scholarly journals Mutations in ABO1/ELO2, a Subunit of Holo-Elongator, Increase Abscisic Acid Sensitivity and Drought Tolerance in Arabidopsis thaliana

2006 ◽  
Vol 26 (18) ◽  
pp. 6902-6912 ◽  
Author(s):  
Zhizhong Chen ◽  
Hairong Zhang ◽  
Daniel Jablonowski ◽  
Xiaofeng Zhou ◽  
Xiaozhi Ren ◽  
...  

ABSTRACT The phytohormone abscisic acid (ABA) plays an important role in modulating plant growth, development, and stress responses. In a genetic screen for mutants with altered drought stress responses, we identified an ABA-overly sensitive mutant, the abo1 mutant, which showed a drought-resistant phenotype. The abo1 mutation enhances ABA-induced stomatal closing and increases ABA sensitivity in inhibiting seedling growth. abo1 mutants are more resistant to oxidative stress than the wild type and show reduced levels of transcripts of several stress- or ABA-responsive genes. Interestingly, the mutation also differentially modulates the development and growth of adjacent guard cells. Map-based cloning identified ABO1 as a new allele of ELO2, which encodes a homolog of Saccharomyces cerevisiae Iki3/Elp1/Tot1 and human IκB kinase-associated protein. Iki3/Elp1/Tot1 is the largest subunit of Elongator, a multifunctional complex with roles in transcription elongation, secretion, and tRNA modification. Ecotopic expression of plant ABO1/ELO2 in a tot1/elp1Δ yeast Elongator mutant complements resistance to zymocin, a yeast killer toxin complex, indicating that ABO1/ELO2 substitutes for the toxin-relevant function of yeast Elongator subunit Tot1/Elp1. Our results uncover crucial roles for ABO1/ELO2 in modulating ABA and drought responses in Arabidopsis thaliana.

2021 ◽  
Author(s):  
Li Qing ◽  
Tian Qianqian ◽  
Zhang Yue ◽  
Niu Mengxue ◽  
Yu Xiaoqian ◽  
...  

Abstract Abscisic acid (ABA) is a key plant hormone that regulates plant growth development and stress response. ABA is recognized and bound by ABA Receptor PYR/PYL/RCAR (referred to as PYLs). However, little is known about the PYLs gene family in Populus euphratica. Here, we identified 12 PYLs in P. euphratica and named PePYL1-12. Phylogenetic analysis divided the 12 PePYLs into three subfamilies. Subcellular localization showed that PePYL2, PePYL4, PePYL5, PePYL6, and PePYL9 were located in the cytoplasm and nucleus, PePYL10 localized in the nucleus. The promoter of 12 PePYLs contains hormones- and abiotic stress-related cis-acting elements. Moreover, ABA and drought significantly up-regulation the expression of PePYL6 and PePYL9. To study the performance of PePYLs under ABA and drought stress, we generated transgenic Arabidopsis plants overexpressing PePYL6 and PePYL9. Compared with wild type, transgenic Arabidopsis enhanced ABA sensitivity during seed germination and root growth, improved water use efficiency and drought resistance. Taken together, our results confirmed that PePYL6 and PePYL9 play a positive role in ABA-mediated stress responses in P. euphratica.


2019 ◽  
Vol 21 (1) ◽  
pp. 26
Author(s):  
Hua-feng Zhang ◽  
Su-ya Liu ◽  
Ji-hui Ma ◽  
Xin-ke Wang ◽  
Saeed ul Haq ◽  
...  

Dehydrins play an important role in improving plant resistance to abiotic stresses. In this study, we isolated a dehydrin gene from pepper (Capsicum annuum L.) leaves, designated as CaDHN4. Sub-cellular localization of CaDHN4 was to be found in the nucleus and membrane. To authenticate the function of CaDHN4 in cold- and salt-stress responses and abscisic acid (ABA) sensitivity, we reduced the CaDHN4 expression using virus-induced gene silencing (VIGS), and overexpressed the CaDHN4 in Arabidopsis. We found that silencing of CaDHN4 reduced the growth of pepper seedlings and CaDHN4-silenced plants exhibited more serious wilting, higher electrolyte leakage, and more accumulation of ROS in the leaves compared to pTRV2:00 plants after cold stress, and lower chlorophyll contents and higher electrolyte leakage compared to pTRV2:00 plants under salt stress. However, CaDHN4-overexpressing Arabidopsis plants had higher seed germination rates and post-germination primary root growth, compared to WT plants under salt stress. In response to cold and salt stresses, the CaDHN4-overexpressed Arabidopsis exhibited lower MDA content, and lower relative electrolyte leakage compared to the WT plants. Under ABA treatments, the fresh weight and germination rates of transgenic plants were higher than WT plants. The transgenic Arabidopsis expressing a CaDHN4 promoter displayed a more intense GUS staining than the normal growth conditions under treatment with hormones including ABA, methyl jasmonate (MeJA), and salicylic acid (SA). Our results suggest that CaDHN4 can protect against cold and salt stresses and decrease ABA sensitivity in Arabidopsis.


1988 ◽  
pp. 216-216
Author(s):  
L. Polonelli ◽  
M. Castagnola ◽  
G. Morace

2009 ◽  
Vol 17 (4) ◽  
pp. 552-560 ◽  
Author(s):  
Susheela Y. Carroll ◽  
Peter C. Stirling ◽  
Helen E.M. Stimpson ◽  
Esther Gießelmann ◽  
Manfred J. Schmitt ◽  
...  

1990 ◽  
Vol 110 (3) ◽  
pp. 169-175 ◽  
Author(s):  
Luciano Polonelli ◽  
Stefania Conti ◽  
Mara Gerloni ◽  
Lauresa Campani ◽  
Massimo Pettoello Mantovani ◽  
...  

1998 ◽  
Vol 177 (3) ◽  
pp. 807-811 ◽  
Author(s):  
Stefania Conti ◽  
Franco Fanti ◽  
Walter Magliani ◽  
Mara Gerloni ◽  
Daniela Bertolotti ◽  
...  

1993 ◽  
Vol 5 (2) ◽  
pp. 102-106 ◽  
Author(s):  
EM Aliouat ◽  
JC Cailliez ◽  
N Séguy ◽  
E Dei-Cas ◽  
L Polonelli ◽  
...  

1996 ◽  
Vol 37 (2) ◽  
pp. 63-69
Author(s):  
S. Conti ◽  
W. Magliani ◽  
M. Gerloni ◽  
P. Fisicaro ◽  
C. Cantelli ◽  
...  

1997 ◽  
Vol 3 (8) ◽  
pp. 544-552 ◽  
Author(s):  
Nathalie Séguy ◽  
Jean-Charles Cailliez ◽  
Philippe Delcourt ◽  
Stefania Conti ◽  
Daniel Camus ◽  
...  

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