scholarly journals Bartonella vinsonii subsp.berkhoffii as an Agent of Afebrile Blood Culture-Negative Endocarditis in a Human

2000 ◽  
Vol 38 (4) ◽  
pp. 1698-1700 ◽  
Author(s):  
Véronique Roux ◽  
Susannah J. Eykyn ◽  
Sarah Wyllie ◽  
Didier Raoult
Keyword(s):  
Blood Culture ◽  
Ribosomal Dna ◽  
Citrate Synthase ◽  
Pcr Amplification ◽  
Intergenic Spacer ◽  
Bacterial Identification ◽  
Gene Encoding ◽  
Intergenic Spacer Region ◽  
16S Ribosomal Dna ◽  
Culture Negative

We report a case of endocarditis in a human infected withBartonella vinsonii subsp. berkhoffii, which causes bacteremia and endocarditis in dogs. Bacterial identification was established by PCR amplification and sequencing of an intergenic spacer region (ITS1), 16S ribosomal DNA, and a gene encoding citrate synthase (gltA). Bartonella antibodies were detected by immunofluorescence.

scholarly journals PCR amplification and polymorphism analysis of the intergenic spacer region of ribosomal DNA in Tuber borchii

2002 ◽  
Vol 157 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Pasquapina Ciarmela ◽  
Lucia Potenza ◽  
Luigi Cucchiarini ◽  
Sabrina Zeppa ◽  
Vilberto Stocchi
Keyword(s):  
Ribosomal Dna ◽  
Pcr Amplification ◽  
Intergenic Spacer ◽  
Polymorphism Analysis ◽  
Tuber Borchii ◽  
Intergenic Spacer Region

16S-ribosomal DNA to diagnose culture-negative endocarditis

2008 ◽  
Vol 10 (4) ◽  
pp. 280-286 ◽  
Author(s):  
Guillermo E. Madico ◽  
Peter A. Rice
Keyword(s):  
Ribosomal Dna ◽  
16S Ribosomal Dna ◽  
Culture Negative

scholarly journals The Aquatic Environment as a Reservoir ofVibrio choleraeO1 in Hydrographic Basins of the State of Pernambuco, Brazil

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Carina Lucena Mendes-Marques ◽  
Vladimir da Mota Silveira Filho ◽  
Ana Paula Rocha da Costa ◽  
Mariana de Lira Nunes ◽  
Sandoval Vieira da Silva Filho ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Virulence Genes ◽  
Intergenic Spacer ◽  
Pulsed Field Gel Electrophoresis ◽  
N Gene ◽  
Intergenic Spacer Region ◽  
Quiescent Period ◽  
Pathogenic Genes ◽  
Culture Negative

After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were alsoctxA (cholera toxin) positive. From May to June 2012, 30V. choleraeO1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for therfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemicV. choleraeO1 in the region.

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