Utility of Bact/Alert Blood Culture System for Culture of Sterile Body Fluids in a Tertiary Care Hospital

Background: Blood Culture is the gold standard and accurate method of diagnosing bacteremia in enteric fever; however, conventional blood culture is slow in isolating Salmonella typhi and Salmonella paratyphi.Objective: The main aim of this study was to compare the result of the lytic centrifugation method with conventional blood culture system for the accurate diagnosis of enteric fever in febrile patients.Methodology: The cross-sectional study was carried out in the department of Microbiology, Mymensingh Medical College, Mymensingh between July 2010 and June 2011 including 200 individuals of different ages and sexes. Of them, 150 were clinically suspected cases of typhoid fever and 50 controls comprising of 25 non-typhoidal febrile patients and 25 healthy individuals. Both types of blood culture were performed for each of the cases and controls.Result: The lytic centrifugation method recovered more organisms (17.3%) than the conventional blood culture method (13.3%). Time required for isolation of S. typhi and S. paratyphi A was short in lytic method (18-20hours) than conventional method (42-72hours). Total contamination rate was 0.5% by lytic as compared to the conventional blood culture method which was 5.0%.Conclusion: In conclusion the lytic method is better than conventional blood culture system for good result, short isolation time and less chance of contamination.Bangladesh Journal of Infectious Diseases 2016;3(1):6-10

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Comparative evaluation of the role of single and multiple blood specimens in the outcome of blood cultures using BacT/ALERT 3D (automated) blood culture system in a tertiary care hospital

Indian Journal of Critical Care Medicine ◽

10.5005/ijccm-20-9-530 ◽

2016 ◽

Vol 20 (9) ◽

pp. 530-533 ◽

Cited By ~ 3

Author(s):

Prithwis Bhattacharyya ◽

Himesh Barman ◽

D. Elantamilan ◽

Valarie Lyngdoh ◽

Annie B. Khyriem ◽

...

Keyword(s):

Comparative Evaluation ◽

Culture System ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Blood Cultures ◽

Care Hospital ◽

Blood Culture System ◽

Multiple Blood ◽

Blood Specimens

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Aerobic Bacteriological Profile and Antimicrobial Sensitivity Pattern of Bacteria Isolated from Sterile Body Fluids: A Study from a Tertiary Care Hospital in North India

Microbiology Research Journal International ◽

10.9734/mrji/2019/v28i130123 ◽

2019 ◽

pp. 1-10

Author(s):

Mariya Rouf ◽

Asifa Nazir

Keyword(s):

Synovial Fluid ◽

Pleural Fluid ◽

Ascitic Fluid ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Body Fluids ◽

Pericardial Fluid ◽

Care Hospital ◽

E Coli ◽

Sterile Body Fluids

Aims: This study aims to isolate and identify the aerobic bacterial pathogens of sterile body fluids and to determine their susceptibility to various antibacterial agents. Study Design: This study was a retrospective observational study conducted in a tertiary care hospital. Place and Duration of Study: This study was conducted in the Department of Microbiology, SMHS hospital, Srinagar. A total of 814 samples were analysed for bacteriological culture and antibiotic sensitivity over a period of one year, from April 2018 to March 2019. Methodology: Clinical specimens (pleural fluid, ascitic fluid, cerebrospinal fluid, Synovial fluid, pericardial fluid and bile) were processed for bacterial culture according to standard procedures and antimicrobial susceptibility test for isolated organisms was done using Kirby Bauer disc diffusion method and interpreted as per Clinical and Laboratory Standards Institute (CLSI) recommendations. Results: In 814 samples of various body fluids, 88 samples showed growth of organism with an isolation rate of 10.81%. growth was most commonly seen in CSF (34.09%) followed by Ascitic fluid (23.86%, Bile (20.45), Pleural fluid (15.90%) and Synovial fluid (5.68%). No growth was obtained from pericardial fluid. The most predominant isolates were E. coli (23.86%), Pseudomonas sp (15.90%), Acinetobacter (14.77%), Klebsiella sp (7.95%), Staphylococcus aureus (11.36%), CONS (12.5%) and Enterococcus sp (4.54%). E. coli and Klebsiella were sensitive to imipenem, meropenem. colistin, amikacin and gentamicin. Staph. aureus and CONS were mostly sensitive to vancomycin, linezolid, and teicoplanin. Pseudomonas was sensitive to imipenem, meropenem, colistin and piperacillin/tazobactam. Acinetobacter, E. coli and Klebsiella sp were the most resistant organisms. Conclusion: In our study significant numbers of multidrug resistant bacteria were isolated from body fluids which calls for regular monitoring of prevalent pathogenic organisms and their sensitivities to avoid indiscriminate use of unnecessary antibiotics and the development of antibiotic resistance.

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Evaluation of BACTEC™ blood culture system for culture of normally sterile body fluids

Indian Journal of Critical Care Medicine ◽

10.4103/0972-5229.146331 ◽

2014 ◽

Vol 18 (12) ◽

pp. 829-830 ◽

Cited By ~ 2

Author(s):

Uttam Udayan ◽

Meena Dias

Keyword(s):

Blood Culture ◽

Culture System ◽

Body Fluids ◽

Blood Culture System ◽

Sterile Body Fluids

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650. Clinical Performance Evaluation of Virtuo Blood Culture System in a Tertiary Care Hospital

Open Forum Infectious Diseases ◽

10.1093/ofid/ofaa439.844 ◽

2020 ◽

Vol 7 (Supplement_1) ◽

pp. S383-S383

Author(s):

Miguel A Chavez ◽

Satish Munigala ◽

Carey-Ann Burnham ◽

Melanie L Yarbrough ◽

David K Warren

Keyword(s):

Blood Culture ◽

Recovery Rate ◽

Culture System ◽

Tertiary Care ◽

Blood Cultures ◽

Care Hospital ◽

Blood Culture System ◽

Culture Positivity ◽

Blood Culture Positivity ◽

Staphylococcus Spp

Abstract Background Bloodstream infections are a major cause of morbidity and mortality. BACT/ALERT VIRTUO (VIRTUO) blood culture system is an automated, closed system used with resin-containing media which may enhance the growth of microorganisms. Our objective was to assess the real-world performance of the VIRTUO system. Methods We retrospectively reviewed all blood cultures performed between January-December 2018 (VersaTREK) and January-December 2019 (VIRTUO) at a 1250-bed academic medical center. Blood culture positivity rates, contamination rates, and time from collection to arrival in the laboratory were compared pre- versus post-VIRTUO implementation. Contamination was defined as a single blood culture with common skin microbiota. Results A total of 101803 blood cultures were performed during the study period: 48969 (48.1%) were processed with VersaTREK system and 52834 (51.9%) with VIRTUO system. A decreased median time from collection until arrival to the laboratory was seen post-implementation (2.0 pre- vs. 0.8 hours post-implementation, p< 0.001). The positivity rate increased from 3987 (8.1%) pre-implementation to 6141 (11.6%) post-implementation (p < 0.001) (Table and Figure). Staphylococcus aureus was the most frequently isolated species for both periods and had higher recovery rate with the VIRTUO system (717 (1.5%) pre- vs. 1764 (3.3%) post-implementation, p< 0.001). Higher recovery rate was also noted for other Staphylococcus spp. in the post-implementation period (985 (2.0%) pre- vs. 1644 (3.1%) post-implementation, p< 0.001). No difference in the organism recovery rate was noted for Streptococcus spp., Enterococcus faecium, E. faecalis, Pseudomonas aeruginosa, Enterobacterales, and Candida spp. The inpatient contamination rate was higher post-implementation (1.5% pre- vs. 1.9% post-implementation, p < 0.001). Comparison of blood culture positivity rate pre- vs. post-implementation, by culture location Daily positivity rate for blood cultures processed at BJH during the study period Conclusion The VIRTUO system showed a higher rate of positive blood cultures compared to the VersaTREK system primarily from a higher detection of Staphylococcus spp. Further studies are needed to assess whether an increased rate of positive blood cultures is associated with changes in management and clinical outcomes. Disclosures All Authors: No reported disclosures

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Use of the BacT/Alert Blood Culture System for Culture of Sterile Body Fluids Other than Blood

Journal of Clinical Microbiology ◽

10.1128/jcm.36.11.3273-3277.1998 ◽

1998 ◽

Vol 36 (11) ◽

pp. 3273-3277 ◽

Cited By ~ 40

Author(s):

Paul Bourbeau ◽

Julie Riley ◽

Barbara J. Heiter ◽

Ron Master ◽

Carol Young ◽

...

Keyword(s):

Blood Culture ◽

Culture System ◽

Body Fluids ◽

Coagulase Negative Staphylococci ◽

Culture Methods ◽

Gram Positive ◽

Blood Culture System ◽

Anaerobic Bottle ◽

Gram Positive Cocci ◽

Sterile Body Fluids

Studies have demonstrated that large-volume culture methods for sterile body fluids other than blood increase recovery compared to traditional plated-medium methods. BacT/Alert is a fully automated blood culture system for detecting bacteremia and fungemia. In this study, we compared culture in BacT/Alert standard aerobic and anaerobic bottles, BacT/Alert FAN aerobic and FAN anaerobic bottles, and culture on routine media for six specimen types, i.e., continuous ambulatory peritoneal dialysate (CAPD), peritoneal, amniotic, pericardial, synovial, and pleural fluids. Specimen volumes were divided equally among the three arms of the study. A total of 1,157 specimens were tested, with 227 significant isolates recovered from 193 specimens. Recovery by method was as follows: standard bottles, 186 of 227 (82%); FAN bottles, 217 of 227 (96%); and routine culture, 184 of 227 (81%). The FAN bottles recovered significantly more gram-positive cocci (P < 0.001), Staphylococcus aureus(P = 0.003), coagulase-negative staphylococci (P = 0.008), gram-negative bacilli (P< 0.001), Enterobacteriaceae (P = 0.005), and total organisms (P < 0.001) than the routine culture. There were no significant differences in recovery between the standard bottles and the routine culture. The FAN aerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), coagulase-negative staphyococci (P = 0.003), and total organisms (P< 0.001) than the standard aerobic bottle, while the FAN anaerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), Enterobacteriaceae(P = 0.03), and total organisms (P < 0.001) than the standard anaerobic bottle. For specific specimen types, significantly more isolates were recovered from the FAN bottles compared to the routine culture for synovial (P < 0.001) and CAPD (P = 0.004) fluids. Overall, the FAN bottles were superior in performance to both the standard bottles and the routine culture for detection of microorganisms from the types of sterile body fluids included in this study.

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