scholarly journals A Novel Bipartite Double-Stranded RNA Mycovirus from the White Root Rot Fungus Rosellinia necatrix: Molecular and Biological Characterization, Taxonomic Considerations, and Potential for Biological Control

2009 ◽  
Vol 83 (24) ◽  
pp. 12801-12812 ◽  
Author(s):  
Sotaro Chiba ◽  
Lakha Salaipeth ◽  
Yu-Hsin Lin ◽  
Atsuko Sasaki ◽  
Satoko Kanematsu ◽  
...  
Keyword(s):  
Biological Control ◽  
Root Rot ◽  
Sequence Similarity ◽  
Peptide Mass Fingerprinting ◽  
Dsrna Virus ◽  
Rosellinia Necatrix ◽  
Virus Family ◽  
White Root Rot ◽  
Double Stranded Rna ◽  
Peptide Mass

ABSTRACT White root rot, caused by the ascomycete Rosellinia necatrix, is a devastating disease worldwide, particularly in fruit trees in Japan. Here we report on the biological and molecular properties of a novel bipartite double-stranded RNA (dsRNA) virus encompassing dsRNA-1 (8,931 bp) and dsRNA-2 (7,180 bp), which was isolated from a field strain of R. necatrix, W779. Besides the strictly conserved 5′ (24 nt) and 3′ (8 nt) terminal sequences, both segments show high levels of sequence similarity in the long 5′ untranslated region of approximately 1.6 kbp. dsRNA-1 and -2 each possess two open reading frames (ORFs) named ORF1 to -4. Although the protein encoded by 3′-proximal ORF2 on dsRNA-1 shows sequence identities of 22 to 32% with RNA-dependent RNA polymerases from members of the families Totiviridae and Chrysoviridae, the remaining three virus-encoded proteins lack sequence similarities with any reported mycovirus proteins. Phylogenetic analysis showed that the W779 virus belongs to a separate clade distinct from those of other known mycoviruses. Purified virions ∼50 nm in diameter consisted of dsRNA-1 and -2 and a single major capsid protein of 135 kDa, which was shown by peptide mass fingerprinting to be encoded by dsRNA-1 ORF1. We developed a transfection protocol using purified virions to show that the virus was responsible for reduction of virulence and mycelial growth in several host strains. These combined results indicate that the W779 virus is a novel bipartite dsRNA virus with potential for biological control (virocontrol), named Rosellinia necatrix megabirnavirus 1 (RnMBV1), that possibly belongs to a new virus family.

Presence and distribution of double-stranded RNA elements in the white root rot fungus Rosellinia necatrix

Mycoscience ◽  
2002 ◽  
Vol 43 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Masao Arakawa ◽  
Hitoshi Nakamura ◽  
Yukari Uetake ◽  
Naoyuki Matsumoto
Keyword(s):  
Root Rot ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Double Stranded Rna ◽  
Rna Elements

scholarly journals Weed Roots Facilitate the Spread of Rosellinia necatrix, the Causal Agent of White Root Rot

2019 ◽  
Vol 34 (3) ◽  
pp. 340-343
Author(s):  
Hayato Shiragane ◽  
Toshiyuki Usami ◽  
Masahiro Shishido
Keyword(s):  
Root Rot ◽  
Causal Agent ◽  
Rosellinia Necatrix ◽  
White Root Rot

scholarly journals Response of the Biocontrol AgentPseudomonas pseudoalcaligenesAVO110 toRosellinia necatrixExudate

2018 ◽  
Vol 85 (3) ◽  
Author(s):  
Clara Pliego ◽  
José Ignacio Crespo-Gómez ◽  
Adrián Pintado ◽  
Isabel Pérez-Martínez ◽  
Antonio de Vicente ◽  
...  
Keyword(s):  
Root Rot ◽  
Molecular Mechanisms ◽  
Dna Helicase ◽  
Root Tip ◽  
Rosellinia Necatrix ◽  
Pseudomonas Pseudoalcaligenes ◽  
White Root Rot ◽  
Content Type ◽  
Root Rot Disease ◽  
Rot Disease

ABSTRACTThe rhizobacteriumPseudomonas pseudoalcaligenesAVO110, isolated by the enrichment of competitive avocado root tip colonizers, controls avocado white root rot disease caused byRosellinia necatrix. Here, we applied signature-tagged mutagenesis (STM) during the growth and survival of AVO110 in fungal exudate-containing medium with the goal of identifying the molecular mechanisms linked to the interaction of this bacterium withR. necatrix. A total of 26 STM mutants outcompeted by the parental strain in fungal exudate, but not in rich medium, were selected and namedgrowth-attenuatedmutants (GAMs). Twenty-one genes were identified as being required for this bacterial-fungal interaction, including membrane transporters, transcriptional regulators, and genes related to the metabolism of hydrocarbons, amino acids, fatty acids, and aromatic compounds. The bacterial traits identified here that are involved in the colonization of fungal hyphae include proteins involved in membrane maintenance (a dynamin-like protein and ColS) or cyclic-di-GMP signaling and chemotaxis. In addition, genes encoding a DNA helicase (recB) and a regulator of alginate production (algQ) were identified as being required for efficient colonization of the avocado rhizosphere.IMPORTANCEDiseases associated with fungal root invasion cause a significant loss of fruit tree production worldwide. The bacteriumPseudomonas pseudoalcaligenesAVO110 controls avocado white root rot disease caused byRosellinia necatrixby using mechanisms involving competition for nutrients and niches. Here, a functional genomics approach was conducted to identify the bacterial traits involved in the interaction with this fungal pathogen. Our results contribute to a better understanding of the multitrophic interactions established among bacterial biocontrol agents, the plant rhizosphere, and the mycelia of soilborne pathogens.

scholarly journals Appearance of mycovirus-like double-stranded RNAs in the white root rot fungus,Rosellinia necatrix, in an apple orchard

2012 ◽  
Vol 83 (1) ◽  
pp. 49-62 ◽  
Author(s):  
Hajime Yaegashi ◽  
Hitoshi Nakamura ◽  
Takuo Sawahata ◽  
Atsuko Sasaki ◽  
Yasuhiko Iwanami ◽  
...  
Keyword(s):  
Root Rot ◽  
Apple Orchard ◽  
Rosellinia Necatrix ◽  
White Root Rot

scholarly journals First Report of White Root Rot Caused by Rosellinia necatrix on Persea americana in South Africa

Plant Disease ◽  
2018 ◽  
Vol 102 (9) ◽  
pp. 1850 ◽  
Author(s):  
N. van den Berg ◽  
J. Hartley ◽  
J. Engelbrecht ◽  
Z. Mufamadi ◽  
Z. van Rooyen ◽  
...  
Keyword(s):  
South Africa ◽  
Root Rot ◽  
Persea Americana ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
First Report

scholarly journals Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis

2019 ◽  
Author(s):  
Adela Zumaquero ◽  
Satoko Kanematsu ◽  
Hitoshi Nakayashiki ◽  
Antonio Matas ◽  
Elsa Martínez-Ferri ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Root Rot ◽  
Molecular Mechanisms ◽  
Plant Cell Wall ◽  
Infection Process ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Host Interaction ◽  
Rot Disease

Abstract Background White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado `Dusa´ roots with that obtained from the fungus cultured in rich medium. Results The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1,937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. Conclusions The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of different compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among distinct plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.

scholarly journals First Report of Rosellinia necatrix Causing White Root Rot in Mango Trees in Spain

Plant Disease ◽  
2018 ◽  
Vol 102 (12) ◽  
pp. 2639 ◽  
Author(s):  
I. Arjona-Girona ◽  
C. J. López-Herrera
Keyword(s):  
Root Rot ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
First Report
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