scholarly journals Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae

1992 ◽  
Vol 12 (4) ◽  
pp. 1805-1814
Author(s):  
P Detloff ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
High Frequency ◽  
Excision Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Heteroduplex Dna ◽  
Homologous Chromosomes ◽  
His4 Gene

During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed at a high frequency between HIS4 genes located on homologous chromosomes. Using mutant alleles of the HIS4 gene that result in poorly repaired mismatches in heteroduplex DNA, we find that heteroduplexes often span a distance of 1.8 kb. In addition, we show that about one-third of the repair tracts initiated at well-repaired mismatches extend 900 bp.

scholarly journals Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae.

1992 ◽  
Vol 12 (4) ◽  
pp. 1805-1814 ◽  
Author(s):  
P Detloff ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
High Frequency ◽  
Excision Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Heteroduplex Dna ◽  
Homologous Chromosomes ◽  
His4 Gene

During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed at a high frequency between HIS4 genes located on homologous chromosomes. Using mutant alleles of the HIS4 gene that result in poorly repaired mismatches in heteroduplex DNA, we find that heteroduplexes often span a distance of 1.8 kb. In addition, we show that about one-third of the repair tracts initiated at well-repaired mismatches extend 900 bp.

scholarly journals CHROMOSOMAL TRANSLOCATIONS GENERATED BY HIGH-FREQUENCY MEIOTIC RECOMBINATION BETWEEN REPEATED YEAST GENES

Genetics ◽  
1986 ◽  
Vol 114 (3) ◽  
pp. 731-752
Author(s):  
Sue Jinks-Robertson ◽  
Thomas D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Meiotic Recombination ◽  
High Frequency ◽  
Mitotic Recombination ◽  
Chromosomal Translocations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Homologous Chromosomes ◽  
Yeast Genes ◽  
Repeated Genes

ABSTRACT We have examined meiotic and mitotic recombination between repeated genes on nonhomologous chromosomes in the yeast Saccharomyces cerevisiae . The results of these experiments can be summarized in three statements. First, gene conversion events between repeats on nonhomologous chromosomes occur frequently in meiosis. The frequency of such conversion events is only 17-fold less than the analogous frequency of conversion between genes at allelic positions on homologous chromosomes. Second, meiotic and mitotic conversion events between repeated genes on nonhomologous chromosomes are associated with reciprocal recombination to the same extent as conversion between allelic sequences. The reciprocal exchanges between the repeated genes result in chromosomal translocations. Finally, recombination between repeated genes on nonhomologous chromosomes occurs much more frequently in meiosis than in mitosis.

scholarly journals Competition Between Adjacent Meiotic Recombination Hotspots in the Yeast Saccharomyces cerevisiae

Genetics ◽  
1997 ◽  
Vol 145 (3) ◽  
pp. 661-670 ◽  
Author(s):  
Qing-Qing Fan ◽  
Fei Xu ◽  
Michael A White ◽  
Thomas D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Telomeric Sequence ◽  
Coding Region ◽  
Dna Breaks ◽  
Local Formation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Recombination Hotspots ◽  
Double Strand Dna Breaks ◽  
His4 Gene

In a wild-type strain of Saccharomyces cerevisiae, a hotspot for meiotic recombination is located upstream of the HIS4 gene. An insertion of a 49-bp telomeric sequence into the coding region of HIS4 strongly stimulates meiotic recombination and the local formation of meiosis-specific double-strand DNA breaks (DSBs). When strains are constructed in which both hotspots are heterozygous, hotspot activity is substantially less when the hotspots are on the same chromosome than when they are on opposite chromosomes.

scholarly journals Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.

1993 ◽  
Vol 13 (4) ◽  
pp. 2324-2331 ◽  
Author(s):  
D K Nag ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Physical Method ◽  
Yeast Saccharomyces Cerevisiae ◽  
Heteroduplex Dna ◽  
Dna Strands ◽  
General Physical ◽  
Palindromic Sequences ◽  
Heteroduplex Formation

We describe a general physical method for detecting the heteroduplex DNA that is formed as an intermediate in meiotic recombination in the yeast Saccharomyces cerevisiae. We use this method to study the kinetic relationship between the formation of heteroduplex DNA and other meiotic events. We show that strains with the rad50, but not the rad52, mutation are defective in heteroduplex formation. We also demonstrate that, although cruciform structures can be formed in vivo as a consequence of heteroduplex formation between DNA strands that contain different palindromic insertions, small palindromic sequences in homoduplex DNA are rarely extruded into the cruciform conformation.

scholarly journals RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination.

1990 ◽  
Vol 10 (6) ◽  
pp. 2485-2491 ◽  
Author(s):  
R H Schiestl ◽  
S Prakash
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Excision Repair ◽  
Mitotic Recombination ◽  
The Other ◽  
Gene Products ◽  
Repair Gene ◽  
Homologous Chromosomes ◽  
Intrachromosomal Recombination ◽  
Recombination Pathway

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3 delta 3' his3 delta 5'). The rate of formation of HIS3+ recombinants in the rad10 delta mutant was not affected by the rad1 delta mutation but decreased synergistically in the presence of the rad10 delta mutation in combination with the rad52 delta mutation. These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway. The rad10 delta mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RAD1 and RAD10 gene products act in recombination after the formation of the recombinogenic substrate. The rad1 delta and rad10 delta mutations did not affect meiotic intrachromosomal recombination of the his3 delta 3' his3 delta 5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.

Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae.

Genetics ◽  
1992 ◽  
Vol 132 (1) ◽  
pp. 113-123 ◽  
Author(s):  
P Detloff ◽  
M A White ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Mismatch Repair ◽  
Meiotic Recombination ◽  
Present Evidence ◽  
Homologous Chromosomes ◽  
A Site ◽  
His4 Gene ◽  
Heteroduplex Formation ◽  
Recombination Gene

Abstract Heteroduplexes formed between genes on homologous chromosomes are intermediates in meiotic recombination. In the HIS4 gene of Saccharomyces cerevisiae, most mutant alleles at the 5' end of the gene have a higher rate of meiotic recombination (gene conversion) than mutant alleles at the 3' end of the gene. Such gradients are usually interpreted as indicating a higher frequency of heteroduplex formation at the high conversion end of the gene. We present evidence indicating that the gradient of conversion at HIS4 primarily reflects the direction of mismatch repair rather than the frequency of heteroduplex formation. We also identify a site located between the 5' end of HIS4 and the 3' end of BIK1 that stimulates heteroduplex formation at HIS4 and BIK1.

scholarly journals Repair of specific base pair mismatches formed during meiotic recombination in the yeast Saccharomyces cerevisiae

1991 ◽  
Vol 11 (2) ◽  
pp. 737-745
Author(s):  
P Detloff ◽  
J Sieber ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Base Pair ◽  
Wild Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Homologous Chromosomes ◽  
Base Pair Mismatch ◽  
Dna Strands ◽  
Postmeiotic Segregation ◽  
Mutant Genes ◽  
His4 Gene

Heteroduplexes formed between DNA strands derived from different homologous chromosomes are an intermediate in meiotic crossing over in the yeast Saccharomyces cerevisiae and other eucaryotes. A heteroduplex formed between wild-type and mutant genes will contain a base pair mismatch; failure to repair this mismatch will lead to postmeiotic segregation (PMS). By analyzing the frequency of PMS for various mutant alleles in the yeast HIS4 gene, we showed that C/C mismatches were inefficiently repaired relative to all other point mismatches. These other mismatches (G/G, G/A, T/T, A/A, T/C, C/A, A/A, and T/G) were repaired with approximately the same efficiency. We found that in spores with unrepaired mismatches in heteroduplexes, the nontranscribed strand of the HIS4 gene was more frequently donated than the transcribed strand. In addition, the direction of repair for certain mismatches was nonrandom.

scholarly journals Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae

1993 ◽  
Vol 13 (4) ◽  
pp. 2324-2331 ◽  
Author(s):  
D K Nag ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Physical Method ◽  
Yeast Saccharomyces Cerevisiae ◽  
Heteroduplex Dna ◽  
Dna Strands ◽  
General Physical ◽  
Palindromic Sequences ◽  
Heteroduplex Formation

We describe a general physical method for detecting the heteroduplex DNA that is formed as an intermediate in meiotic recombination in the yeast Saccharomyces cerevisiae. We use this method to study the kinetic relationship between the formation of heteroduplex DNA and other meiotic events. We show that strains with the rad50, but not the rad52, mutation are defective in heteroduplex formation. We also demonstrate that, although cruciform structures can be formed in vivo as a consequence of heteroduplex formation between DNA strands that contain different palindromic insertions, small palindromic sequences in homoduplex DNA are rarely extruded into the cruciform conformation.

Chromosomal assignment of mutations by specific chromosome loss in the yeast Saccharomyces cerevisiae.

Genetics ◽  
1990 ◽  
Vol 125 (2) ◽  
pp. 333-340 ◽  
Author(s):  
L P Wakem ◽  
F Sherman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasmid Dna ◽  
High Frequency ◽  
Recessive Mutation ◽  
Chromosomal Assignment ◽  
Chromosome Loss ◽  
Specific Chromosome ◽  
Yeast Saccharomyces Cerevisiae ◽  
Homologous Chromosomes

Abstract Yeast 2-microns plasmids were integrated near the centromere of a different chromosome in each of 16 cir0 mapping strains of Saccharomyces cerevisiae. The specific chromosomes containing the integrated 2-microns plasmid DNA were lost at a high frequency after crossing the cir0 strains to cir+ strains. A recessive mutation in a cir+ strain can then be easily assigned to its chromosome using this set of mapping strains, since the phenotype of the recessive mutation will be manifested only in diploids having the integrated 2-microns plasmid and the unmapped mutation on homologous chromosomes.

RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination

1990 ◽  
Vol 10 (6) ◽  
pp. 2485-2491
Author(s):  
R H Schiestl ◽  
S Prakash
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Excision Repair ◽  
Mitotic Recombination ◽  
The Other ◽  
Gene Products ◽  
Repair Gene ◽  
Homologous Chromosomes ◽  
Intrachromosomal Recombination ◽  
Recombination Pathway

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3 delta 3' his3 delta 5'). The rate of formation of HIS3+ recombinants in the rad10 delta mutant was not affected by the rad1 delta mutation but decreased synergistically in the presence of the rad10 delta mutation in combination with the rad52 delta mutation. These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway. The rad10 delta mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RAD1 and RAD10 gene products act in recombination after the formation of the recombinogenic substrate. The rad1 delta and rad10 delta mutations did not affect meiotic intrachromosomal recombination of the his3 delta 3' his3 delta 5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.

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