scholarly journals The Role of Integration Host Factor in Escherichia coli Persister Formation

mBio ◽  
2022 ◽  
Author(s):  
Samantha E. Nicolau ◽  
Kim Lewis
Keyword(s):  
Escherichia Coli ◽  
Treatment Failure ◽  
Antibiotic Treatment ◽  
Integration Host Factor ◽  
Host Factor ◽  
Clinical Isolates ◽  
Chronic Infections

Persisters are phenotypic variants that survive exposure to antibiotics through temporary dormancy. Mutants with increased levels of persisters have been identified in clinical isolates, and evidence suggests these cells contribute to chronic infections and antibiotic treatment failure.

scholarly journals The role of integration host factor In gene expression in Escherichia coli

1992 ◽  
Vol 6 (18) ◽  
pp. 2557-2563 ◽  
Author(s):  
M. Freundlich ◽  
N. Ramani ◽  
E. Mathew ◽  
A. Sirko ◽  
P. Tsui
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Integration Host Factor ◽  
Host Factor ◽  
Expression In Escherichia Coli

scholarly journals The Escherichia coli K-12 NarL and NarP Proteins Insulate the nrf Promoter from the Effects of Integration Host Factor

2006 ◽  
Vol 188 (21) ◽  
pp. 7449-7456 ◽  
Author(s):  
Douglas F. Browning ◽  
David J. Lee ◽  
Alan J. Wolfe ◽  
Jeffrey A. Cole ◽  
Stephen J. W. Busby
Keyword(s):  
Escherichia Coli ◽  
Response Regulator ◽  
Regulatory Region ◽  
Enteric Bacteria ◽  
Integration Host Factor ◽  
Host Factor ◽  
Receptor Protein ◽  
E Coli ◽  
Serovar Typhimurium ◽  
K 12

ABSTRACT The Escherichia coli K-12 nrf operon promoter can be activated fully by the FNR protein (regulator of fumarate and nitrate reduction) binding to a site centered at position −41.5. FNR-dependent transcription is suppressed by integration host factor (IHF) binding at position −54, and this suppression is counteracted by binding of the NarL or NarP response regulator at position −74.5. The E. coli acs gene is transcribed from a divergent promoter upstream from the nrf operon promoter. Transcription from the major acsP2 promoter is dependent on the cyclic AMP receptor protein and is modulated by IHF and Fis binding at multiple sites. We show that IHF binding to one of these sites, located at position −127 with respect to the nrf promoter, has a positive effect on nrf promoter activity. This activation is dependent on the face of the DNA helix, independent of IHF binding at other locations, and found only when NarL/NarP are not bound at position −74.5. Binding of NarL/NarP appears to insulate the nrf promoter from the effects of IHF. The acs-nrf regulatory region is conserved in other pathogenic E. coli strains and related enteric bacteria but differs in Salmonella enterica serovar Typhimurium.

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