽  
Or Gene

The insulin 1, but not the insulin 2, locus is polymorphic (i.e., exhibits allelic variation) in rats. Restriction enzyme analysis and hybridization studies showed that the polymorphic region is 2.2 kilobases upstream of the insulin 1 coding region and is due to the presence or absence of an approximately 2.7-kilobase repeated DNA element. DNA sequence determination showed that this DNA element is a member of a long interspersed repeated DNA family (LINE) that is highly repeated (greater than 50,000 copies) and highly transcribed in the rat. Although the presence or absence of LINE sequences at the insulin 1 locus occurs in both the homozygous and heterozygous states, LINE-containing insulin 1 alleles are more prevalent in the rat population than are alleles without LINEs. Restriction enzyme analysis of the LINE-containing alleles indicated that at least two versions of the LINE sequence may be present at the insulin 1 locus in different rats. Either repeated transposition of LINE sequences or gene conversion between the resident insulin 1 LINE and other sequences in the genome are possible explanations for this.

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