scholarly journals Analysis of Plasmid and Chromosomal DNA of Multidrug-Resistant Salmonella enterica Serovar Typhi from Asia

2000 ◽  
Vol 38 (4) ◽  
pp. 1449-1452 ◽  
Author(s):  
S. Mirza ◽  
S. Kariuki ◽  
K. Z. Mamun ◽  
N. J. Beeching ◽  
C. A. Hart
Keyword(s):  
Restriction Endonuclease ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Restriction Endonuclease Analysis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Chromosomal Dna ◽  
Incompatibility Group ◽  
Salmonella Enterica Serovar Typhi ◽  
Endonuclease Analysis

Molecular analysis of chromosomal DNA from 193 multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates from 1990 to 1995 from Pakistan, Kuwait, Malaysia, Bangladesh, and India produced a total of five major different pulsed-field gel electrophoresis (PFGE) patterns. Even within a particular country MDR S. entericaserovar Typhi DNA was found to be in different PFGE groups. Similar self-transferable 98-MDa plasmids belonging to either incompatibility group incHI1 or incHI1/FIIA were implicated in the MDR phenotype inS. enterica serovar Typhi isolates from all the locations except Quetta, Pakistan, where the majority were of incFIA. A total of five different PFGE genotypes with six different plasmids, based on incompatibility and restriction endonuclease analysis groups, were found among these MDR S. enterica serovar Typhi isolates.

Monitoring lactic acid bacteria strains during ‘Cacioricotta’ cheese production by restriction endonuclease analysis and pulsed-field gel electrophoresis

2001 ◽  
Vol 68 (1) ◽  
pp. 139-144 ◽  
Author(s):  
GIUSEPPE BLAIOTTA ◽  
GIANCARLO MOSCHETTI ◽  
ERMENEGILDA SIMEOLI ◽  
ROSAMARIA ANDOLFI ◽  
FRANCESCO VILLANI ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Restriction Endonuclease ◽  
Gel Electrophoresis ◽  
Water Buffalo ◽  
Restriction Endonuclease Analysis ◽  
Buffalo Milk ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Endonuclease Analysis

Lactic acid bacteria (LAB) play an important role in food fermentation, as the products obtained with their aid are characterized by hygienic safety, storage stability and attractive sensory properties. A major aim of the research in this field is the selection of LAB strains that could be used. Hence it is very important to be able to apply a reliable method to distinguish a particular strain specifically and unambiguously, which allows studies of population dynamics of mixed cultures and monitoring starter strains during fermentation (Ramos & Harlander, 1990).Molecular methods are a powerful alternative to the traditional differentiation of bacteria. A highly reproducible method for characterizing and distinguishing closely related strains, is represented by REA-PFGE (restriction endonuclease analysis by pulsed-field gel electrophoresis) performed by infrequently cutting endonucleases. Genetic differentiation of strains in several species of LAB has been successfully performed by this technique (Moschetti et al. 1997; Villani et al. 1997) obtaining very clear and reproducible restriction patterns (Moschetti et al. 1998).In this study selected inoculated strains (lactococci or lactobacilli) were monitored by REA-PFGE during the whole process of water-buffalo ‘cacioricotta’ cheese-making. This product is a typical and traditional cheese in southern Italy produced from cow, goat, ewe or water-buffalo milk. The original technology of this preparation permits the recovery of whey proteins due to the high heat treatment of whole milk employed, allowing interesting yields to be achieved in terms of cheese. The use of starters is not common in traditional technology but low acid protection of the final product suggested the use of LAB as starter (Emaldi et al. 1987).

scholarly journals Typing of Salmonella enterica Serotype Paratyphi C Isolates from Various Countries by Plasmid Profiles and Pulsed-Field Gel Electrophoresis

1999 ◽  
Vol 37 (6) ◽  
pp. 2058-2060 ◽  
Author(s):  
S. Kariuki ◽  
J. Cheesbrough ◽  
A. K. Mavridis ◽  
C. A. Hart
Keyword(s):  
Restriction Endonuclease ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Plasmid Profiles ◽  
Similar Restriction ◽  
Restriction Endonuclease Digest ◽  
Pfge Profiles

Pulsed-field gel electrophoresis (PFGE) of 61 Salmonella enterica serotype Paratyphi C isolates from six countries gave five distinct clusters. Twenty-four isolates from five countries were susceptible to 10 antimicrobials tested and gave similar restriction endonuclease digest patterns of the 38-MDa plasmid. In contrast, plasmid and PFGE profiles of 37 multidrug-resistant isolates from Zaire were different from those from other countries.

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