scholarly journals Salmonella serovar spectrum associated with reptiles in Poland

2014 ◽  
Vol 83 (4) ◽  
pp. 287-294 ◽  
Author(s):  
Tomasz Piasecki ◽  
Klaudia Chrząstek ◽  
Alina Wieliczko
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Rapid Screening ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Large Numbers ◽  
Faecal Samples ◽  
Reptilian Species

This study aimed to evaluate the incidence ofSalmonellaisolates from a wide variety of reptiles in Poland. A total of 374 faecal samples from chelonians, lizards and snakes were collected between 2009 and 2012. The nested, two-step PCR and multiplex PCR were performed to access the incidence and to characterizeSalmonellaisolates.Salmonellastrains were found in 122 of 374 samples (32.6%). Among the different reptilian species,Salmonellastrains were found in 58 samples from lizards (38.9%), 31 samples from snakes (28.7%) and 33 samples from chelonians (28.2%). Of the total of 122 strains, 72 belonged to the speciesSalmonella entericasubsp.enterica, 20 to the speciesS.entericasubs.salamaeorS.entericasubs.houtanae. The incidence ofS.entericasubs.diarizonaeandS.entericasubs.indicawas low, constituting less than 3.5% of the examined population. The findings show that reptiles can be considered as a reservoir forSalmonellaand hence could pose a zoonotic hazard. In addition, multiplex PCR assay is a rapid, specific and easy-to-perform method and might be applied for rapid screening of large numbers ofSalmonellasamples.

Development of a novel multiplex PCR assay for the identification of Salmonella enterica Typhimurium and Enteritidis

Food Control ◽  
2012 ◽  
Vol 27 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Bin Liu ◽  
Xiujuan Zhou ◽  
Lida Zhang ◽  
Weibing Liu ◽  
Xianlong Dan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Multiplex-PCR Assay for Detection of some Major Virulence Genes of Salmonella enterica Serovars from Diverse Sources

2016 ◽  
Vol 111 (7) ◽  
pp. 1252 ◽  
Author(s):  
Mridusmita Choudhury ◽  
Probodh Borah ◽  
Hridip Kumar Sarma ◽  
Luit Moni Barkalita ◽  
Naba Kumar Deka ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Virulence Genes ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

A one step multiplex PCR assay for rapid screening of East Asian mtDNA haplogroups on forensic samples

2013 ◽  
Vol 15 (1) ◽  
pp. 50-54 ◽  
Author(s):  
Hwan Young Lee ◽  
Jung Ah Yoon ◽  
Woo Ick Yang ◽  
Kyoung-Jin Shin
Keyword(s):  
Multiplex Pcr ◽  
East Asian ◽  
Rapid Screening ◽  
Pcr Assay ◽  
Mtdna Haplogroups ◽  
Multiplex Pcr Assay ◽  
Forensic Samples ◽  
One Step

Identification of Salmonella enterica Typhimurium and variants using a novel multiplex PCR assay

Food Control ◽  
2016 ◽  
Vol 65 ◽  
pp. 152-159 ◽  
Author(s):  
Xiaohua He ◽  
Xuebin Xu ◽  
Ke Li ◽  
Bin Liu ◽  
Tianli Yue
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Detection of Salmonella enterica serovars in shrimps in eight hours by multiplex PCR assay

2011 ◽  
Vol 62 (1) ◽  
pp. 225-231 ◽  
Author(s):  
Geevaretnam Jeyasekaran ◽  
Kannan Thirumalai Raj ◽  
Robinsondhas Jeya Shakila ◽  
Albin Jemila Thangarani ◽  
Durai Raj Sukumar
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

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