scholarly journals Use of the BacT/Alert Blood Culture System for Culture of Sterile Body Fluids Other than Blood

1998 ◽  
Vol 36 (11) ◽  
pp. 3273-3277 ◽  
Author(s):  
Paul Bourbeau ◽  
Julie Riley ◽  
Barbara J. Heiter ◽  
Ron Master ◽  
Carol Young ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Body Fluids ◽  
Coagulase Negative Staphylococci ◽  
Culture Methods ◽  
Gram Positive ◽  
Blood Culture System ◽  
Anaerobic Bottle ◽  
Gram Positive Cocci ◽  
Sterile Body Fluids

Studies have demonstrated that large-volume culture methods for sterile body fluids other than blood increase recovery compared to traditional plated-medium methods. BacT/Alert is a fully automated blood culture system for detecting bacteremia and fungemia. In this study, we compared culture in BacT/Alert standard aerobic and anaerobic bottles, BacT/Alert FAN aerobic and FAN anaerobic bottles, and culture on routine media for six specimen types, i.e., continuous ambulatory peritoneal dialysate (CAPD), peritoneal, amniotic, pericardial, synovial, and pleural fluids. Specimen volumes were divided equally among the three arms of the study. A total of 1,157 specimens were tested, with 227 significant isolates recovered from 193 specimens. Recovery by method was as follows: standard bottles, 186 of 227 (82%); FAN bottles, 217 of 227 (96%); and routine culture, 184 of 227 (81%). The FAN bottles recovered significantly more gram-positive cocci (P < 0.001), Staphylococcus aureus(P = 0.003), coagulase-negative staphylococci (P = 0.008), gram-negative bacilli (P< 0.001), Enterobacteriaceae (P = 0.005), and total organisms (P < 0.001) than the routine culture. There were no significant differences in recovery between the standard bottles and the routine culture. The FAN aerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), coagulase-negative staphyococci (P = 0.003), and total organisms (P< 0.001) than the standard aerobic bottle, while the FAN anaerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), Enterobacteriaceae(P = 0.03), and total organisms (P < 0.001) than the standard anaerobic bottle. For specific specimen types, significantly more isolates were recovered from the FAN bottles compared to the routine culture for synovial (P < 0.001) and CAPD (P = 0.004) fluids. Overall, the FAN bottles were superior in performance to both the standard bottles and the routine culture for detection of microorganisms from the types of sterile body fluids included in this study.

Comparison of the BACTEC blood culture system versus conventional methods for culture of normally sterile body fluids

2007 ◽  
Vol 24 (6) ◽  
pp. 1271-1277 ◽  
Author(s):  
Emel Sesli Çetin ◽  
Selçuk Kaya ◽  
Mustafa Demirci ◽  
Buket Cicioglu Aridogan
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Body Fluids ◽  
Blood Culture System ◽  
System Versus ◽  
Conventional Methods ◽  
Sterile Body Fluids

scholarly journals Evaluation of Four Methods for Rapid Identification of Staphylococcus aureus from Blood Cultures

1998 ◽  
Vol 36 (4) ◽  
pp. 1032-1034 ◽  
Author(s):  
David J. Speers ◽  
Thomas R. Olma ◽  
Gwendolyn L. Gilbert
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Culture System ◽  
Rapid Identification ◽  
Culture Broth ◽  
Blood Cultures ◽  
Blood Culture System ◽  
Variable Sensitivity ◽  
Rapid Tests ◽  
Gram Positive Cocci

The identification of Staphylococcus aureus directly from blood cultures is clinically relevant, but it requires a test that is both rapid and reliable. Previously, biochemical, immunological, tube coagulase, and thermostable-endonuclease methods have shown variable sensitivity and specificity. Testing directly from blood culture broth has not been described for the latex kit Staphaurex Plus (Murex Diagnostics Ltd.), and the modified conventional tests have not been used with the newer, continuously monitored blood culture systems. In addition, the commercial RAPIDEC staph kit (bioMerieux Vitek, Inc.) has been used to detect S. aureus directly from the Vital blood culture system (bioMerieux, Marcy l’Etoile, France), but its performance has not been evaluated with other continuously monitored systems. A total of 201 clinical blood cultures (BACTEC 9240 culture system; Johnston Laboratories, Inc.) in which a Gram stain showed gram-positive cocci resembling staphylococci were evaluated prospectively. The Staphaurex Plus kit, the tube coagulase test, the thermostable-endonuclease test, and the RAPIDEC staph kit were compared. The sensitivities were 23, 92, 85, and 98% and the specificities were 99, 100, 93, and 100%, respectively. The RAPIDEC staph kit was the most reliable test, with a diagnostic accuracy comparable to that of the best published results for any of the rapid tests. However, it was the most expensive of the tests and relatively labor-intensive. The tube coagulase test was also sensitive, the simplest to perform, and inexpensive.

scholarly journals Evaluation of Blood Culture System for Culture of Body Fluids

2015 ◽  
Vol 37 (1) ◽  
pp. 23-28
Author(s):  
Soon Deok Park ◽  
Young Uh ◽  
In Ho Jang ◽  
Maria Hong ◽  
Hyeun Gyeo Lee ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Body Fluids ◽  
Blood Culture System

scholarly journals Blood culture technique based on centrifugation: developmental phase

1976 ◽  
Vol 3 (3) ◽  
pp. 251-257
Author(s):  
G L Dorn ◽  
J R Haynes ◽  
G G Burson
Keyword(s):  
Escherichia Coli ◽  
Blood Culture ◽  
Culture System ◽  
Culture Technique ◽  
Developmental Phase ◽  
Culture Methods ◽  
Blood Culture System ◽  
Relative Centrifugal Force ◽  
Normal Human ◽  
Normal Human Blood

A quantitative and flexible blood culture system based on centrifugation of lysed blood over a density layer is described. The effect of relative centrifugal force, centrifugation time, and two different density solutions on the recovery of Staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, and Candida albicans was determined. To demonstrate the versatility of this technique, the reisolation of 23 representative microorganisms from inoculated normal human blood was determined at a fixed centrifugation speed and time. The potential merits of this technique are discussed and compared with those of conventional blood culture methods.

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