A plant DNA-binding protein that recognizes 5-methylcytosine residues

1989 ◽  
Vol 9 (3) ◽  
pp. 1351-1356
Author(s):  
D L Zhang ◽  
K C Ehrlich ◽  
P C Supakar ◽  
M Ehrlich
Keyword(s):  
Dna Binding ◽  
Dna Sequence ◽  
Base Pair ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Sequence Specificity ◽  
Dna Sequence Specificity ◽  
Plant Dna ◽  
Related Family ◽  
Nuclear Extracts

A novel, 5-methylcytosine-specific, DNA-binding protein, DBP-m, has been identified in nuclear extracts of peas. DBP-m specifically recognizes 5-methylcytosine residues in DNA without appreciable DNA sequence specificity, unlike a mammalian DNA-binding protein (MDBP), which recognizes 5-methylcytosine residues but only in a related family of 14-base-pair sequences.

scholarly journals A plant DNA-binding protein that recognizes 5-methylcytosine residues.

1989 ◽  
Vol 9 (3) ◽  
pp. 1351-1356 ◽  
Author(s):  
D L Zhang ◽  
K C Ehrlich ◽  
P C Supakar ◽  
M Ehrlich
Keyword(s):  
Dna Binding ◽  
Dna Sequence ◽  
Base Pair ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Sequence Specificity ◽  
Dna Sequence Specificity ◽  
Plant Dna ◽  
Related Family ◽  
Nuclear Extracts

A novel, 5-methylcytosine-specific, DNA-binding protein, DBP-m, has been identified in nuclear extracts of peas. DBP-m specifically recognizes 5-methylcytosine residues in DNA without appreciable DNA sequence specificity, unlike a mammalian DNA-binding protein (MDBP), which recognizes 5-methylcytosine residues but only in a related family of 14-base-pair sequences.

scholarly journals Adenoviral E2 IVa2 protein interacts with L4 33K protein and E2 DNA-binding protein

2013 ◽  
Vol 94 (6) ◽  
pp. 1325-1334 ◽  
Author(s):  
Yadvinder S. Ahi ◽  
Sai V. Vemula ◽  
Suresh K. Mittal
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Indirect Evidence ◽  
Dna Binding Protein ◽  
Immunogold Electron Microscopy ◽  
Genome Packaging ◽  
Nuclear Extracts ◽  
Infected Cells ◽  
Unique Vertex

Adenovirus (AdV) is thought to follow a sequential assembly pathway similar to that observed in dsDNA bacteriophages and herpesviruses. First, empty capsids are assembled, and then the genome is packaged through a ring-like structure, referred to as a portal, located at a unique vertex. In human AdV serotype 5 (HAdV5), the IVa2 protein initiates specific recognition of viral genome by associating with the viral packaging domain located between nucleotides 220 and 400 of the genome. IVa2 is located at a unique vertex on mature capsids and plays an essential role during genome packaging, most likely by acting as a DNA packaging ATPase. In this study, we demonstrated interactions among IVa2, 33K and DNA-binding protein (DBP) in virus-infected cells by in vivo cross-linking of HAdV5-infected cells followed by Western blot, and co-immunoprecipitation of IVa2, 33K and DBP from nuclear extracts of HAdV5-infected cells. Confocal microscopy demonstrated co-localization of IVa2, 33K and DBP in virus-infected cells and also in cells transfected with IVa2, 33K and DBP genes. Immunogold electron microscopy of purified HAdV5 showed the presence of IVa2, 33K or DBP at a single site on the virus particles. Our results provide indirect evidence that IVa2, 33K and DBP may form a complex at a unique vertex on viral capsids and cooperate in genome packaging.

scholarly journals Identification of a 30-Base Pair Regulatory Element and Novel DNA Binding Protein That Regulates the Human GLUT4 Promoter in Transgenic Mice

2000 ◽  
Vol 275 (31) ◽  
pp. 23666-23673 ◽  
Author(s):  
Katherine M. Oshel ◽  
John B. Knight ◽  
Kim T. Cao ◽  
Martin V. Thai ◽  
Ann Louise Olson
Keyword(s):  
Dna Binding ◽  
Transgenic Mice ◽  
Base Pair ◽  
Binding Protein ◽  
Regulatory Element ◽  
Dna Binding Protein

A Novel DNA Binding Protein-Based Platform for Electrochemical Detection of miRNA

The Analyst ◽  
2021 ◽  
Author(s):  
Muhammad Umer ◽  
Nahian Binte Aziz ◽  
Rabbee G. Mahmudunnabi ◽  
Yoon-Bo Shim ◽  
Carlos Salomon ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Sequence ◽  
Electrochemical Detection ◽  
Dna Polymerase ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Sandwich Type

We present a novel amplification-free sandwich type platform assay for electrochemical detection of miRNA. The assay is based on T4 DNA polymerase mediated synthesis of p53 binding DNA sequence at...

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