Diverse forms of a receptor for acidic and basic fibroblast growth factors.

We recently reported the isolation of a chicken cDNA clone encoding a basic fibroblast growth factor (FGF) receptor that has three immunoglobulinlike domains in the extracellular region. We have now identified four unique human cDNA clones encoding previously unknown FGF receptor variants which contain only two immunoglobulinlike domains. Two of the human clones encode membrane-spanning receptors, and two encode putative secreted forms. Both the three- and two-immunoglobulinlike-domain forms mediate biological responsiveness to acidic and basic FGF. Thus, the first immunoglobulinlike domain of the three-domain form may have a function other than binding of acidic and basic FGF.

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Differential Regulation of Fibroblast Growth Factor (FGF) Receptor-1 mRNA and Protein by Two Molecular Forms of Basic FGF

Journal of Biological Chemistry ◽

10.1074/jbc.271.10.5663 ◽

1996 ◽

Vol 271 (10) ◽

pp. 5663-5670 ◽

Cited By ~ 39

Author(s):

Agnes Estival ◽

Veronique Monzat ◽

Karine Miquel ◽

François Gaubert ◽

Etienne Hollande ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Differential Regulation ◽

Molecular Forms ◽

Fibroblast Growth ◽

Fgf Receptor ◽

Basic Fgf

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A murine fibroblast growth factor (FGF) receptor expressed in CHO cells is activated by basic FGF and Kaposi FGF.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.87.11.4378 ◽

1990 ◽

Vol 87 (11) ◽

pp. 4378-4382 ◽

Cited By ~ 90

Author(s):

A. Mansukhani ◽

D. Moscatelli ◽

D. Talarico ◽

V. Levytska ◽

C. Basilico

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Cho Cells ◽

Fibroblast Growth ◽

Fgf Receptor ◽

Basic Fgf ◽

Murine Fibroblast

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Increase of basic fibroblast growth factor (FGF) and FGF receptor messenger RNA during rat thyroid hyperplasia: temporal changes and cellular distribution

Journal of Endocrinology ◽

10.1677/joe.0.1420325 ◽

1994 ◽

Vol 142 (2) ◽

pp. 325-338 ◽

Cited By ~ 21

Author(s):

G P Becks ◽

A Logan ◽

I D Phillips ◽

J-F Wang ◽

C Smith ◽

...

Keyword(s):

Growth Rate ◽

Body Weight ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Messenger Rna ◽

Fibroblast Growth ◽

Fgf Receptor ◽

Basic Fgf ◽

Thyroid Hyperplasia ◽

Receptor Mrnas

Abstract Goitre was induced in adult rats by acute (1 or 2 weeks) or chronic (4 or 10 weeks) administration of methimazole together with a low iodine diet. Involution of thyroid growth was then observed at 16 weeks, 4 weeks after withdrawal of goitrogens and reversion to a normal diet. Experimental animals quickly became hypothyroid compared with controls and exhibited thyroid hyperplasia (control (n=10): total serum thyroxine (T4) 66 ±4 nmol/l, thyroid weight 5 ± 1 mg/100 g body weight, means± s.d.; experimental (n=10): T4 undetectable, thyroid weight 27 ±4 mg/100 g body weight after 2 weeks of treatment). Thyroid growth rate subsequently slowed between 2 and 10 weeks. Messenger RNA for basic fibroblast growth factor (basic FGF) and for the high-affinity FGF receptor, was compared in the thyroids and livers of control and goitrous rats by ribonuclease protection assay. Low levels of mRNA for basic FGF and its receptor were detectable in thyroids from control rats at all times, while none was detected in the livers from any animal. Basic FGF and receptor mRNAs increased, and were detected at greatest abundance in hyperplastic thyroids at 1 and 2 weeks respectively, during goitre formation, but subsequently declined in parallel with thyroid growth rate at 4 and 10 weeks. When quantified by radioimmunoassay, basic FGF extracted from thyroids was fivefold greater than in controls after 1 week of goitrogen treatment (control (n=4): 24±9 pmol/μg DNA; goitre (n=4): 100± 16 pmol/μg DNA; P<0·05). Basic FGF and FGF receptor mRNAs localized by in situ hybridization predominantly to the epithelial cell population within follicles. Localization by immunohistochemistry demonstrated that basic FGF was present in the thyroids of control rats, and was largely associated with the basement membrane of follicles. During thyroid hyperplasia, increased basic FGF immunoreactivity appeared over the cytoplasm of follicular epithelial cells and was lost from the extracellular matrix. Thyroid involution following removal of goitrogen/low iodine treatment was associated with a decrease in mRNA for basic FGF or its receptor, and a loss of immunoreactive basic FGF from the cytoplasm of follicular cells. These results suggest that autocrine expression of basic FGF and FGF receptor could contribute to thyroid hyperplasia in rats. Journal of Endocrinology (1994) 142, 325–338

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Fibroblast growth factors 1 and 2 differently activate MAP kinase in Xenopus oocytes expressing fibroblast growth factor receptors 1 and 4

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ◽

10.1016/s0167-4889(01)00074-x ◽

2001 ◽

Vol 1538 (2-3) ◽

pp. 228-233 ◽

Cited By ~ 8

Author(s):

Katia Cailliau ◽

Edith Browaeys-Poly ◽

Jean Pierre Vilain

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Map Kinase ◽

Fibroblast Growth Factor ◽

Xenopus Oocytes ◽

Fibroblast Growth Factors ◽

Growth Factor Receptors ◽

Fibroblast Growth ◽

Fibroblast Growth Factor Receptors

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Localization of fibroblast growth factor-2 (basic FGF) and FGF receptor-1 in adult human kidney11See Editorial by Barasch, p. 1156.

Kidney International ◽

10.1046/j.1523-1755.1999.00637.x ◽

1999 ◽

Vol 56 (3) ◽

pp. 883-897 ◽

Cited By ~ 30

Author(s):

Jürgen Floege ◽

Kelly L. Hudkins ◽

Frank Eitner ◽

Yan Cui ◽

Richard S. Morrison ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Fibroblast Growth Factor 2 ◽

Fibroblast Growth ◽

Fgf Receptor ◽

Basic Fgf ◽

Adult Human

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Differential expression and regulation of two distinct fibroblast growth factor receptors during early development of the urodele amphibian Pleurodeles waltl

Development ◽

10.1242/dev.116.1.261 ◽

1992 ◽

Vol 116 (1) ◽

pp. 261-273 ◽

Cited By ~ 1

Author(s):

D.L. Shi ◽

J.J. Feige ◽

J.F. Riou ◽

D.W. DeSimone ◽

J.C. Boucaut

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Differential Expression ◽

Early Development ◽

Cdna Clones ◽

Mrna Levels ◽

Fibroblast Growth ◽

Tail Bud ◽

Fgf Receptor ◽

Pleurodeles Waltl

Fibroblast growth factor (FGF) has been shown to be involved in mesoderm induction during amphibian development. Its presence in the embryo suggests that FGF is an endogenous inducer. By polymerase chain reaction (PCR) methods and by screening a Pleurodeles waltl tail-bud cDNA library with a cDNA probe for human FGF receptor, we have isolated two full-length cDNA clones, which we designate PFR1 and PFR4 based on their homology to the human FGF receptors FGFR-1 and FGFR-4. Both cDNA clones encode Pleurodeles FGF receptors that share characteristics common to members of the FGF receptor superfamily. The deduced amino acid sequence of PFR1 is 85% identical overall with the human fms-like-gene (FLG). PFR4 is most closely related to the human FGFR-4 (66% overall identity). The tyrosine kinase catalytic domains of both receptors are remarkably conserved. The two receptors show distinct patterns of regulation during early development. PFR1 first appears as a maternally derived mRNA and mRNA levels remain constant during early developmental stages. However, PFR4 mRNA is first expressed at the late blastula stage, which suggests that its expression is a result of zygotic transcription. Furthermore, northern blot analysis indicates that PFR1 mRNA is distributed evenly in the early gastrula while PFR4 mRNA is predominantly localized to the presumptive ectoderm. At tail-bud stage, PFR1 transcripts are localized primarily to the neural and mesodermal tissues, PFR4 transcripts are most abundantly expressed in neural tissue, and more transcripts are detected in lateral plate mesoderm than in the somites. When animal cap explants of blastulae are cultured in the presence of mesoderm-inducing factors, PFR1 mRNA levels are maintained by bFGF and activin A. In contrast, PFR4 mRNA levels are significantly down-regulated. These observations suggest a differential expression and regulation of FGF receptors in early amphibian development.

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