Complex of simian virus 40 large-T antigen and host 53,000-molecular-weight protein in monkey cells.

1981 ◽  
Vol 37 (2) ◽  
pp. 564-573 ◽  
Author(s):  
E Harlow ◽  
D C Pim ◽  
L V Crawford
Keyword(s):  
Molecular Weight ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Molecular Weight Protein ◽  
Large T Antigen ◽  
Weight Protein

Two integrated partial repeats of simian virus 40 together code for a super-T antigen

1985 ◽  
Vol 5 (4) ◽  
pp. 742-750
Author(s):  
A Levitt ◽  
S Chen ◽  
G Blanck ◽  
D George ◽  
R E Pollack
Keyword(s):  
Molecular Weight ◽  
Control Region ◽  
Viral Genome ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Differential Splicing ◽  
Coding Sequence ◽  
Gene Copies

We determined that the coding sequence for a 100-kilodalton super-T antigen found in Simian virus 40 mouse transformants spanned two separate partial repeats of the viral genome. The downstream repeat contained a complete Simian virus 40 large-T-antigen gene, whereas the upstream repeat was a truncated copy of the same gene. When the repeats were separated by subcloning, the capacity to code for the super-T antigen was lost. A small insertion or deletion in the origin-control region which preceded the second repeat could also destroy the ability to code for the 100-kilodalton protein. Our data suggest that differential splicing between parts of two gene copies was responsible for the additional molecular weight of this super-T antigen.

scholarly journals Two integrated partial repeats of simian virus 40 together code for a super-T antigen.

1985 ◽  
Vol 5 (4) ◽  
pp. 742-750 ◽  
Author(s):  
A Levitt ◽  
S Chen ◽  
G Blanck ◽  
D George ◽  
R E Pollack
Keyword(s):  
Molecular Weight ◽  
Control Region ◽  
Viral Genome ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Differential Splicing ◽  
Coding Sequence ◽  
Gene Copies

We determined that the coding sequence for a 100-kilodalton super-T antigen found in Simian virus 40 mouse transformants spanned two separate partial repeats of the viral genome. The downstream repeat contained a complete Simian virus 40 large-T-antigen gene, whereas the upstream repeat was a truncated copy of the same gene. When the repeats were separated by subcloning, the capacity to code for the super-T antigen was lost. A small insertion or deletion in the origin-control region which preceded the second repeat could also destroy the ability to code for the 100-kilodalton protein. Our data suggest that differential splicing between parts of two gene copies was responsible for the additional molecular weight of this super-T antigen.

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