scholarly journals Molecular Characterization of a Shiga ToxigenicEscherichia coli O113:H21 Strain Lacking eaeResponsible for a Cluster of Cases of Hemolytic-Uremic Syndrome

1999 ◽  
Vol 37 (10) ◽  
pp. 3357-3361 ◽  
Author(s):  
Adrienne W. Paton ◽  
Matthew C. Woodrow ◽  
Robyn M. Doyle ◽  
Janice A. Lanser ◽  
James C. Paton
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Molecular Characterization ◽  
Gastrointestinal Disease ◽  
Diverse Group ◽  
Uremic Syndrome

Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, certain strains appear to have greater virulence for humans. STEC strains carryingeae and belonging to serogroup O157 or O111 have been responsible for the vast majority of outbreaks of STEC disease reported to date. Here we describe a STEC O113:H21 strain lackingeae that was responsible for a cluster of three cases of hemolytic-uremic syndrome. This strain produces a single Stx2-related toxin and adheres efficiently to Henle 407 cells.

scholarly journals Detection and Characterization of Shiga ToxigenicEscherichia coli by Using Multiplex PCR Assays forstx 1, stx 2,eaeA, Enterohemorrhagic E. coli hlyA,rfb O111, andrfb O157

1998 ◽  
Vol 36 (2) ◽  
pp. 598-602 ◽  
Author(s):  
Adrienne W. Paton ◽  
James C. Paton
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Genetic Characterization ◽  
Gastrointestinal Disease ◽  
Diverse Group ◽  
E Coli ◽  
Pcr Products ◽  
Uremic Syndrome ◽  
Pcr Assays

Shiga toxigenic Escherichia coli (STEC) comprises a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, certain strains appear to be of greater virulence for humans, for example, those belonging to serogroups O111 and O157 and those with particular combinations of other putative virulence traits. We have developed two multiplex PCR assays for the detection and genetic characterization of STEC in cultures of feces or foodstuffs. Assay 1 utilizes four PCR primer pairs and detects the presence of stx 1,stx 2 (including variants ofstx 2), eaeA, and enterohemorrhagicE. coli hlyA, generating amplification products of 180, 255, 384, and 534 bp, respectively. Assay 2 uses two primer pairs specific for portions of the rfb (O-antigen-encoding) regions of E. coli serotypes O157 and O111, generating PCR products of 259 and 406 bp, respectively. The two assays were validated by testing 52 previously characterized STEC strains and observing 100% agreement with previous results. Moreover, assay 2 did not give a false-positive O157 reaction with enteropathogenic E. colistrains belonging to clonally related serogroup O55. Assays 1 and 2 detected STEC of the appropriate genotype in primary fecal cultures from five patients with hemolytic-uremic syndrome and three with bloody diarrhea. Thirty-one other primary fecal cultures from patients without evidence of STEC infection were negative.

scholarly journals Whole-genome characterization of hemolytic uremic syndrome-causing Shiga toxin-producing Escherichia coli in Sweden

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1296-1305
Author(s):  
Ying Hua ◽  
Milan Chromek ◽  
Anne Frykman ◽  
Cecilia Jernberg ◽  
Valya Georgieva ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Shiga Toxin ◽  
Whole Genome ◽  
Genome Characterization ◽  
Uremic Syndrome

scholarly journals Genotypic Characterization of Non-O157 Shiga Toxin–Producing Escherichia coli in Beef Abattoirs of Argentina

2011 ◽  
Vol 74 (12) ◽  
pp. 2008-2017 ◽  
Author(s):  
M. O. MASANA ◽  
B. A. D'ASTEK ◽  
P. M. PALLADINO ◽  
L. GALLI ◽  
L. L. DEL CASTILLO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Shiga Toxin ◽  
Pattern Analysis ◽  
Severe Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Lower Proportion ◽  
Uremic Syndrome ◽  
Sporadic Cases

The non-O157 Shiga toxin–producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with non-O157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease.

Genotypic characterization of Escherichia coli O157:H7 strains that cause diarrhea and hemolytic uremic syndrome in Neuquén, Argentina

2014 ◽  
Vol 304 (3-4) ◽  
pp. 499-504 ◽  
Author(s):  
Pianciola Luis ◽  
Chinen Isabel ◽  
Mazzeo Melina ◽  
Miliwebsky Elizabeth ◽  
González Gladys ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Escherichia Coli O157 ◽  
Genotypic Characterization ◽  
Uremic Syndrome

scholarly journals Characterization of a Novel Type IV Pilus Locus Encoded on the Large Plasmid of Locus of Enterocyte Effacement-Negative Shiga-Toxigenic Escherichia coli Strains That Are Virulent for Humans

2002 ◽  
Vol 70 (6) ◽  
pp. 3094-3100 ◽  
Author(s):  
Potjanee Srimanote ◽  
Adrienne W. Paton ◽  
James C. Paton
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Disease ◽  
Large Plasmid ◽  
Type Iv Pilus ◽  
Upstream Gene ◽  
Type Iv ◽  
Uremic Syndrome ◽  
Enterocyte Effacement

ABSTRACT The majority of Shiga-toxigenic Escherichia coli (STEC) strains isolated from humans with gastrointestinal disease carry large (approximately 90-kb) plasmids. We have been analyzing the megaplasmid (designated pO113) from an O113:H21 STEC strain (98NK2). This strain lacks the locus for enterocyte effacement (LEE) and yet was responsible for an outbreak of hemolytic uremic syndrome. In the present study, we demonstrate that pO113 carries a novel type IV pilus biosynthesis locus (pil) related to those of the IncI plasmids R721, R64, and ColIb9. The pO113 pil locus consists of 11 closely linked genes (pilL through pilV) with an additional separately transcribed upstream gene (pilI). It directs the expression of long thin pili on the 98NK2 surface and the hemagglutination of guinea pig erythrocytes. We also demonstrate that pO113 can be transferred by conjugation. However, the type IV pilus encoded by pO113 does not appear to be involved in the adherence of 98NK2 to HEp-2 or Hct-8 cells in vitro. Homologues of the pO113 pil locus were present in several other LEE-negative STEC strains but not in LEE-positive STEC strains belonging to serogroup O26, O111, or O157.

scholarly journals Molecular Characterization of Human Atypical Sorbitol-Fermenting Enteropathogenic Escherichia coli O157 Reveals High Diversity

2016 ◽  
Vol 54 (5) ◽  
pp. 1357-1363 ◽  
Author(s):  
Annelene Kossow ◽  
Wenlan Zhang ◽  
Martina Bielaszewska ◽  
Sophie Rhode ◽  
Kevin Hansen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Symptoms ◽  
Diverse Group ◽  
Full Spectrum ◽  
Content Type ◽  
E Coli ◽  
Uremic Syndrome ◽  
Encoding Gene ◽  
Encoding Genes

Alongside the well-characterized enterohemorrhagicEscherichia coli(EHEC) O157:H7, serogroup O157 comprises sorbitol-fermenting typical and atypical enteropathogenicE. coli(EPEC/aEPEC) strains that carry the intimin-encoding geneeaebut not Shiga toxin-encoding genes (stx). Since little is known about these pathogens, we characterized 30 clinical isolates from patients with hemolytic uremic syndrome (HUS) or uncomplicated diarrhea with respect to their flagellin gene (fliC) type and multilocus sequence type (MLST). Moreover, we applied whole-genome sequencing (WGS) to determine the phylogenetic relationship with othereae-positive EHEC serotypes and the composition of therfbO157 region.fliCtyping resulted in fivefliCtypes (H7, H16, H34, H39, and H45). Isolates of eachfliCtype shared a unique ST. In comparison to the 42 HUS-associatedE. coli(HUSEC) strains, only thestx-negative isolates withfliCH7 shared their ST with EHEC O157:H7/H−strains. With the exception of one O157:H−fliCH16isolate, HUS was exclusively associated withfliCH7. WGS corroborated the separation of thefliCH7 isolates, which were closely related to the EHEC O157:H7/H−isolates, and the diverse group of isolates exhibiting differentfliCtypes, indicating independent evolution of the different serotypes. This was also supported by the heterogeneity within therfbO157 region that exhibited extensive recombinations. The genotypic subtypes and distribution of clinical symptoms suggested that thestx-negative O157 strains withfliCH7 were originally EHEC strains that loststx. The remaining isolates form a distinct and diverse group of atypical EPEC isolates that do not possess the full spectrum of virulence genes, underlining the importance of identifying the H antigen for clinical risk assessment.

scholarly journals Risk of Hemolytic Uremic Syndrome Related to Treatment of Escherichia coli O157 Infection with Different Antimicrobial Classes

2021 ◽  
Vol 9 (9) ◽  
pp. 1997
Author(s):  
Rajal K. Mody ◽  
Robert M. Hoekstra ◽  
Magdalena Kendall Scott ◽  
John Dunn ◽  
Kirk Smith ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Medical Records ◽  
Antimicrobial Treatment ◽  
Escherichia Coli O157 ◽  
Full Characterization ◽  
Patient Interviews ◽  
Uremic Syndrome ◽  
Control Study

Treatment of Shiga toxin-producing Escherichia coli O157 (O157) diarrhea with antimicrobials might alter the risk of hemolytic uremic syndrome (HUS). However, full characterization of which antimicrobials might affect risk is lacking, particularly among adults. To inform clinical management, we conducted a case-control study of residents of the FoodNet surveillance areas with O157 diarrhea during a 4-year period to assess antimicrobial class-specific associations with HUS among persons with O157 diarrhea. We collected data from medical records and patient interviews. We measured associations between treatment with agents in specific antimicrobial classes during the first week of diarrhea and development of HUS, adjusting for age and illness severity. We enrolled 1308 patients; 102 (7.8%) developed confirmed HUS. Antimicrobial treatment varied by age: <5 years (12.6%), 5–14 (11.5%), 15–39 (45.4%), ≥40 (53.4%). Persons treated with a β-lactam had higher odds of developing HUS (OR 2.80, CI 1.14–6.89). None of the few persons treated with a macrolide developed HUS, but the protective association was not statistically significant. Exposure to “any antimicrobial” was not associated with increased odds of HUS. Our findings confirm the risk of β-lactams among children with O157 diarrhea and extends it to adults. We observed a high frequency of inappropriate antimicrobial treatment among adults. Our data suggest that antimicrobial classes differ in the magnitude of risk for persons with O157 diarrhea.

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