scholarly journals Molecular Typing of Salmonella enterica Serovar Typhi Isolates from Various Countries in Asia by a Multiplex PCR Assay on Variable-Number Tandem Repeats

2003 ◽  
Vol 41 (9) ◽  
pp. 4388-4394 ◽  
Author(s):  
Y. Liu ◽  
M.-A. Lee ◽  
E.-E. Ooi ◽  
Y. Mavis ◽  
A.-L. Tan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Molecular Typing ◽  
Salmonella Enterica ◽  
Tandem Repeats ◽  
Variable Number ◽  
Pcr Assay ◽  
Salmonella Enterica Serovar Typhi ◽  
Multiplex Pcr Assay ◽  
Variable Number Tandem Repeats

scholarly journals VNTR molecular typing of salmonella enterica serovar typhi isolates in Kathmandu valley

1970 ◽  
Vol 2 (3) ◽  
pp. 220-223 ◽  
Author(s):  
B Acharya ◽  
G Aryal ◽  
N Banjade ◽  
S Sharma ◽  
BM Pokharel ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Salmonella Enterica ◽  
Tandem Repeats ◽  
Variable Number ◽  
Epidemiological Surveillance ◽  
Kathmandu Valley ◽  
Salmonella Enterica Serovar Typhi ◽  
Variable Number Tandem Repeats ◽  
Multiple Variants ◽  
Epidemiological Surveillance System

Background: Typhoid fever continues to be a worldwide health problem, especially in developing countries. Effective epidemiological surveillance is needed to monitor the presence and spread of disease. Materials and Methods: Variable number tandem repeats (VNTR) was performed for Salmonella enterica serovar typhi by multiplex-PCR in 28 Nepalese isolates of sporadic typhoid fever. Results: From all 28 total isolates, we could identify 12 VNTR profiles among the isolates, signifying multiple variants in circulation within the region. Conclusion: The VNTR-based typing assay for serovar typhi isolates can be used during an outbreak of enteric fever. The typing could eventually form the basis of an effective epidemiological surveillance system for developing rational strategies to control typhoid fever. DOI: http://dx.doi.org/10.3126/jpn.v2i3.6026 JPN 2012; 2(3): 220-223

scholarly journals Evaluation of a Multiplex PCR Assay (Bruce-ladder) for Molecular Typing of All Brucella Species, Including the Vaccine Strains

2008 ◽  
Vol 46 (10) ◽  
pp. 3484-3487 ◽  
Author(s):  
I. Lopez-Goni ◽  
D. Garcia-Yoldi ◽  
C. M. Marin ◽  
M. J. de Miguel ◽  
P. M. Munoz ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Molecular Typing ◽  
Brucella Species ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals A combined AFLP–multiplex PCR assay for molecular typing of Escherichia coli strains using variable bacterial interspersed mosaic elements

2004 ◽  
Vol 132 (1) ◽  
pp. 61-65 ◽  
Author(s):  
R. HORVÁTH ◽  
M. DENDIS ◽  
J. SCHLEGELOVÁ ◽  
F. RŮŽIČKA ◽  
J. BENEDÍK
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Molecular Typing ◽  
Original Method ◽  
Pcr Assay ◽  
E Coli ◽  
Promising Tool ◽  
Multiplex Pcr Assay ◽  
Chromosomal Sequences

The original method for molecular typing of E. coli strains was developed using the polymorphism in chromosomal sequences of bacterial interspersed mosaic elements (BIMEs) detected by multiplex PCR and analysed by AFLP assay. The applicability of the method in the epidemiology of E. coli was tested on a group of 524 strains of human and veterinary origin. In the studied group 18 different genotypes were detected. Significant differences were found in the frequencies of the genotypes among various groups of strains, suggesting the method could be a promising tool in the epidemiology of E. coli.

Development of a novel multiplex PCR assay for the identification of Salmonella enterica Typhimurium and Enteritidis

Food Control ◽  
2012 ◽  
Vol 27 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Bin Liu ◽  
Xiujuan Zhou ◽  
Lida Zhang ◽  
Weibing Liu ◽  
Xianlong Dan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Salmonella serovar spectrum associated with reptiles in Poland

2014 ◽  
Vol 83 (4) ◽  
pp. 287-294 ◽  
Author(s):  
Tomasz Piasecki ◽  
Klaudia Chrząstek ◽  
Alina Wieliczko
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Rapid Screening ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Large Numbers ◽  
Faecal Samples ◽  
Reptilian Species

This study aimed to evaluate the incidence ofSalmonellaisolates from a wide variety of reptiles in Poland. A total of 374 faecal samples from chelonians, lizards and snakes were collected between 2009 and 2012. The nested, two-step PCR and multiplex PCR were performed to access the incidence and to characterizeSalmonellaisolates.Salmonellastrains were found in 122 of 374 samples (32.6%). Among the different reptilian species,Salmonellastrains were found in 58 samples from lizards (38.9%), 31 samples from snakes (28.7%) and 33 samples from chelonians (28.2%). Of the total of 122 strains, 72 belonged to the speciesSalmonella entericasubsp.enterica, 20 to the speciesS.entericasubs.salamaeorS.entericasubs.houtanae. The incidence ofS.entericasubs.diarizonaeandS.entericasubs.indicawas low, constituting less than 3.5% of the examined population. The findings show that reptiles can be considered as a reservoir forSalmonellaand hence could pose a zoonotic hazard. In addition, multiplex PCR assay is a rapid, specific and easy-to-perform method and might be applied for rapid screening of large numbers ofSalmonellasamples.

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