scholarly journals Human T cells recovered from human/Balb radiation chimeras are hypersensitive to human immunodeficiency virus type 1 infection.

1997 ◽  
Vol 71 (6) ◽  
pp. 4495-4501 ◽  
Author(s):  
O Shapira-Nahor ◽  
H Marcus ◽  
H Segall ◽  
I Lubin ◽  
S Slavin ◽  
...  
1996 ◽  
Vol 40 (11) ◽  
pp. 827-835 ◽  
Author(s):  
Yukako Ohshiro ◽  
Tsutomu Murakami ◽  
Kazuhiro Matsuda ◽  
Kiyoshi Nishioka ◽  
Keiichi Yoshida ◽  
...  

2004 ◽  
Vol 78 (20) ◽  
pp. 11352-11359 ◽  
Author(s):  
Yuji Kawano ◽  
Takeshi Yoshida ◽  
Kuniko Hieda ◽  
Jun Aoki ◽  
Hiroyuki Miyoshi ◽  
...  

ABSTRACT Expression cloning technology of cDNAs is a suitable tool for identifying novel functional properties of genes. Here, we generated a lentiviral cDNA library-expressing system for human T cells based on a site-specific recombination system of phage lambda for transferring cDNA libraries with a minimum loss of its complexity. The library-transduced CD4+ T cells were challenged with wild-type human immunodeficiency virus type 1 (HIV-1), and the cells that acquired resistance to HIV-1-induced cytopathic effect (CPE) were selected. From these cells, CD14 was isolated and proved to inhibit the entry of HIV-1 and the HIV-1-induced CPE. This cloning system allows rapid identification of genes encoding novel properties in human T cells and probably other mammalian cells.


1997 ◽  
Vol 71 (7) ◽  
pp. 5259-5267 ◽  
Author(s):  
J H Simon ◽  
R A Fouchier ◽  
T E Southerling ◽  
C B Guerra ◽  
C K Grant ◽  
...  

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