Direct Evidence by DNA Fingerprinting that Endoscopic Cross-Infection of Helicobacter pylori Is a Cause of Postendoscopic Acute Gastritis

Helicobacter pylori infection could cause chronic inflammation in the stomach and induce peptic ulcer disease or even malignant tumour. The initial infection of the organism happens in childhood but most of cases are latent. We had a case of 10-year-old girl who presented with acute epigastric pain and significant thickening of the stomach wall on CT. The finding seemed atypical for acute gastritis so oesophagogastroduodenoscopy and serology examination were added and the primary infection of H. pylori was confirmed with the exclusion of other possible diagnoses like eosinophilic gastritis and IgA vasculitis. Acute gastritis is one of the most common sickness in children, however, it would be worthwhile considering further investigation including H. pylori infection in a case of atypical presentation to prevent negative consequences derived from chronic H. pylori infection.

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Incidence and importance of streptococci in an institution for rheumatic children

Journal of Hygiene ◽

10.1017/s002217240001559x ◽

1953 ◽

Vol 51 (2) ◽

pp. 157-161 ◽

Cited By ~ 1

Author(s):

S. A. Doxiadis ◽

Sheila M. Stewart

Keyword(s):

Direct Evidence ◽

Medical Research Council ◽

Cross Infection ◽

Number Of Children ◽

Erythrocyte Sedimentation ◽

Laboratory Facilities ◽

Nasal Swabs ◽

Group A ◽

Low Incidence ◽

Hospital School

1. The incidence of haemolytic streptococci in the patients of a Hospital School for rheumatic children was determined over a period of 1 year.2. In the total of 1644 throat and 1644 nasal swabs, group A strains were isolated from 20 throat and two nasal swabs. Eighty-seven strains of Lancefield's groups C and G and 54 strains of groups other than A, C, or G were isolated3. There was no evidence of cross-infection.4. Factors contributing to the low incidence of group A strains and the absence of cross-infection are discussed.5. The carriage of groups C and G strains did not appreciably affect the erythrocyte sedimentation rate.6. Although no direct evidence is available, the experience of the present study suggests that the incidence of streptococci in an institution for rheumatic children may be reduced by: (a) bacteriological screening before admission; (b) regular swabbing and Lancefield's grouping of streptococci isolated for the early detection of group A strains; and (c) administration of penicillin or sulphadiazine prophylactically to even a limited number of children.We thank Prof. R. S. Illingworth for his advice in the writing of this paper, Dr J. L. Emery for laboratory facilities, Dr R. E. O. Williams for his advice in the planning of the investigation, and the matron and sisters of Ash House Hospital School, Sheffield, for their assistance.The expenses of the investigation were defrayed by a grant from the Medical Research Council.

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Effectiveness of Enterobacterial Repetitive Intergenic Consensus PCR and Random Amplified Polymorphic DNA Fingerprinting for Helicobacter pylori Strain Differentiation

Applied and Environmental Microbiology ◽

10.1128/aem.00894-06 ◽

2006 ◽

Vol 72 (7) ◽

pp. 4713-4716 ◽

Cited By ~ 11

Author(s):

S. Alison Finger ◽

Billie Velapatiño ◽

Margaret Kosek ◽

Livia Santivañez ◽

Daiva Dailidiene ◽

...

Keyword(s):

Helicobacter Pylori ◽

Gene Transfer ◽

Dna Fingerprinting ◽

Efficient Method ◽

Random Amplified Polymorphic Dna ◽

Kappa Statistic ◽

Enterobacterial Repetitive Intergenic Consensus ◽

Strain Differentiation ◽

H Pylori ◽

Fingerprinting Methods

ABSTRACT We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting cases of mixed Helicobacter pylori infection in Peruvian shantytown residents. H. pylori isolates from 63 participants were cultured, and five single colonies and a pool of additional colonies from each participant were analyzed by ERIC-PCR and by RAPD tests with four 10-nucleotide primers (one primer per reaction). There was 94% agreement between the ERIC and RAPD profiles in classifying sets of isolates as uniform versus closely related but not identical versus probably unrelated, indicating a high kappa statistic of 0.8942. Subtle differences in related ERIC or RAPD patterns likely reflect gene transfer between strains, recombination, and/or mutation, whereas markedly different patterns reflect infection by unrelated strains. At least half of infected shantytown residents seemed to carry more than one H. pylori strain, although in 19 of 31 persons, the strains were closely related. Three RAPD tests, each with a different primer, were needed to achieve the sensitivity of one ERIC test. ERIC-PCR constitutes a resource- and time-efficient method for H. pylori strain differentiation.

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Direct DNA fingerprinting of Helicobacter pylori in dental plaque by PCR amplification and restriction analysis of urease a gene sequences

Serodiagnosis and Immunotherapy in Infectious Disease ◽

10.1016/0888-0786(94)90032-9 ◽

1994 ◽

Vol 6 (4) ◽

pp. 196-202 ◽

Cited By ~ 1

Author(s):

RJ Owen ◽

A Hurtado ◽

N Banatvala ◽

RA Feldman ◽

JM Hardie

Keyword(s):

Helicobacter Pylori ◽

Dna Fingerprinting ◽

Dental Plaque ◽

Restriction Analysis ◽

Pcr Amplification ◽

Gene Sequences

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Use of telomere fingerprinting to identify clonal lineages of Colletotrichum fioriniae in Kentucky mixed-fruit orchards

Plant Disease ◽

10.1094/pdis-08-20-1713-sc ◽

2021 ◽

Author(s):

Madison Julia McCulloch ◽

Nicole A Ward Gauthier ◽

Lisa Vaillancourt

Keyword(s):

Direct Evidence ◽

Test Group ◽

Cross Infection ◽

Fruit Rot ◽

Individual Species ◽

Pathogen Population ◽

Fruit Species ◽

Close Proximity ◽

Multiple Species ◽

Fruit Orchards

Multiple species in the fungal genus Colletotrichum cause anthracnose fruit rot diseases that are responsible for major yield losses of as much as 100%. Individual species of Colletotrichum typically have broad host ranges and can infect multiple fruit species. Colletotrichum fioriniae causes anthracnose fruit rots of apples, blueberries and strawberries in Kentucky orchards where these fruits grow in close proximity. This raises the possibility of cross-infection, which may have significant management implications. The potential occurrence of cross-infection was investigated by using telomere fingerprinting to identify C. fioriniae clones in several mixed-fruit orchards. Telomere fingerprints were highly polymorphic among a test group of C. fioriniae strains and effectively defined clonal lineages. Fingerprints were compared among apple, blueberry and strawberry isolates of C. fioriniae from three different orchards and similarity matrices were calculated to build phylograms for each orchard group. Multiple clonal lineages of C. fioriniae were identified within each orchard on the same fruit host. Related lineages were found among isolates from different hosts, but the results did not provide direct evidence for cross-infection of different fruit species by the same clones. Recovery of the same clonal lineages within orchards across multiple years suggested that local dispersal was important in pathogen population structure and that C. fioriniae strains persisted within orchards over time. Isolates from blueberry were less diverse than isolates from apple, perhaps related to more intensive anthracnose management protocols on apple versus blueberry. Telomere fingerprinting is a valuable tool for understanding population dynamics of Colletotrichum fruit rot fungi.

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