Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene

1987 ◽  
Vol 7 (7) ◽  
pp. 2592-2596
Author(s):  
E K Hoffman ◽  
S P Trusko ◽  
N Freeman ◽  
D L George
Keyword(s):  
Promoter Region ◽  
Promoter Activity ◽  
Functional Characterization ◽  
Genetic Material ◽  
Bidirectional Promoter ◽  
Adrenocortical Tumor ◽  
Ras Gene ◽  
Untranslated Exon ◽  
Size Heterogeneity

We isolated and characterized the 5' region of the mouse c-Ki-ras gene, including a 5' untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5' ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5' region which exhibits bidirectional promoter activity.

scholarly journals Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.

1987 ◽  
Vol 7 (7) ◽  
pp. 2592-2596 ◽  
Author(s):  
E K Hoffman ◽  
S P Trusko ◽  
N Freeman ◽  
D L George
Keyword(s):  
Promoter Region ◽  
Promoter Activity ◽  
Functional Characterization ◽  
Genetic Material ◽  
Bidirectional Promoter ◽  
Adrenocortical Tumor ◽  
Ras Gene ◽  
Untranslated Exon ◽  
Size Heterogeneity

We isolated and characterized the 5' region of the mouse c-Ki-ras gene, including a 5' untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5' ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5' region which exhibits bidirectional promoter activity.

scholarly journals Functional characterization of Lilium lancifolium cold-responsive Zinc Finger Homeodomain (ZFHD) gene in abscisic acid and osmotic stress tolerance

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11508
Author(s):  
Yubing Yong ◽  
Yue Zhang ◽  
Yingmin Lyu
Keyword(s):  
Abscisic Acid ◽  
Transgenic Plants ◽  
Zinc Finger ◽  
Promoter Region ◽  
Salt Water ◽  
Functional Characterization ◽  
Regulatory Elements ◽  
Response Analysis ◽  
Lilium Lancifolium

Background. We have previously performed an analysis of the cold-responsive transcriptome in the mature leaves of tiger lily (Lilium lancifolium) by gene co-expression network identification. The results has revealed that a ZFHD gene, notated as encoding zinc finger homeodomain protein, may play an essential regulating role in tiger lily response to cold stress. Methods. A further investigation of the ZFHD gene (termed as LlZFHD4) responding to osmotic stresses, including cold, salt, water stresses, and abscisic acid (ABA) was performed in this study. Based on the transcriptome sequences, the coding region and 5′ promoter region of LlZFHD4 were cloned from mature tiger lily leaves. Stress response analysis was performed under continuous 4 °C, NaCl, PEG, and ABA treatments. Functional characterization of LlZFHD4 was conducted in transgenic Arabidopsis, tobacco, and yeast. Results. LlZFHD4 encodes a nuclear-localized protein consisting of 180 amino acids. The N-terminal region of LlZFHD4 has transcriptional activation activity in yeast. The 4 °C, NaCl, PEG, and ABA treatments induced the expression of LlZFHD4. Several stress- or hormone-responsive cis-acting regulatory elements (T-Box, BoxI. and ARF) and binding sites of transcription factors (MYC, DRE and W-box) were found in the core promoter region (789 bp) of LlZFHD4. Also, the GUS gene driven by LlZFHD4 promoter was up-regulated by cold, NaCl, water stresses, and ABA in Arabidopsis. Overexpression of LlZFHD4 improved cold and drought tolerance in transgenic Arabidopsis; higher survival rate and better osmotic adjustment capacity were observed in LlZFHD4 transgenic plants compared to wild type (WT) plants under 4 °C and PEG conditions. However, LlZFHD4 transgenic plants were less tolerant to salinity and more hypersensitive to ABA compared to WT plants. The transcript levels of stress- and ABA-responsive genes were much more up-regulated in LlZFHD4 transgenic Arabidopsis than WT. These results indicate LlZFHD4 is involved in ABA signaling pathway and plays a crucial role in regulating the response of tiger lily to cold, salt and water stresses.

scholarly journals Functional Characterization of Variants inMC4RGene Promoter Region Found in Obese Children

2014 ◽  
Vol 99 (5) ◽  
pp. E931-E935 ◽  
Author(s):  
Karen Mei Ling Tan ◽  
Shu Qin Delicia Ooi ◽  
Siong Gim Ong ◽  
Charmaine Shuyi Kwan ◽  
Raymond Ming En Chan ◽  
...  
Keyword(s):  
Promoter Region ◽  
Functional Characterization ◽  
Obese Children

scholarly journals Functional characterization of an inducible bidirectional promoter from Fusarium oxysporum f. sp. cubense

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ashutosh Dash ◽  
Vartika Gurdaswani ◽  
Jacinta S. D’Souza ◽  
Siddhesh B. Ghag
Keyword(s):  
Fusarium Oxysporum ◽  
Functional Characterization ◽  
Bidirectional Promoter

scholarly journals Functional Characterization of 4 Polymorphisms in Promoter Region of Hepatic Lipase Gene

2000 ◽  
Vol 20 (5) ◽  
pp. 1335-1339 ◽  
Author(s):  
Ferdinand M. van ′t Hooft ◽  
Björn Lundahl ◽  
Francesca Ragogna ◽  
Fredrik Karpe ◽  
Gunilla Olivecrona ◽  
...  
Keyword(s):  
Promoter Region ◽  
Hepatic Lipase ◽  
Functional Characterization ◽  
Lipase Gene ◽  
Hepatic Lipase Gene

scholarly journals Identification and functional characterization of the promoter region of the human organic anion transporting polypeptide gene

Hepatology ◽  
1997 ◽  
Vol 26 (4) ◽  
pp. 991-997 ◽  
Author(s):  
G Kullak-Ublick ◽  
U Beuers ◽  
C Fahney ◽  
B Hagenbuch ◽  
P J Meier ◽  
...  
Keyword(s):  
Promoter Region ◽  
Organic Anion ◽  
Functional Characterization ◽  
Organic Anion Transporting Polypeptide

Functional characterization of genetic polymorphisms identified in the human cytochrome P450 4F12 (CYP4F12) promoter region

2004 ◽  
Vol 67 (12) ◽  
pp. 2231-2238 ◽  
Author(s):  
Christelle Cauffiez ◽  
Florian Klinzig ◽  
Emmanuel Rat ◽  
Gilles Tournel ◽  
Delphine Allorge ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Genetic Polymorphisms ◽  
Promoter Region ◽  
Functional Characterization ◽  
Human Cytochrome

scholarly journals Functional characterization of the promoter region of bovine Neuropeptide Y (NPY) gene

2010 ◽  
Vol 1 (1) ◽  
pp. 213-213
Author(s):  
T Alam ◽  
B Bahar ◽  
S Waters ◽  
M McGee ◽  
T Sweeney
Keyword(s):  
Neuropeptide Y ◽  
Promoter Region ◽  
Functional Characterization

Functional characterization of the promoter region of human TNFAIP1 gene

2009 ◽  
Vol 37 (4) ◽  
pp. 1699-1705 ◽  
Author(s):  
Mingjun Liu ◽  
Zhenhua Sun ◽  
Aidong Zhou ◽  
Hong Li ◽  
Liping Yang ◽  
...  
Keyword(s):  
Promoter Region ◽  
Functional Characterization

scholarly journals Functional characterization of the human phosphodiesterase 7A1 promoter

2003 ◽  
Vol 373 (3) ◽  
pp. 835-843 ◽  
Author(s):  
Mònica TORRAS-LLORT ◽  
Fernando AZORÍN
Keyword(s):  
T Cells ◽  
Promoter Region ◽  
Promoter Activity ◽  
Cpg Island ◽  
Functional Characterization ◽  
Regulatory Elements ◽  
Dominant Negative ◽  
Nuclear Factor ◽  
Jurkat T Cells

In this paper, the human phosphodiesterase 7A1 (hPDE7A1) promoter region was identified and functionally characterized. Transient transfection experiments indicated that a 2.9 kb fragment of the hPDE7A1 5′-flanking region, to position −2907, has strong promoter activity in Jurkat T-cells. Deletion analysis showed that the proximal region, up to position −988, contains major cis-regulatory elements of the hPDE7A1 promoter. This minimal promoter region contains a regulatory CpG island which is essential for promoter activity. The CpG island contains three potential cAMP-response-element-binding protein (CREB)-binding sites that, as judged by in vivo dimethyl sulphate (DMS) footprinting, are occupied in Jurkat T-cells. Moreover, over-expression of CREB results in increased promoter activity, but, on the other hand, promoter activity decreases when a dominant-negative form of CREB (KCREB) is over-expressed. In vivo DMS footprinting strongly indicates that other transcription factors, such Ets-2, nuclear factor of activated T-cells 1 (NFAT-1) and nuclear factor κB (NF-κB), might also contribute to the regulation of hPDE7A1 promoter. Finally, hPDE7A1 promoter was found to be induced by treatment with PMA, but not by treatment with dibutyryl cAMP or forskolin. These results provide insights into the factors and mechanisms that regulate expression of the hPDE7A gene.

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