Suppression of multiplication of avian sarcoma virus by rapid spread of transformation-defective virus of the same subgroup.

1979 ◽  
Vol 31 (2) ◽  
pp. 389-397 ◽  
Author(s):  
L F Estis ◽  
H M Temin
Keyword(s):  
Avian Sarcoma Virus ◽  
Rapid Spread ◽  
Sarcoma Virus ◽  
Avian Sarcoma ◽  
Defective Virus

Transformation-Defective Virus Generation from Cloned Nondefective Avian Sarcoma Virus

Intervirology ◽  
1980 ◽  
Vol 14 (5-6) ◽  
pp. 326-330 ◽  
Author(s):  
Stephen E. Wright ◽  
Shirley A. Harmon ◽  
William W. Lamph
Keyword(s):  
Avian Sarcoma Virus ◽  
Sarcoma Virus ◽  
Avian Sarcoma ◽  
Defective Virus

Avian sarcoma virus-transformed quail clones defective in the production of focus-forming virus.

1979 ◽  
Vol 30 (1) ◽  
pp. 132-140 ◽  
Author(s):  
W S Mason ◽  
T W Hsu ◽  
C Yeater ◽  
J L Sabran ◽  
G E Mark ◽  
...  
Keyword(s):  
Avian Sarcoma Virus ◽  
Sarcoma Virus ◽  
Avian Sarcoma

Towards a molecular description of cell transformation by avian sarcoma virus

1980 ◽  
Vol 210 (1180) ◽  
pp. 387-396 ◽  
Keyword(s):  
Protein Kinase ◽  
Protein Phosphorylation ◽  
Molecular Mass ◽  
Cellular Protein ◽  
Transformed Cells ◽  
Relative Molecular Mass ◽  
Protein Kinase Activity ◽  
Avian Sarcoma Virus ◽  
Sarcoma Virus ◽  
Avian Sarcoma

The avian sarcoma virus transforming gene product has been identified and partially purified from extracts of transformed cells. It is a phosphoprotein with a relative molecular mass of 60 000 (pp60 src ) with two major sites of phosphorylation. pp60 src appears to be a cyclic-AMP-independent protein kinase as judged by protein phosphorylation with partly purified fractions. The specificity of the phosphorylation observed was judged by inhibition with anti-pp60 src IgG but not by normal IgG and by the fact that the protein kinase activity isolated from ts transformation-mutant infected cells was more thermolabile than that from wild-type transformed cells, thus showing more directly the origin of the enzymic activity. A cellular protein substrate of pp60 src has been identified as a 34000 molecular mass protein. These data together suggest that protein phosphorylation by pp60 src may be a function of the molecule that plays a major role in transformation.

Localization of active promoters for eucaryotic RNA polymerase II in the long terminal repeat of avian sarcoma virus DNA

1983 ◽  
Vol 3 (5) ◽  
pp. 811-818
Author(s):  
S A Mitsialis ◽  
J L Manley ◽  
R V Guntaka
Keyword(s):  
Rna Polymerase ◽  
Hela Cell ◽  
Long Terminal Repeat ◽  
Rna Polymerase Ii ◽  
Terminal Repeat ◽  
Avian Sarcoma Virus ◽  
Cell Extracts ◽  
Sarcoma Virus ◽  
Avian Sarcoma

The nucleotide sequences in the long terminal repeat of avian sarcoma virus that are recognized in vitro by HeLa cell RNA polymerase II have been identified. For this purpose, various 5' and 3' deletions were introduced into a cloned long terminal repeat fragment. The effects of these deletions on transcription initiation in HeLa whole-cell extracts were then studied. Three specific transcripts have been identified. The major transcript is initiated at nucleotide +1 (relative to the cap site). Deletion of the upstream sequence between -299 and -55 has no effect on the level of transcription from this start site, whereas deletion of the sequence downstream of -14 drastically reduces the levels of transcription. In contrast, deletion of the sequence downstream from the TATA box has no effect on the initiation or efficiency of synthesis of the two minor RNA species, which are initiated at around nucleotides -260 and -105. The transcription of these RNA products, however, is abolished by an upstream deletion between -299 and -55. These results suggest that HeLa cell RNA polymerase II recognizes in vitro more than one promoter site present in the long terminal repeat of the avian sarcoma virus genome and defines the sequences required for initiation of the major transcript.

Cell-free translation of avian sarcoma virus RNA: Suppression of the gag termination codon does not augment synthesis of the joint gag/pol Product

Cell ◽  
1978 ◽  
Vol 15 (2) ◽  
pp. 607-614 ◽  
Author(s):  
Susan R. Weiss ◽  
Perry B. Hackett ◽  
Hermann Oppermann ◽  
Axel Ullrich ◽  
Leon Levintow ◽  
...  
Keyword(s):  
Termination Codon ◽  
Avian Sarcoma Virus ◽  
Virus Rna ◽  
Free Translation ◽  
Sarcoma Virus ◽  
Avian Sarcoma
Sign in / Sign up

Export Citation Format

Share Document